User Panel

My Panel

Bookmark Science Articles

colony Protocols

Search results for: colony

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Bacterial Colony PCR - http://www.cbs.umn.edu/~kclark/protocols/bactPCR.html

Category: PCR Protocols: Colony PCR

Bacterial Colony PCR. Karl Clark, University of Minnesota. - [Read Bacterial Colony PCR]

Colony Hybridization Protocol - http://cmgm.stanford.edu/~vesicle/protocols/hybrid.html

Category: RNA Protocols: cDNA Libraries Library

Methods and procedure for Colony Hybridization. Vesicle Trafficking Home. - [Read Colony Hybridization Protocol]

Colony Hybridization Protocol - http://www.fhcrc.org/science/labs/fero/Protocols/ColonyHybridization.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols

Protocol for colony hybridization. - [Read Colony Hybridization Protocol]

Colony PCR - http://www.biology.lsa.umich.edu/research/labs/maddock/protocols/PCR/colony_pcr.html

Category: PCR Protocols: Colony PCR

Identification of Bacterial Colonies using PCR. Colony PCR Method and Technique. Maddock Lab, The University of Michigan. - [Read Colony PCR]

Colony PCR Protocol - http://www.enterococcus.ouhsc.edu/ColonyPCRProtocol.asp

Category: PCR Protocols: Colony PCR

PCR Reaction Conditions for Colony PCR. Contributed by Lynn Hancock. Enterococcus Research Site, The Board of Regents of the University of Oklahoma. - [Read Colony PCR Protocol]

Colony PCR Protocol II - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4141

Category: PCR Protocols: Colony PCR

Protocol describes the use of PCR to screen for bacteria that carry recombinant plasmids. The PCR can be carried out using the same primers as for amplification of the cloned insert. To determine the orientation of the insert, a third, insert-specific primer that is asymmetrically distanced from the clonal insertion site can be used. - [Read Colony PCR Protocol II]

Determining Plating Efficiency in Yeast: Direct Method - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4185

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Direct method for determining efficiency in yeast. The plating efficiency of a strain is a measure of the percentage of cells in a culture that are capable of forming colonies (colony forming . - [Read Determining Plating Efficiency in Yeast: Direct Method]

ES Cell Culture Protocols - ESGRO - http://www.esgro-lif.com/esgro/protocols.htm

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: ES Cell Growth Media and Requirements

ES Cell Culture Protocols - EXCELLENT GUIDE. Harvesting and DNA Preparation, Colony Picking, Electroporation, ES Cell Culture using ESGROÂ® and Feeder Cell Layer. ESGRO. - [Read ES Cell Culture Protocols - ESGRO]

Hematopoietic Colony Forming Cell Assays Protocol - http://www.fhcrc.org/science/labs/fero/Protocols/HematopoieticCFC.pdf

Category: Stem Cell Protocols: Stem Cell Culture Protocols

Protocol for the hematopoietic colony forming cell assay. - [Read Hematopoietic Colony Forming Cell Assays Protocol]

Hepatoma Cell Cultures as In Vitro Models for Hepatotoxicity - http://embryo.ib.amwaw.edu.pl/invittox/prot/13.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Liver Toxicity Assays Protocols

This test is designed to detect irreversible toxic effects on both cell growth and survival, by the evaluation of colony-forming (CF) efficiency, in hepatoma cell lines derived from man, rat and mouse. - [Read Hepatoma Cell Cultures as In Vitro Models for Hepatotoxicity]

How to Avoid Pitfalls and Keep Out of Trouble in the Neurospora Lab - http://www.fgsc.net/neurosporaprotocols/How%20to%20avoid%20pitfalls%20and%20keep%20out%20of%20trouble.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to avoid pitfalls and keep out of trouble in the Neurospora lab. Includes: Culture, incubation, storage, and disposal; Stock preservation; Spore and colony counts; Making crosses and recovering progeny; Use of heterokaryons for allelism tests; Use of unordered asci to estimate the relation of translocation breakpoints to centromeres. - [Read How to Avoid Pitfalls and Keep Out of Trouble in the Neurospora Lab]

Natural Pathogens of Laboratory Mice and Rats - http://members.cox.net/microinjectionworkshop/technical/pathogen.html

Category: Mouse Protocols

For all the transgenic or other mouse researchers out there who have had to explain why colony infections are not just bad for the welfare of the animals concerned, but may invalidate the ongoing studies as well. - [Read Natural Pathogens of Laboratory Mice and Rats]

Planning an Effective Mouse Colony - http://members.cox.net/microinjectionworkshop/technical/tgcolny1.html

Category: Mouse Protocols: Mouse Embryology Protocols

Considerations in planning an effective mouse colony for transgenic production. Includes: Mouse strain; Colony size; Efficiency of superovulation; Parental Suitability; Pseudo-pregnancy. - [Read Planning an Effective Mouse Colony]

Sampling of Aspergillus spp Spores in Air Protocol - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./airprotocol.html~main

Category: Fungi Protocols: Aspergillus Protocols

Protocol allows the isolation and enumerate Aspergillus spp spores in air. Includes: Sampling Procedure; Sampling Location; Selection of Sampling Time; Sampling Steps; Laboratory Procedure; Enumerating the Colony Forming Units. - [Read Sampling of Aspergillus spp Spores in Air Protocol]

Single tube confirmation PCR protocol - http://www-sequence.stanford.edu/group/yeast_deletion_project/single_tube_protocol.html

Category: PCR Protocols: Colony PCR

The following colony PCR protocol has been designed to be performed in individual reaction tubes. We usually test three colonies from each transformation along with a wild-type control. A series of five different PCR tests are performed on each colony to - [Read Single tube confirmation PCR protocol]

Streaking Yeast Stocks Protocols - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast1.html

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Some yeast strains are unstable (e. g., small YAC-bearing strains) and need to be repurified by streaking on an agar plate and then verifying the genetic content of the isolated colony before proceeding. In cases where the strain is unstable, plan to streak the cells onto the selective medium to retain the desired stock, (however, most strains can be streaked onto the complete medium, YPD). - [Read Streaking Yeast Stocks Protocols]

Yeast and Bacterial Colony PCR Protocol. - http://www.upstate.edu/biochem/amberg/protocols/colony_pcr.html

Category: PCR Protocols: Colony PCR

Yeast and Bacterial Colony PCR Protocol. David Amberg SUNY Upstate Medical University - [Read Yeast and Bacterial Colony PCR Protocol.]

Yeast Colony PCR - http://www.fhcrc.org/science/labs/breeden/Methods/colonyPCR.html

Category: PCR Protocols: Colony PCR

Yeast colony PCR (analytic). Breeden Lab Fred Hutchinson Cancer Research Center - [Read Yeast Colony PCR]

Yeast Colony PCR - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4170

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast PCR Protocols

This method for yeast colony PCR is used to test for the presence of target sequences in cells. - [Read Yeast Colony PCR]

Yeast Colony PCR Protocol - http://www.fhcrc.org/science/labs/gottschling/yeast/ypcr.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast PCR Protocols

Protocol for Yeast colony PCR. Includes: Quick SDS extraction protocol. - [Read Yeast Colony PCR Protocol]

Yeast Colony PCR Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4170

Category: PCR Protocols: Colony PCR

Protocol for yeast colony PCR. This method for yeast colony PCR is used to test for the presence of target sequences in cells. - [Read Yeast Colony PCR Protocol]

Articles (1-4 of 4)

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

[1-4]