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A Concise Guide to cArray Hybridization Procedures - DNA Microarray Analysis - http://www.tigr.org/tdb/microarray/conciseguide.html

Category: DNA Microarray Protocols

Hegde et al., Array Fabrication, microarray Printing, Probe Preparation and Hybridization, RNA Extraction, RNA labeling, Data Collection, Normalization, and Analysis.Institute for Genomic Research, Rockville, MD - [Read A Concise Guide to cArray Hybridization Procedures - DNA Microarray Analysis]

A Concise Guide to cDNA Microarray Analysis â€“ II PDF. - http://pga.tigr.org/PDF/BiotechniquesCookbook_II.pdf

Category: DNA Microarray Protocols

Micro Array Fabrication, Probe Preparation and Hybridization, and Data Collection, Normalization and Analysis. Hegde, et al. biotechniques 2000. - [Read A Concise Guide to cDNA Microarray Analysis â€“ II PDF.]

Analysis of Flow Cytometry Data Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E661C2CE22FCCB1C294CD8376FD8830&objectid=6674E762AC837B13929440A1F32AAEF0

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Flow Cytometry Data Analysis Protocols

Provides several approaches to flow cytometry data analysis. Frequency determinations based on analysis of single-parameter fluorescence histograms and dual-parameter contour plots are presented. Steps are described for calculating values for signal-to-noise ratios when logarithmic amplification is used for data collection. - [Read Analysis of Flow Cytometry Data Protocol]

Antigen Design and Sera Purification - http://www.sigmaaldrich.com/Brands/Sigma_Genosys/Custom_Antisera/Key_Resources/Antigen_Design.html

Category: Immunology: Antigen

Antigen Design and Sera Purification. Custom antisera. Sigma Aldrich. Peptide Selection and Design, Coupling Strategy # Selecting the Protein Carrier, Multiple Antigenic Peptides (MAPs), Choice of Host, Adjuvant, Immunization, & Sera Collection, Antisera Purification, Ammonium Sulfate Precipitation, Protein A/G, Immunoaffinity Purification. - [Read Antigen Design and Sera Purification]

Biomethodology of the Guinea Pig - http://research.uiowa.edu/animal/?get=g_pig

Category: Animal Research Techniques

Guinea Pig Biology, Sources Ordering, Behavior, Basic Husbandry, Identification, Handling, Breeding, Restraint Devices, Sexing, Transport, Anesthesia, Euthanasia, Rodent Surgery, Blood Collection, Administration of Fluid. Univ. Iowa. Michael Parker. - [Read Biomethodology of the Guinea Pig]

Biomethodology of the Mouse - http://research.uiowa.edu/animal/?get=mouse

Category: Animal Research Techniques

Mus mouse Biology, Sources Ordering, Behavior, Basic Husbandry, Identification, Handling, Breeding, Restraint Devices, Sexing, Transport, Anesthesia, Euthanasia, Rodent Surgery, Blood Collection, Administration of Fluid. Univ. Iowa. Michael Parker. - [Read Biomethodology of the Mouse]

Biomethodology of the Rabbit - http://research.uiowa.edu/animal/?get=rabbit

Category: Animal Research Techniques

Rabbit Biology, Sources Ordering, Behavior, Basic Husbandry, Identification, Handling, Breeding, Restraint Devices, Sexing, Transport, Anesthesia, Euthanasia, Rodent Surgery, Blood Collection, Administration of Fluid. Univ. Iowa. Michael Parker. - [Read Biomethodology of the Rabbit]

Biomethodology of the Rat - http://research.uiowa.edu/animal/?get=rat

Category: Animal Research Techniques

Rat Biology, Sources Ordering, Behavior, Basic Husbandry, Identification, Handling, Breeding, Restraint Devices, Sexing, Transport, Anesthesia, Euthanasia, Rodent Surgery, Blood Collection, Administration of Fluid. Univ. Iowa. Michael Parker. - [Read Biomethodology of the Rat]

Blood Collection by Tail Bleeding Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4388

Category: Mouse Protocols: Mouse Surgical Procedures Protocols

Protocol describes a method for collection of blood from the tail of a mouse. The blood can then be used for analysis. - [Read Blood Collection by Tail Bleeding Protocol]

Chromatin immunoprecipitation protocol to analyze histone modifications in Arabidopsis thaliana - http://vkp.leeds.ac.uk/Drive/Drive/ViewFile?include=y&component=security&action=Start&nextAction=gotoDoc&doc=494824#search=%22chromatin%20immunoprecipitation%20protocol%22

Category: Epigenetics and Methylation Protocols: ChIP Chromatin Immunoprecipitation Protocols

Chromatin immunoprecipitation protocol to analyze histone modifications in Arabidopsis thaliana. Werner Aufsatz, Matzke Lab. Gregor Mendel Institute of Molecular Plant Biology, Austria. Includes Chromatin Crosslinking, Chromatin preparation, Pre-clearing and immuno precipitation (IP), Collection,washes and elution of immune complexes, Reverse crosslinking and DNA cleanup - [Read Chromatin immunoprecipitation protocol to analyze histone modifications in Arabidopsis thaliana]

Collection and Storage of Cotton Leaf Tissue - http://wheat.pw.usda.gov/~lazo/methods/lazo/collect.html

Category: Plant Biology Protocols

Protocol for the collection and storage of cotton leaf tissue. - [Read Collection and Storage of Cotton Leaf Tissue]

Collection of Early Mouse Embryos for RNAi Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4510

Category: RNAi Technology Protocols: RNAi Mouse Embryos and Oocytes Protocols

Protocol describes a method to collect early embryos from 6-week-old mice. Subsequently, the isolated embryos can be injected with double-stranded RNA to induce knockdown of a gene of interest. - [Read Collection of Early Mouse Embryos for RNAi Protocol]

Collection of Mouse Oocytes for RNAi Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4509

Category: RNAi Technology Protocols: RNAi Mouse Embryos and Oocytes Protocols

Protocol describes a method to collect oocytes from 6-week-old mice. Subsequently, the isolated oocytes can be injected with double-stranded RNA to induce knockdown of a gene of interest. - [Read Collection of Mouse Oocytes for RNAi Protocol]

Collection of Mouse Peritoneal Cells Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/mousepathology/CollectPerC.html

Category: Mouse Protocols

Protocol for the collection of mouse peritoneal cells. - [Read Collection of Mouse Peritoneal Cells Protocol]

Collection of Protein Purification Links - http://www.ebioinfogen.com/PROTPURI.htm

Category: Protein Protocols: Protein Purification and Identification

Collection of Protein Purification Links - [Read Collection of Protein Purification Links]

Collection of Specimens for Detection of Measles Virus RNA by PCR Protocol - http://www.vidrl.org.au/labsandunits/measles/forms/Specimen%20protocol%20OCT%2003.pdf

Category: PCR Protocols

Protocol for collection of specimens for detection of measles virus RNA by PCR. - [Read Collection of Specimens for Detection of Measles Virus RNA by PCR Protocol]

Drosophila Hard Agar Medium for Egg Collection Plates Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/hardagar.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila hard agar medium for egg collection plates. - [Read Drosophila Hard Agar Medium for Egg Collection Plates Protocol]

Ethics, Guidelines for Animal Research PDF - http://www.brl.uic.edu/courses/TNotes05.pdf

Category: Animal Research Techniques

Needs of animals, Ventilation, lighting and temperature considerations. Cage size/density. Handling. Blood Collection and guidlines. EXCELLENT GUIDE GC470 ESSENTIALS FOR ANIMAL RESEARCH BRL Univ. Illinois. - [Read Ethics, Guidelines for Animal Research PDF]

Extraction and Solubilization of Total Protein from Microorganisms Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4224

Category: Bacterial Cell Culture Protocols

The growth conditions of microbial cell cultures and the time of sample collection should be optimized and standardized when growing cells for protein extraction. Because cells may excrete proteases and other extracellular enzymes, and compounds in the medium may interfere with extraction, wash cultures with an isotonic buffer, such as PBS or sucrose before solubilization. - [Read Extraction and Solubilization of Total Protein from Microorganisms Protocol]

FISH Protocols for Drosophila - http://www.utoronto.ca/krause/FISH.html

Category: Drosophila Protocols: Drosophila Genetics Protocols

FISH protocols for Drosophila. Includes: RNA Probe Preparation; Embryo Collection and Fixation; Single FISH on Drosophila embryos; Post-Fixation, Hybridization and Post-Hybridization Washes; Development of FISH Signal; Storage, Mounting and Viewing of Samples; Double FISH on Drosophila Embryos; RNA-Protein Double Labeling; FISH on Dissected Tissues. - [Read FISH Protocols for Drosophila]

Flow cytometric determination of leukocyte surface antigens in whole blood - http://pingu.salk.edu/flow/protocols/wholeblood.html

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Flow cytometric determination of leukocyte surface antigens in whole blood. Quantitation of cell surface antigens in whole blood with the flow cytometer is very simple and requires:
1. Blood collection; 2. Addition of antibody; 3. Calibration of the flow cytometer; 4. Making measurements. - [Read Flow cytometric determination of leukocyte surface antigens in whole blood]

Flow Cytometric Determination of Leukocyte Surface Antigens in Whole Blood Protocol - http://pingu.salk.edu/flow/protocols/wholeblood.html

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Flow Cytometry Immunofluorescence Staining Protocols

Protocol for flow cytometric determination of leukocyte surface antigens in whole blood. Includes: Blood collection; Add antibody to the blood; Make measurements. - [Read Flow Cytometric Determination of Leukocyte Surface Antigens in Whole Blood Protocol]

Live Imaging of Caenorhabditis elegans: Observation of Nematodes and Data Collection - http://www.cshprotocols.org/cgi/content/extract/2006/29/pdb.ip18

Category: Microscopy Protocols: In Vivo Imaging Protocols

Live nematodes can be studied at all levels of microscopic resolution. Data collection includes information on: Photographic Records, Video Microscopy,Multiple-Focal-Plane Time-Lapse Recording Systems and Digital Imaging. - [Read Live Imaging of Caenorhabditis elegans: Observation of Nematodes and Data Collection]

Live Imaging of Caenorhabditis elegans: Observation of Nematodes and Data Collection Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/29/pdb.ip18

Category: C. elegans Protocols

At low magnification, dissection microscopes are useful, whereas at higher magnification and resolution, a wide range of optical methods can be used, including differential interference contrast (DIC) optics, phase contrast, fluorescence, polarized light, confocal, and dark field. - [Read Live Imaging of Caenorhabditis elegans: Observation of Nematodes and Data Collection Protocol]

Long Term Lambda Phage Storage Protocol - http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda6.html

Category: Cell Culture Protocols: Cell Storing and Cell Freezing Protocols

No special treatment is required to prepare a lysate for the active collection. The following procedure should be used for long-term storage of lambda clones in the archival collections. The phage are diluted in media containing 7% DMSO and frozen at -80 degrees C. - [Read Long Term Lambda Phage Storage Protocol]

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