cold Protocols

Category: Bioinformatics

Using AFPredictor, it was demonstrated that ‘ordered surface carbons’ (OSCs) are a distinguishing feature of AFPs and, more specifically, their ice-binding surfaces. AFPredictor identified AFPs from within a large set of structures with greater than 99% specificity. Furthermore, it was used to identify a novel ice-binding protein by screening a library of homology modeled structures based on cDNA sequences obtained from cold-acclimated winter rye (Secale cereale). - [Read A Computational Screening protocol for Antifreeze/Ice-Structuring Proteins]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

Detection of Alu by PCR - A Human DNA Fingerprinting Lab Protocol - 1994 Cold Spring Harbor Laboratory DNA Learning Center - [Read Detection of Alu by PCR]

Category: PCR Protocols

Detection of Alu by PCR - A Human DNA Fingerprinting Lab Protocol - 1994 Cold Spring Harbor Laboratory DNA Learning Center - [Read Detection of Alu by PCR]

Category: Protein Protocols: Protein Precipitation Procols

Fractional Precipitation of Proteins by Ammonium Sulfate Simpson Cold Spring Harbor Protocols.2006; 2006: pdb.prot4309 - [Read Fractional Precipitation of Proteins by Ammonium Sulfate - Subscription]

Category: Bacteria Transformation Protocols

Protocol reproducibly generates competent cultures of E. coli that yield 1 x 108 to 3 x 108 transformed colonies/µg of plasmid DNA. The protocol works optimally when the bacterial culture is grown at 18°C. If a suitable incubator is not available, a standard bacterial shaker can be set up in a 4°C cold room and regulated to 18°C. - [Read Preparation and Transformation of Competent E. Coli: "Ultra-Competent" Cells Protocol]

Category: E Coli Protocols: E coli Transformation Protocols

Protocol reproducibly generates competent cultures of E. coli that yield 1 x 108 to 3 x 108 transformed colonies/µg of plasmid DNA. The protocol works optimally when the bacterial culture is grown at 18°C. If a suitable incubator is not available, a standard bacterial shaker can be set up in a 4°C cold room and regulated to 18°C. - [Read Preparation and Transformation of Competent E. Coli: "Ultra-Competent" Cells Protocol]

Category: Cell Culture Protocols

Protocol on [3H]Thymidine labeling. Protocol includes: Explant Culture Medium; 100X (cold) Thymidine; Dektol Developer; Fixer - [Read [3H]Thymidine Labeling Protocol]