cohesive Protocols

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Attachment of Linkers or Adaptors for Construction of cDNA Libraries - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3310

Category: DNA Protocols: cDNA Library Protocols

This stage achieves four goals: polishing the ends of double-stranded DNA, ligation of synthetic linkers or adaptors, digestion of the attached linkers to create cohesive termini, and preparing the cDNA for cloning. - [Read Attachment of Linkers or Adaptors for Construction of cDNA Libraries]

Cohesive End Ligation - http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/protocols/ligation.html

Category: Molecular Biology Protocols: DNA Ligation Protocols

DNA ligation protocol with excellent notes and tips. Chen Lab, Univ. College London. - [Read Cohesive End Ligation]

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DNA Ligation Protocol

The DNA Ligation protocol described here contains the steps required to join together using ligase enzyme both plasmid DNA and insert DNA fragments in order to create a new plasmid. This new ligated plasmid can be transformed after into competent bacteria to produce DNA for mini, midi or maxi-prep isolation.