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Chromatin immunoprecipitation protocol to analyze histone modifications in Arabidopsis thaliana - http://vkp.leeds.ac.uk/Drive/Drive/ViewFile?include=y&component=security&action=Start&nextAction=gotoDoc&doc=494824#search=%22chromatin%20immunoprecipitation%20protocol%22

Category: Epigenetics and Methylation Protocols: ChIP Chromatin Immunoprecipitation Protocols

Chromatin immunoprecipitation protocol to analyze histone modifications in Arabidopsis thaliana. Werner Aufsatz, Matzke Lab. Gregor Mendel Institute of Molecular Plant Biology, Austria. Includes Chromatin Crosslinking, Chromatin preparation, Pre-clearing and immuno precipitation (IP), Collection,washes and elution of immune complexes, Reverse crosslinking and DNA cleanup - [Read Chromatin immunoprecipitation protocol to analyze histone modifications in Arabidopsis thaliana]

Dye Terminator Cleanup for Automated DNA Sequencing Reactions Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4101

Category: DNA Protocols: DNA Sequencing Protocols

Protocol describes a simple procedure for purifying sequencing reactions using the MagneSil particles from Promega. - [Read Dye Terminator Cleanup for Automated DNA Sequencing Reactions Protocol]

Fan Lab Bisulfite Treatment of Genomic DNA - http://www.genetics.ucla.edu/labs/fan/Protocols_Bisulfite.htm

Category: Epigenetics and Methylation Protocols: Bisulfite Treatment Methylation Assay Protocol

A detailed protocol for bisulfite treatment of DNA. DNA is first digested with a restriction enzyme. DNA is denauted at 97C for 5min. Bisulfite protocol is included. Also DNA purification using the Promega Wizard DNA Cleanup kit in indicated. Fan Lab. UCL - [Read Fan Lab Bisulfite Treatment of Genomic DNA]

Microarray Protocol for Agilent Inkjet-Deposited Presynthesized Oligo Arrays - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000179.pdf?pid=PP00000179

Category: Microarray Protocols: Microarray Hybridization Protocols

Protocol details the preparation of fluorescently labeled target samples and hybridization of these samples to a microarray of Agilent inkjet-deposited presynthesized oligonucleotides. The procedure requires a minimum of 3 µg of purified total RNA as starting material. Includes: cDNA Synthesis; Fluorescent cRNA Synthesis; cRNA Precipitation and Cleanup; cRNA Quantification; Hybridization; Washing. - [Read Microarray Protocol for Agilent Inkjet-Deposited Presynthesized Oligo Arrays]

PCR cleanup using shrimp alkaline phosphotase (SAP) - http://golab.unl.edu/protocols/SAP.html

Category: DNA Protocols: SAP Protocols

PCR cleanup using shrimp alkaline phosphotase (SAP). Orti Lab. - [Read PCR cleanup using shrimp alkaline phosphotase (SAP)]

PCR Cleanup with AMPure magnetic beads - http://plantpath.wisc.edu/~amyc/Protocols/protocols%20-%20PCR.htm

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

PCR Cleanup with AMPure magnetic beads. Charkowski Lab. - [Read PCR Cleanup with AMPure magnetic beads]

PCR Cleanup with AMPure magnetic beads - http://plantpath.wisc.edu/~amyc/Protocols/protocols%20-%20PCR.htm

Category: PCR Protocols

PCR Cleanup with AMPure magnetic beads. Charkowski Lab. - [Read PCR Cleanup with AMPure magnetic beads]

Protein Precipitation Microplate - http://www.htslabs.com/HTML/Wellplates/Applications/protein_precipitation_microplate.htm

Category: Protein Protocols: Protein Precipitation Procols

Laboratory sample cleanup is a necessary part for analytical preparation analysis. The removal of Contaminants such as proteins, cell debris and other materials is an important step. Typically this has been done by using Acetonitrile and then Centrifugation to pellet the debris leaving the clean supernant. After this process supernatant can be used for further analysis by HPLC, GC, MS and other analysis tandem methods. HTS Labs. - [Read Protein Precipitation Microplate]

Transcript Profiling by Microarray Analysis Protocol. - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000019.pdf?pid=PP00000019

Category: Microarray Protocols: Microarray Hybridization Protocols

Protocol details the preparation of fluorescently labeled target samples and hybridization of these samples to a microarray of Agilent inkjet-deposited cDNAs. The procedure requires a minimum of 5 mg of purified total RNA as starting material. Includes: First Strand cDNA Synthesis; Second Strand cDNA Synthesis; cDNA Cleanup and Precipitation; In vitro Transcription; Cleanup and Quantification of in vitro Transcribed RNA; Fluorescent Labeling of the Target Samples. - [Read Transcript Profiling by Microarray Analysis Protocol.]