chloroplasts Protocols

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Determination of Chlorophyll Content in Chloroplasts - http://www.bio.com/protocolstools/protocol.jhtml?id=p1285

Category: Plant Biology Protocols: Photosynthesis and Respiration Protocols

This protocol describes how to determine the chlorophyll content of chloroplasts using a spectrophotometric assay. - [Read Determination of Chlorophyll Content in Chloroplasts]

Import Assays Into Chloroplasts: Integration or Transport into Thylakoids - http://www.hos.ufl.edu/clineweb/Protocols/ProteinImport.htm

Category: Protein Protocols: Protein Trafficking

Import assays into chloroplasts. Includes: Preparation of chloroplasts and lysates; Transport of lumenal proteins. - [Read Import Assays Into Chloroplasts: Integration or Transport into Thylakoids]

Isolation of Chloroplasts from Spinach Leaves Protocol - http://homepages.gac.edu/~cellab/chpts/chpt8/ex8-1.html

Category: Plant Biology Protocols: Photosynthesis and Respiration Protocols

Protocol describing the isolation of chloroplasts from spinach leaves. - [Read Isolation of Chloroplasts from Spinach Leaves Protocol]

Photosynthesis and Respiration - Introduction - http://homepages.gac.edu/~cellab/chpts/chpt8/intro8.html

Category: Plant Biology Protocols: Photosynthesis and Respiration Protocols

The physiological reactions of mitochondria and chloroplasts can be reduced to a series of electron transfers, catalyzed by specific enzymes found within the organelles. Thus, we can study the component processes of photosynthesis and respiration by isolating the organelles and measuring specific enzyme activity associated with that organelle. - [Read Photosynthesis and Respiration - Introduction]

Photosynthesis by Respirometry - http://homepages.gac.edu/~cellab/chpts/chpt8/ex8-8.html

Category: Plant Biology Protocols: Photosynthesis and Respiration Protocols

Protocol describing photosynthesis by respirometry. Includes characteristics of isolated chloroplasts. - [Read Photosynthesis by Respirometry]

Preparation of Arabidopsis Chloroplasts Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4681

Category: Plant Biology Protocols: Arabidopsis Tissue Culture

It is desirable to prepare subcellular fractions, either to localize proteins or to improve the sensitivity of protein detection. This procedure describes the enrichment of chloroplasts from Arabidopsis. - [Read Preparation of Arabidopsis Chloroplasts Protocol]