chlorophyll Protocols

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Characterization of Cells by Flow Cytometry Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000018.pdf?pid=PP00000018

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol uses specific antibodies coupled to one of four fluorochromes: fluorescein isothiocyanate (FITC), R-phycoerythrin (PE), peridinin chlorophyll-a (PcP), and allophycocyanin (APC). These fluorochromes can be used simultaneously to stain and analyze the expression patterns of four different proteins in the same sample. The fluorochrome stained cell populations are analyzed using a FACSCalibur dual-laser flow cytometer. - [Read Characterization of Cells by Flow Cytometry Protocol]

Chlorophyll Content Protocol - http://homepages.gac.edu/~cellab/chpts/chpt8/ex8-2.html

Category: Plant Biology Protocols: Photosynthesis and Respiration Protocols

Protocol describing how to calculate chlorophyll content. - [Read Chlorophyll Content Protocol]

Clearing Arabidopsis Non-Ovule Materials With the HCG Solution Protocol - http://www.hybtech.org/Liu/HCG_clearing.htm

Category: Plant Biology Protocols

Protocol can be used for clearing intact non-ovule materials of arabidopsis, which can then be observed under Normarski optics. This is an efficient way to analyse root, seedling even flower development without sectioning. This protocol could also be used for clearing GUS stained material, after chlorophyll is removed by 70% ethanol. - [Read Clearing Arabidopsis Non-Ovule Materials With the HCG Solution Protocol]

Determination of Chlorophyll Content in Chloroplasts - http://www.bio.com/protocolstools/protocol.jhtml?id=p1285

Category: Plant Biology Protocols: Photosynthesis and Respiration Protocols

This protocol describes how to determine the chlorophyll content of chloroplasts using a spectrophotometric assay. - [Read Determination of Chlorophyll Content in Chloroplasts]

Fluorochrome Absorption and Emission Spectra - http://www.bdbiosciences.com/pharmingen/protocols/Fluorochrome_Absorption.shtml

Category: Molecular Imaging: Immunofluorescence Microscopy

Fluorochrome Absorption and Emission Spectra. BD Biosciences. Includes spectra of Allophycocyanin (APC), BD Cy-Chrome, Fluorescein isothiocyanate (FITC), R-phycoerythrin (R-PE), PE-Texas Redâ„¢, Peridinin chlorophyll protein (PerCP), PerCP-Cy5.5, APC-Cy7, Texas Redâ„¢. - [Read Fluorochrome Absorption and Emission Spectra]

Live-Cell Imaging of GFP in Plants - http://www.cshprotocols.org/cgi/content/full/2007/3/pdb.ip31

Category: Microscopy Protocols: Live-Cell Imaging Protocols

GFP serves as a molecular marker that can be imaged dynamically in living cells, both in its native form & as a fusion to other proteins. For GFP imaging, plants present the challenge of autofluorescence from chlorophyll, lignified cell walls, vacuolar contents, and other cell materials, all of which can obscure the GFP signal. Maximizing the signal-to-noise ratio is a major concern, and careful consideration should be given to the choice of tissue imaged, GFP expression level, etc. - [Read Live-Cell Imaging of GFP in Plants]