channels Protocols

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Flow Cytometry Calibration Protocols

Flow cytometers must be calibrated prior to fluorescence intensity measurements because of inherent instrument variability. To correct for this variability, a standard particle (fixed chicken red blood cells, or CRBCs) must be analyzed on the instrument prior to each experiment and photomultiplier tube (PMT) voltages adjusted accordingly to place the CRBC fluorescence emission peaks into predetermined target channels. - [Read Calibration of Becton Dickinson Flow Cytometers for Relative Fluorescence Intensity Measurements]

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

HUMAN EMBRYONIC STEM CELL CULTURE IN MICROFLUIDIC CHANNELS. Abhyankar et al., 2003. 7th lnternat~onal Conference on Miniaturized Chemical and Blochemlcal Analysts Systems - [Read HUMAN EMBRYONIC STEM CELL CULTURE IN MICROFLUIDIC CHANNELS]

Category: Antibody Protocols: Antibody Production Protocols

Polyclonal antibodies can be isolated from animal plasma or serum using the procedure described in this protocol. The Gradiflow BF400 instrument has two liquid streams that circulate through a separation cartridge positioned between two electrodes and composed of three hydrogel polyacrylamide membranes, which define the channels for the two sample streams. The central membrane forms a physical barrier between the two streams. - [Read Preparation of Polyclonal Antibodies from Plasma or Serum Using the Gradiflow BF400]

Category: Neuroscience Protocols: Patch Clamping Protocols

The technique of patch clamp recording was invented by Bert Sakmann and Erwin Neher in 1981 for which they received the NOBEL prize. The technique is best suited for the study of the behaviour of single ion channels, or macroscopic currents in small cells or macro-patches. The whole cell technique allows one to control the composition of solutes on both sides of the membrane. - [Read Whole Cell Patch Clamp Technique]