chain Protocols

Category: PCR Protocols: In-situ PCR Protocols

A protocol for application of Polymerase Chain Reaction (PCR) in situ. hybridization for the detection of hyphomycetes. Sterflinger et al 1998. - [Read A protocol for application of Polymerase Chain Reaction (PCR) in situ hybridization for detection of]

Category: PCR Protocols: AFLP PCR

Ulrich G. Mueller and L. LaReesa Wolfenbarger. Amplified fragment length polymorphisms (AFLPs) are polymerase chain reaction (PCR)-based markers for the rapid screening of genetic diversity. AFLP. TREE October 1999 - [Read AFLP genotyping and fingerprinting Review PDF]

Category: Fungi Protocols: Fungi Genetics Protocols

Standard operating procedure for the determination of tissue fungal burden utilizing quantitative real time polymerase chain reaction (QPCR). This standard operating procedure will provide information on how to assess fungal tissue burden of infected animals by use of a single copy (FKS) or multicopy gene (18s RNA) to assess the number of fungal cell nuclei present. - [Read Determination of Tissue Fungal Burden Utilizing Quantitative Real Time Polymerase Chain Reaction]

Category: PCR Protocols: PCR Optimization

Method for amplifying DNA enzymatically by the polymerase chain reaction (PCR), including procedures to quickly determine conditions for successful amplification of the sequence and primer sets of interest, and to optimize for specificity, sensitivity, and yield.  The first step of PCR simply entails mixing template DNA, two appropriate oligonucleotide primers, Taq or other thermostable DNA polymerases, deoxyribonucleoside triphosphates (dNTPs), and a buffer. - [Read Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization Protocol]

Category: PCR Protocols: In-situ PCR Protocols

In Situ Polymerase Chain Reaction: Past, Present, Future. Roche. - [Read In Situ Polymerase Chain Reaction Roche]

Category: Fungi Protocols: Fungi Genetics Protocols

Quick and reliable method to analyze meiotic segregation patterns in Coprinus cinereus using the polymerase chain reaction. The advantages of this method include: 1. The tissue is grown and lyophilized in the same tube, which facilitates the simultaneous analysis of many segregants. 2. Only one extraction step is necessary. 3. The markers are scored by gel electrophoresis, thereby bypassing Southern analysis. - [Read Method to Analyze Meiotic Segregation Patterns in Coprinus cinereus Using PCR]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

Quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Other PCR Procedures. - [Read Older RT-PCR and PCR Techniques Page.]

Category: PCR Protocols

Quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Other PCR Procedures. - [Read Older RT-PCR and PCR Techniques Page.]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: Standard PCR Protocol

Covers Almost Everything to do with Polymerase Chain Reaction - PCR Manual - Roche - [Read PCR Manual - Roche]

Category: PCR Protocols: Standard PCR Protocol

Covers Almost Everything to do with Polymerase Chain Reaction - PCR Manual - Roche - [Read PCR Manual - Roche]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: PCR Optimization Protocols

PCR Tips and Tricks - General Guidelines and Troubleshooting PCR polymerase chain reaction. The Genomic Variation Laboratory, U.C Davis - [Read PCR Tips and Tricks]

Category: PCR Protocols: PCR Optimization

PCR Tips and Tricks - General Guidelines and Troubleshooting PCR polymerase chain reaction. The Genomic Variation Laboratory, U.C Davis - [Read PCR Tips and Tricks]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: Standard PCR Protocol

Polymerase Chain Reaction (PCR) Lecture - Properties and Choices of Polymerase, Basic Protocol for PCR. Dr. Michael Blaber - [Read Polymerase Chain Reaction (PCR) Lecture]

Category: PCR Protocols: Standard PCR Protocol

Polymerase Chain Reaction (PCR) Lecture - Properties and Choices of Polymerase, Basic Protocol for PCR. Dr. Michael Blaber - [Read Polymerase Chain Reaction (PCR) Lecture]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: PCR Optimization Protocols

Optimizing PCR Reaction Conditions. Optimizing PCR Tips and Guide. University of Alaska Museum - [Read Polymerase Chain Reaction (PCR) Optimization]

Category: DNA Protocols: DNA Sequencing Protocols: Dideoxy Mediated Sequencing Protocols

Protocol describes how double-stranded plasmid DNA is denatured by alkali and annealed to an appropriate oligonucleotide primer, in preparation for dideoxy-sequencing reactions catalyzed by Sequenase. - [Read Preparing Denatured Templates for Sequencing by Dideoxy-mediated Chain Termination Protocol]

Category: PCR Protocols: Quantitative PCR Protocols

Protocol that uses the polymerase chain reaction (PCR) to quantitate the numbers of a particular DNA sequence, from 1 to 20,000 molecules per sample.  In addition, it helps assess the presence of contaminating sequences, which can seriously affect the outcome of the procedure. - [Read Quantitation of Rare DNAs by PCR Protocol]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: Standard PCR Protocol

Quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Other PCR Procedures. Informative Guide. Jack Vanden Heuvel. - [Read Quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Other PCR Procedures]

Category: PCR Protocols: Standard PCR Protocol

Quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Other PCR Procedures. Informative Guide. Jack Vanden Heuvel. - [Read Quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Other PCR Procedures]

Category: Nucleic Acid Modification Protocols: Nucleic Acid Radiolabeling Protocols

In this protocol, double-stranded DNA probes, labeled in each strand, are produced in conventional PCRs containing equal concentrations of two primers, a double-stranded DNA template, three unlabeled dNTPs at concentrations exceeding the Km, and one [{alpha}-32P]dNTP at a concentration at or slightly above the Km (2-3 µm) for a thermostable DNA polymerase such as Taq. - [Read Radiolabeling of DNA Probes by the Polymerase Chain Reaction Protocol]

Category: PCR Protocols

Protocol describes how double-stranded DNA probes, labeled in each strand, are produced in conventional PCRs containing equal concentrations of two primers, a double-stranded DNA template, three unlabeled dNTPs at concentrations exceeding the Km, and one [{alpha}-32P]dNTP at a concentration at or slightly above the Km (2-3 µm) for a thermostable DNA polymerase such as Taq. - [Read Radiolabeling of DNA Probes by the Polymerase Chain Reaction Protocol]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: Standard PCR Protocol

Standard Polymerase Chain Reaction PCR Protocol. (Dr. Chen, U.College. - [Read Standard Polymerase Chain Reaction]

Category: PCR Protocols: Standard PCR Protocol

Standard Polymerase Chain Reaction PCR Protocol. (Dr. Chen, U.College. - [Read Standard Polymerase Chain Reaction]

Category: PCR Protocols: Standard PCR Protocol

Protocol describes how to amplify a segment of double-stranded DNA in a chain reaction catalyzed by a thermostable DNA polymerase.  It is the foundation for all subsequent variations of the polymerase chain reaction. - [Read The Basic Polymerase Chain Reaction Protocol]

Category: Molecular Model Organisms: Mouse Animal Protocols: Mouse Genotyping Protocols

Universal Mouse Genotyping Protocol (downloadable pdf click here). This protocol is designed to detect sequences in the murine genome by polymerase chain ... - [Read Universal Mouse Genotyping Protocol]