cftr Protocols

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol outlines the general procedure and requirements for in vitro translation of CFTR and outlines some assays using in vitro translated product. Core glycosylation of CFTR occurs in the ER. An assay for this processing step requires the addition of microsomal membranes to the basic in vitro translation mixture. This protocol takes this into account. - [Read In vitro Translation Assays for CFTR]

Category: Cell Biology and Cell Culture: Epithelial Cell Culture

Protocol for the Isolation of Pancreatic Duct Epithelial Cells. Gray and Argent. - [Read Protocol for the Isolation of Pancreatic Duct Epithelial Cells]

Category: Electrophysiology Ion Channels

The Whole-Cell Patch-Clamp Technique – a powerful tool to approach CFTR function. Martin J. Hug. University of Muenster, Institute of Physiology. Germany - [Read The Whole-Cell Patch-Clamp Technique – a powerful tool to approach CFTR function]