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Centrifuge Article and Tips - http://abacus.bates.edu/~ganderso/biology/resources/centrifugation.html

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Balancing Rotors, Tips on Centrifuging. Cautions for centrifuge usage. - [Read Centrifuge Article and Tips]

Centrifuge Safety - http://www.chm.bris.ac.uk/safety/centrifg.htm

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Hazards of Centrifuge use in the laboratory and Operation. Rotor care, and Pre-run safety checks for rotors and centrifuges. - [Read Centrifuge Safety]

Centrifuge Safety - Great Tips - http://www.crscientific.com/centrifuge-safety.html

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Balancing the tubes in the rotor, labeling tubes, and other safety concerns for centrifuge safety. CR Scientific. - [Read Centrifuge Safety - Great Tips]

Centrifuge Safety - High Speed Hazards - http://www.ehs.wsu.edu/Factsheets/FAQCentrifuge.asp

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Centrifuge Safety - High Speed Hazards. Safe Centrifuge Use , Care and Maintenance, and maintaining a log book for centrifuges. EHS Washington state Univ. - [Read Centrifuge Safety - High Speed Hazards]

Centrifuge Tube Chemical Resistance Chart - http://www.bdbiosciences.com/discovery_labware/technical_resources/labtools/p38.pdf

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Centrifuge Tube Chemical Resistance Chart. Polypropylene, polystyrene. PS, PP. BD Biosciences. - [Read Centrifuge Tube Chemical Resistance Chart]

Excellent Centrifuge Safety Use Manual PDF - http://www.hse.ubc.ca/resource/files/CentrifugeSafetyManual.pdf

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Excellent Centrifuge Safety Use Manual. 30 pages. Training, Use, Maintenance, Records and Responsibility, Mechanical Breakdown, Emergency Procedure, Specific Safety Instructions Beckman Sorvall and Eppendorf. HSE UBC. - [Read Excellent Centrifuge Safety Use Manual PDF]

Articles (1-10 of 15)

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

Probe Preparation for 22 x 40 mm DNA Microarrays

Probe Preparation for 22 x 40 mm DNA Microarrays Protocol.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.