User Panel

My Panel

Bookmark Science Articles

center Protocols

Search results for: center

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

A Method for Micro-Scale Isolation and Purification of Gangliosides - http://www.glycotech.com/protocols/Proto1.html

Category: Molecular Biology Protocols: Carbohydrate Protocols

Solvent partition protocol allows the isolation of gangliosides from small samples and from samples where ganglioside concentrations are low, especially relative to the concentration of potentially contaminating proteins and other large molecular weight species. Stephan Ladisch Director, Center for Cancer and Transplant Biology, Children's National Medical Center, Washington, D.C. - [Read A Method for Micro-Scale Isolation and Purification of Gangliosides]

Aggregation: Production of Chimerae - http://transgenics.ufscc.ufl.edu/TCF05agg_instruct.html

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: ES Cell Aggregations - Production of Chimerae

Aggregation: Production of Chimerae. Detailed ES cell growth and preparation information for aggregations. Edward W. Scott Univ. Florida Shands Cancer Center - [Read Aggregation: Production of Chimerae]

Bisulfite Treatment of DNA for Methylation Analysis - http://www.mdanderson.org/departments/methylation/display.cfm?id=955D5740-37D4-444F-ACF0193F8EC3989B&method=displayFull

Category: Epigenetics and Methylation Protocols: Bisulfite Treatment Methylation Assay Protocol

Adapted from Frommer et.al. Good protocol for bisulfite treatment of DNA. Includes tips on Methylation PCR for CpG methylation analysis. University of Texas M. D. Anderson Cancer Center - [Read Bisulfite Treatment of DNA for Methylation Analysis]

cDNA Library Screening Protocol - ZAPII - http://www.people.virginia.edu/~gwl6s/protocols/cdna.html

Category: RNA Protocols: cDNA Libraries Library

cDNA Library Screening Protocol - ZAPII Detailed Protocol. XLI-Blue MRF' and cDNA phage library screening. Dr. Laurie. University of Virginia Health Science Center - [Read cDNA Library Screening Protocol - ZAPII]

Coomasie and SYPRO Destaining Method - http://www.cbs.umn.edu/msp/protocols/destaining.shtml

Category: Proteomic Protocols

Coomasie and SYPRO Destaining Method Protocol. MSCLS Center for Mass Spectrometry and Proteomics - [Read Coomasie and SYPRO Destaining Method]

Deglycosylation of Glycoproteins Using Endoglycosidases - http://www.glycotech.com/protocols/Proto3.html

Category: Molecular Biology Protocols: Carbohydrate Protocols

Deglycosylation steps are different with every glycoprotein and must be determined empirically (Methods in Enzymology, 1994, 230: 44-57). A typical test reaction of human transferrin is described. T.H. Plummer, Jr. and A.L. Tarentino, Department of Biochemistry, New York State Department of Health, Wadsworth Center for Laboratories and Research, Albany, New York - [Read Deglycosylation of Glycoproteins Using Endoglycosidases]

Deltavision Deconvolution Instructions - http://depts.washington.edu/keck/dv/start.html

Category: Molecular Imaging

Deltavision Deconvolution Instructions. Keck Imaging Center. - [Read Deltavision Deconvolution Instructions]

Detection of Alu by PCR - http://www.accessexcellence.com/AE/AEPC/DNA/detection.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

Detection of Alu by PCR - A Human DNA Fingerprinting Lab Protocol - 1994 Cold Spring Harbor Laboratory DNA Learning Center - [Read Detection of Alu by PCR]

Detection of Alu by PCR - http://www.accessexcellence.com/AE/AEPC/DNA/detection.html

Category: PCR Protocols

Detection of Alu by PCR - A Human DNA Fingerprinting Lab Protocol - 1994 Cold Spring Harbor Laboratory DNA Learning Center - [Read Detection of Alu by PCR]

dsRNA Synthesis Protocol - http://flyrnai.org/RNAi_FAQ_dsrna_syn.html

Category: siRNA and RNAi Protocols

dsRNA From Clones, Primer Designed dsRNA. Drosophila RNAi Screening Center at Harvard Medical School - [Read dsRNA Synthesis Protocol]

How to Deposit Materials in the Fungal Genetics Stock Center (FGSC) - http://www.fgsc.net/neurosporaprotocols/How%20to%20deposit%20materials%20final.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to deposit materials in the Fungal Genetics Stock Center (FGSC). - [Read How to Deposit Materials in the Fungal Genetics Stock Center (FGSC)]

Image Processing Tools - http://depts.washington.edu/keck/leica/ip.html

Category: Molecular Imaging

Image Processing Tools. Keck Imaging Center - [Read Image Processing Tools]

in vitro Transcription Protocol - http://penguin.uchc.edu/~intron/Protocols/in%20vitro%20transcription.html

Category: RNA Protocols: In Vitro Transcription T7 SP6 T3 Protocols

Short and simple In Vitro Transcription Protocol. Brent Graveley, University of Connecticut Health Center. - [Read in vitro Transcription Protocol]

Leica Confocal Instructions - http://depts.washington.edu/keck/leica/instructions.htm

Category: Molecular Imaging: Confocal Microscopy Protocols and Information

Leica Confocal Instructions. Keck Imaging Center. - [Read Leica Confocal Instructions]

Leica SL Confocal Microscopy Imaging Instructions - http://depts.washington.edu/keck/LeicaSL/SL%20Instructions.htm

Category: Molecular Imaging: Confocal Microscopy Protocols and Information

Leica SL Confocal Microscopy Imaging Instructions. Keck Imaging Center. - [Read Leica SL Confocal Microscopy Imaging Instructions]

Light Microscopy Techniques - http://www.hei.org/research/aemi/clm.htm

Category: Molecular Imaging: Light Microscopy

Ahmanson Center for Advanced Electron Microscopy and Imaging. Use of Semi-Thin Cryosections for Light Microscopy, Immunolabeling of Cryosections on Glass Slides, Problems with Autofluorescence. House Ear Institute - [Read Light Microscopy Techniques]

List of standard EM Center procedures/protocols - http://anatomy.iupui.edu/emcenter/protocols.html

Category: Molecular Imaging: Electron Microscopy Protocols

Protocol for sectioning is on a separate page. 2. Standard Procedure To Section for Transmission Electron Microscopy, EM Center, Indiana University School ... List of standard EM Center procedures/protocols - [Read List of standard EM Center procedures/protocols]

M9 Plate Supplementation Table - http://www.fhcrc.org/science/labs/gottschling/bac/bsup.html

Category: Microbiology: Bacterial E.Coli Culture Media & Plates

M9 Plate Supplementation Table. URA, LEU, MET, HIS, TRP. Fred Hutchinson Cancer Research Center - [Read M9 Plate Supplementation Table]

Millipore ZipTip detailed protocol. MSCLS - http://www.cbs.umn.edu/msp/protocols/ziptip.shtml

Category: Proteomic Protocols

ZipTip Protocol detailed protocol. MSCLS Center for Mass Spectrometry and Proteomics - [Read Millipore ZipTip detailed protocol. MSCLS]

PCR Genotyping of Mutant Strains - http://www.fhcrc.org/science/labs/soriano/protocols/pcrgen.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

PCR Genotyping of Mutant Strains. Fred Hutchinson Cancer Research Center - [Read PCR Genotyping of Mutant Strains]

PCR Genotyping of Mutant Strains - http://www.fhcrc.org/science/labs/soriano/protocols/pcrgen.html

Category: PCR Protocols

PCR Genotyping of Mutant Strains. Fred Hutchinson Cancer Research Center - [Read PCR Genotyping of Mutant Strains]

PCR Protocol Buffer Creation - http://www.fhcrc.org/science/labs/soriano/protocols/pcr.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

PCR Protocol Buffer Creation. Based on Jane Gitschier's group.Fred Hutchinson Cancer Research Center. - [Read PCR Protocol Buffer Creation]

PCR Protocol Buffer Creation - http://www.fhcrc.org/science/labs/soriano/protocols/pcr.html

Category: PCR Protocols

PCR Protocol Buffer Creation. Based on Jane Gitschier's group.Fred Hutchinson Cancer Research Center. - [Read PCR Protocol Buffer Creation]

PCR Screens of ES Cell Clones - http://www.fhcrc.org/science/labs/soriano/protocols/pcres.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

Identification of targeted ES cell clones by PCR. Fred Hutchinson Cancer Research Center. - [Read PCR Screens of ES Cell Clones]

PCR Screens of ES Cell Clones - http://www.fhcrc.org/science/labs/soriano/protocols/pcres.html

Category: PCR Protocols

Identification of targeted ES cell clones by PCR. Fred Hutchinson Cancer Research Center. - [Read PCR Screens of ES Cell Clones]

Articles (1-1 of 1)

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

Items 1-25 out of 37 displayed.

First [1] 2 Next Last

Total records: 37