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Dissociated Retinal Cell Scoring Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/score.html

Category: Immunohistochemistry Protocols

Protocol for dissociated retinal cell scoring. Includes: Rods; Cones; Bipolars; Müller Glial Cells; Pan-Amacrine/Horizontal. - [Read Dissociated Retinal Cell Scoring Protocol]

Dissociation-and-Reaggregation of Animal Cap Cells Protocol - http://www.hhmi.ucla.edu/derobertis/protocol_page/Pdfs/Dissociated.pdf

Category: Xenopus Protocols: Xenopus Embryo Manipulation Protocols

Protocol for dissociation-and-reaggregation of animal cap cells. - [Read Dissociation-and-Reaggregation of Animal Cap Cells Protocol]

DNA Content using Hoechst 33342 in Unfixed Cells Protocol - http://depts.washington.edu/immunweb/cellanalysis/HoeDNA.html

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: DNA Staining Protocols for Flow Cytometry

Protocol for DNA content using Hoechst 33342 in unfixed cells. - [Read DNA Content using Hoechst 33342 in Unfixed Cells Protocol]

DNA Extraction from Cheek Cells - http://geneticslab.topcities.com/cheekcells.htm

Category: DNA Protocols: DNA Extraction Protocols

Cheek cells obtained by rinsing the mouth commercial mouth wash solution. Mouth wash is then discarded into a sterile conical tube and sent to the lab. Based on Salting out procedure. DNA Laboratory, Medical School, Malta. - [Read DNA Extraction from Cheek Cells]

DNA Isolation from Onion Cells - http://www.accessexcellence.org/AE/AEC/AEF/1994/dollard_onionDNA.html

Category: DNA Protocols: DNA Extraction Protocols

Allows students to easily isolate chromosomal DNA from onion cells using the same basic tools and methods that scientists use in the lab. A good introduction to using pipettes. Kate Dollard - [Read DNA Isolation from Onion Cells]

DNA laddering assay for treated cells - http://www.celldeath.de/apometh/dnaladd.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols

DNA laddering can be detected from samples with only 8% apoptotic cells. Alternatively, the cells can be stained with DAPI and analysed by flow cytometry. CellDeath.de - [Read DNA laddering assay for treated cells]

DNA Preparation and Needle Loading for Gene Delivery by Microinjection Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4652

Category: Microinjection Protocols: Microinjection of DNA Protocols

For microinjection into cells, high-quality plasmid DNA is purified using standard procedures. The resulting preparation is then delivered into the microinjection needle by either a back-filling or a forward-filling approach. - [Read DNA Preparation and Needle Loading for Gene Delivery by Microinjection Protocol]

DNA Preparation from Lymphoblastoid Cells - http://www.riedlab.nci.nih.gov/publications/2440.2744_DNA_Prep_Lymphoblast.pdf

Category: DNA Protocols: DNA Extraction Blood

Protocol for the DNA Preparation from Lymphoblastoid Cells. Thomas Ried Lab, NCI. - [Read DNA Preparation from Lymphoblastoid Cells]

DNA Transfection by Biolistics Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3874

Category: Gene Delivery Protocols: Biolistics Protocols

In this protocol, gold or tungsten particles are coated with DNA and then shot from a gun into monolayers of mammalian cells. - [Read DNA Transfection by Biolistics Protocol]

DNA Transfection by Electroporation - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3751

Category: Transfection Protocols: Stable Transfection Protocols

Pulsed electrical fields can be used to introduce DNA into a wide variety of animal cells. Electroporation works well with cell lines that are refractive to other techniques, such as calcium phosphate-DNA coprecipitation. But, as with other transfection methods, the optimal conditions for electroporating DNA into untested cell lines must be determined experimentally. - [Read DNA Transfection by Electroporation]

DNA Transfection Mediated by Lipofection Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3870

Category: Transfection Protocols

Protocol should be viewed as a starting point for systematic optimization of transfection mediated by lipofecting agents. Once a positive signal has been obtained from a transfected plasmid carrying a standard reporter gene, optimal conditions for transfection can be established by systematic variation of parameters such as the initial cell density, the amount and purity of DNA, the media and serum, and the time of exposure of the cells to the cationic-lipid-DNA complex. - [Read DNA Transfection Mediated by Lipofection Protocol]

DNA Transfection Using Polybrene Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3875

Category: Transfection Protocols

Polybrene and DMSO can be used to achieve stable transformation of several types of cells by plasmid DNA. The yield of transformants is up to 15-fold greater with Polybrene than with calcium phosphate-DNA coprecipitation. However, there is no difference between the two methods in the efficiency of transformation of cells by high-molecular-weight DNA. - [Read DNA Transfection Using Polybrene Protocol]

DNA-Methyltransferase Assay Protocol - http://www.mdanderson.org/departments/methylation/display.cfm?id=9B5B6082-C53A-4A86-BEE3EE149A52251E&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: Nucleic Acid Modification Protocols: DNA Methylation Protocols

Protocol for DNA-methyltransferase assay. Includes: Homogenize Cells/Tissues; Quantitate Protein Concentration; Dilute Samples; The Assay Proper; Troubleshooting. - [Read DNA-Methyltransferase Assay Protocol]

Drosophila Medium - http://flyrnai.org/RNAi_FAQ_dros_cc.html#I.

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila medium. Includes: S2, S2C, S2*, S2R+, S3, l(2)mbn, Kc(167), & DL2 cells; ML-DmBG2 & ML-DmBG6 cells; clone8 (CL8) cells. - [Read Drosophila Medium]

Dust Toxicity in Rat Alveolar Macrophage Cultures Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/32.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cytotoxicity Assays in Cell Culture Protocols

Macrophage cells in culture may be exposed to particulate matter, and resultant effects upon cell viability determined by vital dye exclusion and enzyme leakage assays. - [Read Dust Toxicity in Rat Alveolar Macrophage Cultures Protocol]

Ecdysone as Regulator of Inducible Gene Expression in Mammalian Cells - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3186

Category: Reporter Gene Assays

Protocol describing ecdysone as regulator of inducible gene expression in mammalian cells. - [Read Ecdysone as Regulator of Inducible Gene Expression in Mammalian Cells]

Electro Competent Cells Protocol - http://hulab.tamu.edu/wiki/index.php/Electro_competent_cells

Category: E Coli Protocols: E coli Transformation Protocols

Protocol for electro competent cells. - [Read Electro Competent Cells Protocol]

Electro Competent Cells Protocol - http://hulab.tamu.edu/wiki/index.php/Electro_competent_cells

Category: Competent Cells Protocols

Protocol for electro competent cells. Preparation of Electro competent E. coli cells (per the BioRad directions). - [Read Electro Competent Cells Protocol]

Electron Microscopy of the Cytoskeleton of Cultured Cells - http://www.borisylab.northwestern.edu/pages/protocols/electmicrosctext.html

Category: Molecular Imaging: Electron Microscopy Protocols

The protocol for immunolabeling for electron microscopy depends mostly on the primary antibody and its ability to recognize antigen under particular ...Electron Microscopy of the Cytoskeleton of Cultured Cells. Boris Lab. - [Read Electron Microscopy of the Cytoskeleton of Cultured Cells]

Electron Microscopy of the Cytoskeleton of Cultured Cells - http://www.borisylab.northwestern.edu/pages/protocols/electmicrosc.html

Category: Microscopy Protocols: Electron Microscopy Protocols

Article describe the preparation of cells for correlative electron microscopy after live light microscopic observation of fluorescently labeled cytoskeletal proteins microinjected into the same cells. Since identification of cytoskeletal elements in electron microscopic preparations is an essential part of any correlative study, procedures for immunogold labeling of cytoskeletal components and for myosin S1 decoration of actin filaments are also described. - [Read Electron Microscopy of the Cytoskeleton of Cultured Cells]

Electron Microscopy Protocols - http://www.emlab.ubc.ca/p_em.htm

Category: Molecular Imaging: Electron Microscopy Protocols

Electron Microscopy Protocols ... Chemical Fixation Protocol for Suspension-Cultured Plant Cells for TEM; Standard Glutaraldehyde Fixation for TEM of Animal ...Electron Microscopy Protocols. UBC BioImaging Facility - Bio-Rad Radiance Plus Confocal Microscope - Starting Up, page 1 - [Read Electron Microscopy Protocols]

Electron Microscopy Protocols Yale - http://cellserv.med.yale.edu/imaging/ccmi/elect_protocols.html

Category: Molecular Imaging: Electron Microscopy Protocols

Protocol 2 - Embedding of cell monolayer in epoxy resin. Protocol 3 - Embedding of scraped cells ... Welcome to the new CCMI: Electron Microscopy Website. ... Yale Protocols. - [Read Electron Microscopy Protocols Yale]

Electroporating DNA into Embryonic Stem (ES) Cells and Selection Methods Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4409

Category: Mouse Protocols: Mouse Genetics Protocols

Protocol describes a method for electroporating DNA into ES cells, as well as selection methods. Pilot studies should be performed to optimize the conditions for each DNA construct. The selection method described here is one of the most complex. It involves targeting constructs in which the bacterial neomycin-resistance gene disrupts the coding sequence of the mouse gene. - [Read Electroporating DNA into Embryonic Stem (ES) Cells and Selection Methods Protocol]

Electroporation of Cell Lines With DNA - http://users.path.ox.ac.uk/~ciu/Methods/DNA/electroporation.html

Category: Cell Biology and Cell Culture: Cell Electroporation Protocols

Electroporation of Cell Lines With DNA. Electroporation for the efficient transfection of mammalian cells with DNA. Chu et al. Cellular Immunology Oxford - [Read Electroporation of Cell Lines With DNA]

Electroporation of Cells a FAQs - http://www.eppendorfna.com/faqs/CellBiology.asp#electroporation

Category: Cell Biology and Cell Culture: Cell Electroporation Protocols

Electroporation of Cells a FAQs. Eppendorf. - [Read Electroporation of Cells a FAQs]

Articles (1-10 of 24)

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

Microinjection Pipettes Protocol

Microinjection Pipettes Preparation Protocol.

Acid Washing Microinjection Pipettes Protocol

Acid Washing of Glass microinjection pipettes protocol.

Paraffin Embedding Protocol

Paraffin Embedding Protocol for molecular profiling. This Paraffin Embedding Protocol describes the processing of the tissues into sections following ethanol fixation. Molecular profiling (MP) is a technique that is used to visualize the global patterns of RNA expression or protein expression in various cell types and disease processes.

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