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Apoptosis Protocols - http://www.celldeath.de/apometh/apometh.htm

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Apoptosis Protocols. Listings. Celldeath.de - [Read Apoptosis Protocols]

Culture of Human Prostatic Carcinoma Cell Lines - http://www.celldeath.de/apometh/rpmi.html

Category: Cell Biology and Cell Culture: Cell Line Culture Conditions

Culture of Human Prostatic Carcinoma Cell Lines, Growing and splitting the cells, Preparation of frozen stocks in liquid nitrogen, How to bring frozen cells back into culture, Concentrations of antibiotics for the selection of stable transfectants. LNCa - [Read Culture of Human Prostatic Carcinoma Cell Lines]

Detailed NF-kB EMSA Protocol - http://www.celldeath.de/apometh/emsa.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols: NF-kB EMSA Protocols

Detailed NF-kB EMSA Protocol. Includes Nuclear extract preparation, Labeling of the oligonucleotide probe: T4 Polynucleotide Kinase Reaction, Supershift assay to identify NF-kB, and recipes for EMSA buffers. Celldeath.de - [Read Detailed NF-kB EMSA Protocol]

DNA laddering assay for treated cells - http://www.celldeath.de/apometh/dnaladd.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols

DNA laddering can be detected from samples with only 8% apoptotic cells. Alternatively, the cells can be stained with DAPI and analysed by flow cytometry. CellDeath.de - [Read DNA laddering assay for treated cells]

Electrophoretic Mobility Shift Assay (EMSA) for the detection of nuclear NF-kB - http://www.celldeath.de/apometh/emsa.html

Category: DNA Protocols: EMSA DNA-Protein Protocols

Electrophoretic Mobility Shift Assay (EMSA) for the detection of nuclear NF-kB. CellDeath.de - [Read Electrophoretic Mobility Shift Assay (EMSA) for the detection of nuclear NF-kB]

Gentle lysis of cells in sucrose-containing Mito-Buffer - http://www.celldeath.de/apometh/mitobuff.html

Category: Cell Biology and Cell Culture: Cell Lysis Protocols

Gentle lysis of mammalian cells for cytochrome c release assay. Based on Yang et al., 1997 Celldeath.de - [Read Gentle lysis of cells in sucrose-containing Mito-Buffer]

Gentle lysis of mammalian cells for cytochrome c release assay - http://www.celldeath.de/apometh/mitobuff.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Lysis in the sucrose-containing Buffer ("Mito-Buffer") is supposed to prevent accidential disrupture of the mitochondria to prevent the leakage of mitochondrial proteins (such as cytochrome c) into the cytosol. Celldeath.de - [Read Gentle lysis of mammalian cells for cytochrome c release assay]

Radioactive DNA Fragmentation Assay - http://www.celldeath.de/apometh/dnafragm.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols: DNA Fragmentation Assay

DNA Fragmentation Assay allows determination of the amount of DNA that is degraded upon treatment of cells with treatment of apoptotic agents. Celldeath.de - [Read Radioactive DNA Fragmentation Assay]