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Automated CellTiter-Glo Luminescent Cell Viability Assay Protocol - http://www.promega.com/tbs/ep014/ep014.pdf

Category: Cell Biology and Cell Culture: Cell Viability Protocols

Protocol for the automated cell titer- glo luminescent cell viability assay. - [Read Automated CellTiter-Glo Luminescent Cell Viability Assay Protocol]

B- Galactosidase Assay Protocol - http://www.biochem.northwestern.edu/ibis/morimoto/research/Protocols/VI.%20Transcription/I.%20b-gal%20.pdf

Category: Reporter Gene Assays: B-Galactosidase Assay Protocols

The recommended amount of RSV-ß-Galactosidase plasmid to use for transfection of cells (60 mm or 100 mm dish) is 1-2 µg. The optimal amount of plasmid DNA will be determined by the efficiency of transfection , which is very dependent upon the particular cell line and transfection protocol. - [Read B- Galactosidase Assay Protocol]

Bacterial Cell Maintenance Protocol - http://www.genome.ou.edu/protocol_book/protocol_partI.html#I.G

Category: Bacterial Cell Culture Protocols: Bacterial Stocks Protocols

4 strains of E. coli are used in these studies: JM101 for M13 infection and isolation, XL1BMRF'for M13 or pUC-based DNA transformation, and ED8767 for cosmid DNA transformation. To maintain their respective F' episomes necessary for M13 viral infection, JM101 is streaked onto a M9 minimal media plate and XL1BMRF' is streaked onto an LB plate containing tetracycline. ED8767 is streaked onto an LB plate. These plates are incubated at 37degC overnight. For each strain, 3 ml. of appropriate liquid.. - [Read Bacterial Cell Maintenance Protocol]

Baculovirally Infected Sf9 Cell Lysis Protocol - http://ruummc.med.uu.nl/subsite/RepHTML/Protocols/Sf9lysis.html

Category: Cloning Protocols: Baculovirus Protein Expression Protocols

Protocol for Baculovirally infected Sf9 cell lysis. Includes: Harvesting; Lysis. - [Read Baculovirally Infected Sf9 Cell Lysis Protocol]

Basic Cell Culture Protocols - http://www.hyclone.com/library/basicprotocols.htm

Category: Cell Biology and Cell Culture

Basic Cell Culture Protocols. Passaging, Counting, Viability and more. Hyclone - [Read Basic Cell Culture Protocols]

Basic Techniques for Mammalian Cell Tissue Culture Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662B5FE053DEE8CB4AC3EB6F729A11&objectid=667396C6F0D96C40EB68A3D8DB8B201A

Category: Cell Culture Protocols: Sterile Technique Protocols

Cultured mammalian cells are used extensively in cell biology studies; it requires a number of special skills in order to be able to preserve the structure, function, behavior and biology of the cells. This unit describes the basic skills required to maintain and preserve cell cultures: aseptic technique, medium characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells. - [Read Basic Techniques for Mammalian Cell Tissue Culture Protocol]

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

Bead Aggregation Assay Protocol - http://www.natureprotocols.com/2006/09/26/bead_aggregation_assay_to_demo.php

Category: Neuroscience Protocols

Bead aggregation assay to demonstrate presynaptic differentiation induced by the NGL family of cell adhesion molecules. - [Read Bead Aggregation Assay Protocol]

BHK-21. Basic Cell Culture Protocols - http://www.sci.sdsu.edu/classes/chemistry/chem467l/mardahl/basiccell.html

Category: Cell Biology and Cell Culture

Basic Cell Culture Protocols. BHK-21. - [Read BHK-21. Basic Cell Culture Protocols]

Biochemical Analysis Of Cell Death Using Colorimetric Quantification Of Caspase Activation (Subscrip - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4435

Category: Cell Biology and Cell Culture: Apoptosis Protocols: Apoptosis Analysis

Biochemical Analysis Of Cell Death Using Colorimetric Quantification Of Caspase Activation (Subscription Required). Biochemical Analysis of Cell Death Using Colorimetric Quantification of Caspase Activation Shailaja Kasibhatla et al., - [Read Biochemical Analysis Of Cell Death Using Colorimetric Quantification Of Caspase Activation (Subscrip]

Breaking the Tips of Embryonic Stem (ES) Cell Injection Needles Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4417

Category: Stem Cell Protocols: Stem Cell Injection Protocols

Injection needles require sharp tips that allow the blastocyst to be penetrated easily. This protocol describes a method of creating sharp injection needle tips. - [Read Breaking the Tips of Embryonic Stem (ES) Cell Injection Needles Protocol]

C25 Isolation from spleen, thymus, pancreas, alveolar tissue and other tissues - http://www.axis-shield.com/densityhome/optiprep/C25.pdf

Category: Cell Biology and Cell Culture

OptiPrep Application sheet C14 describes procedures for determining the density and sedimenting properties of any cell (of any size or density) using either a continuous or discontinuous gradient of iodixanol. This Application Sheet describes procedures aimed at isolating specifically a relatively low-density cell fraction from any tissue. - [Read C25 Isolation from spleen, thymus, pancreas, alveolar tissue and other tissues]

CaCl2 Competent Cell Stock Protocol - http://www.bio.indiana.edu/~chenlab/potocols/CompetentCellStock.htm

Category: Microbiology: Bacterial Competent Cells Preparation E.Coli

Simple Protocol for CaCl2 Competent Cells. Chen Lab. Indiana University Bloomington - [Read CaCl2 Competent Cell Stock Protocol]

Calcium Flux Analysis Protocol for Flow Cytometry - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/calcium.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol for calcium flux analysis. Includes: Reagent Preparation; Sample Preparation; Cell Surface Staining; Instrument Set up; Optimizing the Instrument Settings; Recording Data to Apply Compensation; Recording Experimental Data. - [Read Calcium Flux Analysis Protocol for Flow Cytometry]

Calcium phosphate Tranfection for MDCK cells - http://www.its.caltech.edu/%7Ebjorker/Ca-P_Transfection_MDCK.pdf

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

Protocol for cell transfection, clone screening and picking cell clones. Bjorkman Group. California I.T - [Read Calcium phosphate Tranfection for MDCK cells]

Calcium Phosphate Transfection Method - http://www.flemingtonlab.com/Protocols/CalciumPhosphateTransf.pdf

Category: Transfection Protocols: Calcium Phosphate Transfection Protocols

This calcium phosphate transfection method works best in cell lines that are 1) highly transformed and 2) adherent (Hela, U2OS, SAOS2, AdAH, NPC-KT and obtain from 20% to 100% transfection efficiency depending on the cell line). Works well for transient experiments but precautions should be used in the design and interpretation of experiments based on the discussion below. Also works very well for generating stable cell lines. This method is quite sensitive to the amount of input plasmid. - [Read Calcium Phosphate Transfection Method]

Calcium Phosphate Transfection of Neurons in Primary Culture - http://gweb1.ucsf.edu/labs/finkbeiner/Protocol/protocol_list/Ca_P_TX_R.shtml

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

Detailed Neuronal cell transfection protocol and methods. Steven Finkbeiner. UCSF. - [Read Calcium Phosphate Transfection of Neurons in Primary Culture]

Calcium-phosphate-mediated Transfection of Eukaryotic Cells with Plasmid DNAs - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3871

Category: Transfection Protocols: Calcium Phosphate Transfection Protocols

Calcium phosphate forms an insoluble precipitate with DNA, which attaches to the cell surface and is taken into the cells by endocytosis. The protocol is easily adapted for use with other types of cells, both adherent and nonadherent. This protocol is a modified version of a method published by Jordan et al. (1996) who rigorously optimized calcium-phosphate-based transfection methods for Chinese hamster ovary cells and the 293 line of human embryonic kidney cells. - [Read Calcium-phosphate-mediated Transfection of Eukaryotic Cells with Plasmid DNAs]

Calibration of Micropipette Tips Protocols - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4655

Category: Microinjection Protocols: Microinjection Equipment

This protocol describes an easy method for calibrating micropipette tips that have been pulled in the laboratory. It is essential to estimate the internal diameter of the pulled micropipette tip when adjusting parameters for a new puller or new type of glass tubing. A tip diameter of ~0.3 µm is optimal for the microinjection of mammalian cells in culture (e.g., CHO, PtK1, and COS-7). A 10% increase in diameter increases the delivery rate by more than 30% and can cause cell damage. - [Read Calibration of Micropipette Tips Protocols]

CaPO4 Transfection for Chromaffin Cells - http://medicine.ucsd.edu/hypertension/list_of_protocols/15_CaPO4_Transfect_sw.htm

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

Transfection of DNA into cells using PIBS, DNA, CaCl2 and Calcium phosphate. Daniel O'Connor, UCSD Chromaffin Cell and Hypertension Research - [Read CaPO4 Transfection for Chromaffin Cells]

Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates - http://www.glycotech.com/protocols/Proto9.html

Category: Molecular Biology Protocols: Carbohydrate Protocols

Protocol detects specific cell adhesion to glycolipids resolved on TLC plates. Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates. Ronald L. Schnaar~Professor, Johns Hopkins University Medical School, Baltimore, Maryland. Glycotech. - [Read Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates]

Caspase 8 Assay Protocol - http://www.promega.com/tbs/tb332/tb332.pdf

Category: Pharmacology and Toxicology Protocols: Enzyme Assays Protocols

Protocol for caspase 8 assay. Includes: Detection of Caspase-8 Activity Using Purified Caspases; Detection of Caspase-8 Activity in Cell-Based Assays. - [Read Caspase 8 Assay Protocol]

Caspase 9 Assay Protocol - http://www.promega.com/tbs/tb333/tb333.pdf

Category: Pharmacology and Toxicology Protocols: Enzyme Assays Protocols

Protocol for caspase 9 assay. Includes: Detection of Caspase-9 Activity Using Purified Caspases; Detection of Caspase-9 Activity in Cell-Based Assays. - [Read Caspase 9 Assay Protocol]

Cell Adhesion Protocol - http://people.virginia.edu/~gwl6s/protocols/adhesion.html

Category: Cell Biology and Cell Culture

Protocol for cell adhesion. - [Read Cell Adhesion Protocol]

Cell Culture Guidelines - http://iacf.bsd.uchicago.edu/Monoclonal_Antibody_Facility/CellCultureGuidelines.htm

Category: Cell Biology and Cell Culture: Troubleshooting Cell Culture Problems

Immunology Applications Core Facility, Univ. Chicago - [Read Cell Culture Guidelines]

Articles (1-10 of 28)

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

Microinjection Pipettes Protocol

Microinjection Pipettes Preparation Protocol.

Acid Washing Microinjection Pipettes Protocol

Acid Washing of Glass microinjection pipettes protocol.

Paraffin Embedding Protocol

Paraffin Embedding Protocol for molecular profiling. This Paraffin Embedding Protocol describes the processing of the tissues into sections following ethanol fixation. Molecular profiling (MP) is a technique that is used to visualize the global patterns of RNA expression or protein expression in various cell types and disease processes.

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