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Antibody Addition and Detection for Staining Caenorhabditis elegans Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4523

Category: C. elegans Protocols: C. elegans Staining Protocols

Extreme care should be used to identify and verify positive reactions, however, because cross-reactions are common. Counterstaining is essential for examining worms by immunofluorescence and is used to identify the exact cell in which an antigen appears. Methods for counterstaining include labeling all cells with a fluorescent dye that is specific for nucleic acids (e.g., DAPI or propidium iodide) and using GFP driven by tissue-specific promoters. - [Read Antibody Addition and Detection for Staining Caenorhabditis elegans Protocol]

Arabidopsis transformation with pictures - http://www.bioinformatics.vg/Methods/arabitransformationf.htm

Category: Plant Biology Protocols: Arabidopsis Transformation Protocols

Arabidopsis transformation and selection on kanamycin agar, Preparation of plants for transformation, Agrobacterium, Dipping, Post-dip care, Agar plates, Tips. Adaptation of the short protocol described bySteve Clough and Andrew Bent, University of Illinois at Urbana-Champaign. - [Read Arabidopsis transformation with pictures]

Centrifuge Safety - http://www.chm.bris.ac.uk/safety/centrifg.htm

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Hazards of Centrifuge use in the laboratory and Operation. Rotor care, and Pre-run safety checks for rotors and centrifuges. - [Read Centrifuge Safety]

Centrifuge Safety - High Speed Hazards - http://www.ehs.wsu.edu/Factsheets/FAQCentrifuge.asp

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Centrifuge Safety - High Speed Hazards. Safe Centrifuge Use , Care and Maintenance, and maintaining a log book for centrifuges. EHS Washington state Univ. - [Read Centrifuge Safety - High Speed Hazards]

Essentials for Animal Research - A Primer for Research Personnel - a handbook - http://research.ucsb.edu/connect/acc/ess_idex.html

Category: Animal Research Techniques

Institutional Animal Care and Use Committee. Office of Research, UCSB. - [Read Essentials for Animal Research - A Primer for Research Personnel - a handbook]

Hints and Precautions for the Care, Feeding and Breeding of Neurospora - http://www.fgsc.net/methods/perkins.html

Category: Fungi Protocols: Neurospora Protocols

Hints and precautions for the care, feeding and breeding of Neurospora. Includes: Crossing; Tips on handling ascospoes General Laboratory Practices; Stockkeeping; Mutagenesis and enrichment; Tips for encouraging colonial growth; Solutions and media; Handling Heterokaryons. - [Read Hints and Precautions for the Care, Feeding and Breeding of Neurospora]

HPLC Column Care Protocols and Information - http://www.nestgrp.com/pdf/colcare.pdf

Category: Chromatography Protocols: Liquid Chromatography Protocols

Protocols and information on general HPLC column care. Includes: Silica based columns and Polymer based columns. Protocols included: pH stability, Mechanical stability, Mobile phases (Eluents), Proper storage of HPLC columns, Equilibration time, Regeneration of a column, Regeneration of RP packings, Regeneration of NP (Normal Phase) packings, Regeneration of Ion Exchange Packings - [Read HPLC Column Care Protocols and Information]

John Hopkins Animals Research Use and Care - http://www.jhu.edu/animalcare/training_procedures_animal_numbers.html

Category: Animal Research Techniques

Minimization of Animal Numbers Used to Obtain Valid Results. - [Read John Hopkins Animals Research Use and Care]

Light Microscopy - http://www.ruf.rice.edu/%7Ebioslabs/methods/microscopy/microscopy.html

Category: Molecular Imaging: Light Microscopy

Types of light microscopy, Bright Field Microscopy, Using a bright field microscope, mounting on slides, adjusting the microscope, Care of the microscope, When to use bright field microscopy. David R. Caprette. Rice University. - [Read Light Microscopy]

Media for Culture of Mammalian Cells Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662B6F09C1BB8FBC47FC2A7E61A8A3&objectid=66739B890A55AC13354418E67F28E693

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Mammalian Cell Culture Growth Media Protocols

The culture medium is an essential component of the in vitro environment and must be selected or designed with care. This protocol provides guidelines for design of serum-containing and serum-free media, selective and specialty media, and media for growth under special conditions such as soft-agar growth. - [Read Media for Culture of Mammalian Cells Protocol]

Preparation of an Ion-Exchange Column Protocol - http://cshprotocols.cshlp.org/cgi/content/extract/2006/1/pdb.prot4195

Category: Chromatography Protocols: Ion Exchange Chromatography Protocols

Protocol for the preparation of ion-exchange chromatography column. Ion-exchange chromatography (IEC) can be used as a crude step in a protein purification scheme, or, with proper preparation, as a high-resolution step. If high resolution is desired, considerable care should be taken during column preparation, choice of IEC media, and column packing. - [Read Preparation of an Ion-Exchange Column Protocol]

Preparation of Endothelial Cells Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=E538DDD4A302A0DF2CDDD9363072C1CA&objectid=6673B460F50B90057960CE47ABB708A4

Category: Cell Biology and Cell Culture: Endothelial Cell Protocols

Endothelial cells, which line blood vessels, can be prepared from a variety of tissues. They are frequently prepared from the umbilical vein, which is relatively easy to obtain. The procedure is clearly described and provides a large population of highly purified endothelial cells. There are also methods for obtaining endothelial cells from other tissues such as fat, skin, and mucosa. These methods require special care and generate smaller populations of cells. - [Read Preparation of Endothelial Cells Protocol]

Purification of Yeast Artificial Chromosome (YAC) DNA with Filtration Units Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4393

Category: Yeast Protocols: Yeast Genetics Protocols: YACs Protocols

Protocol describes a method for purification of YAC DNA using ultrafiltration. This is the fastest method available, but care needs to be taken not to damage the DNA. The purified DNA can then be used for microinjection. - [Read Purification of Yeast Artificial Chromosome (YAC) DNA with Filtration Units Protocol]

Articles (1-5 of 5)

Probe Preparation for 22 x 40 mm DNA Microarrays

Probe Preparation for 22 x 40 mm DNA Microarrays Protocol.

DNA Fragment Purification from Polyacrylamide Gels Protocol

A simple protocol for the purification of DNA fragments from Polyacrylamide Gels.

Quick DNA Agarose Gel Electrophoresis Protocol.

A quick and simple protocol for the electrophoresis of DNA fragments using agarose gels.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.

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