User Panel

My Panel

Bookmark Science Articles

cancer Protocols

Search results for: cancer

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

A Method for Micro-Scale Isolation and Purification of Gangliosides - http://www.glycotech.com/protocols/Proto1.html

Category: Molecular Biology Protocols: Carbohydrate Protocols

Solvent partition protocol allows the isolation of gangliosides from small samples and from samples where ganglioside concentrations are low, especially relative to the concentration of potentially contaminating proteins and other large molecular weight species. Stephan Ladisch Director, Center for Cancer and Transplant Biology, Children's National Medical Center, Washington, D.C. - [Read A Method for Micro-Scale Isolation and Purification of Gangliosides]

Aggregation: Production of Chimerae - http://transgenics.ufscc.ufl.edu/TCF05agg_instruct.html

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: ES Cell Aggregations - Production of Chimerae

Aggregation: Production of Chimerae. Detailed ES cell growth and preparation information for aggregations. Edward W. Scott Univ. Florida Shands Cancer Center - [Read Aggregation: Production of Chimerae]

Applications of SKY in Cancer Cytogenetics - http://www.utoronto.ca/cancyto/protocols_software/cytogenetics/SKY.html

Category: Cytogenetics Protocols: Spectral Karyotyping SKY Protocols

SKY has been applied to various tumor groups including hematological malignancies, sarcomas, carcinomas and brain tumors, with the intent of identifying specific chromosomal abnormalities that may provide insight to the genes involved in the disease process as well as identifying recurrent cytogenetic markers for clinical diagnosis and prognostic assessment. - [Read Applications of SKY in Cancer Cytogenetics]

Applications of SKY in Cancer Cytogenetics Review - http://www.utoronto.ca/cancyto/protocols_software/cytogenetics/SKY.html

Category: Genetics and Genomics: Cancer Genetics Protocols

SKY has also been applied for the mouse genome, enabling investigators to extrapolate information from mouse models of cancer to their human counterparts. This review will address the advances that SKY has facilitated in the field of cancer cytogenetics, as well as its variety of application in the cancer research laboratories. - [Read Applications of SKY in Cancer Cytogenetics Review]

Bisulfite Treatment of DNA for Methylation Analysis - http://www.mdanderson.org/departments/methylation/display.cfm?id=955D5740-37D4-444F-ACF0193F8EC3989B&method=displayFull

Category: Epigenetics and Methylation Protocols: Bisulfite Treatment Methylation Assay Protocol

Adapted from Frommer et.al. Good protocol for bisulfite treatment of DNA. Includes tips on Methylation PCR for CpG methylation analysis. University of Texas M. D. Anderson Cancer Center - [Read Bisulfite Treatment of DNA for Methylation Analysis]

Bisulfite-PCR for Restriction Analysis and/or Sequencing - http://www.mdanderson.org/departments/methylation/display.cfm?id=8E0AAAAC-FA42-486C-A93F60CD6E1C7E51&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: Epigenetics and Methylation Protocols

Bisulfite-PCR for Restriction Analysis and/or Sequencing. Protocol written by Jean-Pierre Issa. M.D Anderson Cancer Centre Texas. - [Read Bisulfite-PCR for Restriction Analysis and/or Sequencing]

Calcium Flux Using Indo-1 on the FACSVantage Protocol - http://www.cancer.umn.edu/images/research/cores/calcflux.jpg

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols

Protocol for calcium flux using Indo-1 on the FACSVantage. - [Read Calcium Flux Using Indo-1 on the FACSVantage Protocol]

Detection of Changes in Mitochondrial Membrane Potential on the FACSCalibur - http://www.cancer.umn.edu/images/research/cores/mitomemb.jpg

Category: Cell Organelle Protocols: Mitochondria Protocols

Protocol for the detection of changes in mitochondrial membrane potential on the FACSCalibur. - [Read Detection of Changes in Mitochondrial Membrane Potential on the FACSCalibur]

Determination of the CpG Island Methylator Phenotype (CIMP) in Colorectal Cancer using MethyLight - http://www.natureprotocols.com/2006/06/30/determination_of_the_cpg_islan.php

Category: Genetics and Genomics: Cancer Genetics Protocols

Detailed, step-by-step protocol of the MethyLight assay for detection of CIMP with high sensitivity and specificity in colorectal cancer using a five marker panel composed of CACNA1G, IGF2, NEUROG1, RUNX3 and SOCS1. - [Read Determination of the CpG Island Methylator Phenotype (CIMP) in Colorectal Cancer using MethyLight]

Determination of the CpG Island Methylator Phenotype (CIMP) in colorectal cancer using MethyLight - http://www.natureprotocols.com/2006/06/30/determination_of_the_cpg_islan.php

Category: Nucleic Acid Modification Protocols: DNA Methylation Protocols

Determination of the CpG Island Methylator Phenotype (CIMP) in colorectal cancer using MethyLight. Protocol describes a detailed, step-by-step protocol of the MethyLight assay for detection of CIMP with high sensitivity and specificity in colorectal cancer using a five marker panel composed of CACNA1G, IGF2, NEUROG1, RUNX3 and SOCS1. - [Read Determination of the CpG Island Methylator Phenotype (CIMP) in colorectal cancer using MethyLight]

Determination of the CpG Island Methylator Phenotype (CIMP) in colorectal cancer using MethyLight - http://www.natureprotocols.com/2006/06/30/determination_of_the_cpg_islan.php

Category: PCR Protocols: Real-Time PCR Protocols

Protocol provides a detailed, step-by-step protocol of the MethyLight assay for detection of CIMP with high sensitivity and specificity in colorectal cancer using a five marker panel composed of CACNA1G, IGF2, NEUROG1, RUNX3 and SOCS1. - [Read Determination of the CpG Island Methylator Phenotype (CIMP) in colorectal cancer using MethyLight]

DNA-Methyltransferase Assay - Anderson Centre - http://www.mdanderson.org/departments/methylation/display.cfm?id=9B5B6082-C53A-4A86-BEE3EE149A52251E&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: Epigenetics and Methylation Protocols

M.D. Anderson Cancer Centre Texas - [Read DNA-Methyltransferase Assay - Anderson Centre]

Four Color Immunophenotyping on the FACSCalibur Protocol - http://www.cancer.umn.edu/images/research/cores/4color.jpg

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Flow Cytometry Immunofluorescence Staining Protocols

Protocol for four color immunophenotyping on the FACSCalibur. - [Read Four Color Immunophenotyping on the FACSCalibur Protocol]

Gene-specific RT-PCR NCI - http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAnalysis/RNA/GeneSpecificRTPCR.html

Category: RNA Protocols: RT-PCR and cDNA synthesis

Gene-specific RT-PCR. Cancer Gene Anatomy Project NCI - [Read Gene-specific RT-PCR NCI]

Immunocytochemistry - http://cancer.ucsd.edu/howelllab/Immunocyto.html

Category: Immunology: Immunocytochemistry Protocols

Method for plating cells, fixing cells, antibody incubation and mounting of slides. PHEM buffer, Antifade recipes. Howell Lab. - [Read Immunocytochemistry]

M9 Plate Supplementation Table - http://www.fhcrc.org/science/labs/gottschling/bac/bsup.html

Category: Microbiology: Bacterial E.Coli Culture Media & Plates

M9 Plate Supplementation Table. URA, LEU, MET, HIS, TRP. Fred Hutchinson Cancer Research Center - [Read M9 Plate Supplementation Table]

Methylated CpG Island Amplification - http://www.mdanderson.org/departments/methylation/display.cfm?id=8A198378-986B-41FD-8778FA1399FBAB4D&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: Epigenetics and Methylation Protocols

Methylated CpG Island Amplification. Protocol written by Minoru Toyota. M.D Anderson Cancer Centre Texas. - [Read Methylated CpG Island Amplification]

Methylation Specific PCR Protocol - http://www.mdanderson.org/departments/methylation/display.cfm?id=0CB11381-59AE-4D90-AC0B343F2D646EDD&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: Epigenetics and Methylation Protocols

Methylation Specific PCR Protocol. M.D Anderson Cancer Centre Texas. Protocol written by James Herman* - [Read Methylation Specific PCR Protocol]

PCR Genotyping of Mutant Strains - http://www.fhcrc.org/science/labs/soriano/protocols/pcrgen.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

PCR Genotyping of Mutant Strains. Fred Hutchinson Cancer Research Center - [Read PCR Genotyping of Mutant Strains]

PCR Genotyping of Mutant Strains - http://www.fhcrc.org/science/labs/soriano/protocols/pcrgen.html

Category: PCR Protocols

PCR Genotyping of Mutant Strains. Fred Hutchinson Cancer Research Center - [Read PCR Genotyping of Mutant Strains]

PCR Protocol Buffer Creation - http://www.fhcrc.org/science/labs/soriano/protocols/pcr.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

PCR Protocol Buffer Creation. Based on Jane Gitschier's group.Fred Hutchinson Cancer Research Center. - [Read PCR Protocol Buffer Creation]

PCR Protocol Buffer Creation - http://www.fhcrc.org/science/labs/soriano/protocols/pcr.html

Category: PCR Protocols

PCR Protocol Buffer Creation. Based on Jane Gitschier's group.Fred Hutchinson Cancer Research Center. - [Read PCR Protocol Buffer Creation]

PCR Screens of ES Cell Clones - http://www.fhcrc.org/science/labs/soriano/protocols/pcres.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

Identification of targeted ES cell clones by PCR. Fred Hutchinson Cancer Research Center. - [Read PCR Screens of ES Cell Clones]

PCR Screens of ES Cell Clones - http://www.fhcrc.org/science/labs/soriano/protocols/pcres.html

Category: PCR Protocols

Identification of targeted ES cell clones by PCR. Fred Hutchinson Cancer Research Center. - [Read PCR Screens of ES Cell Clones]

Protein Kinase C Assay - http://cancer.ucsd.edu/newtonlab/Newton%20Web%20PKC%20ASSAY.html

Category: Signalling Signal Transduction Protocols: Protein Kinase Protocols

Protein Kinase C Assay, Newton lab. Using the peptide substrate Ac-FKKSFKL-NH2. Detailed Assay Protocol. - [Read Protein Kinase C Assay]

Items 1-25 out of 26 displayed.

First [1] 2 Next Last

Total records: 26

Protocols Tags

cytogenetics Protocols

detailed Protocols

detection Protocols

determination Protocols

dna Protocols

facscalibur Protocols

methylation Protocols

sensitivity Protocols

sky Protocols

socs1 Protocols

specific Protocols

specificity Protocols

Molecular Station Menu

Welcome to Molecular Station!

You have to register before you can post on our forums or use our advanced features. Register Now! Its Free and Fast!

Already registered? Login now below.

Already registered and Forgot your password? Click below to recover it.

Recover Lost Password

Home

Features

Protocols

DNA Forum

Science Forum

DNA Forum
Biology Forum

Member Login

User Panel

My Panel

My Panel

Bookmark Science Articles

cancer Protocols

Search Results Protocol Links Sort by: Hits | Alphabetical

Protocols Tags

Molecular Station Menu

Welcome to Molecular Station!

Protocols

Science Forum

Science News