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Phosphate (Sodium) buffer Chart - http://wheat.pw.usda.gov/~lazo/methods/lazo/phosphat.html

Phosphate (Sodium) buffer Chart. Easy to setup buffers using this chart. Gerard R. Lazo - [Read Phosphate (Sodium) buffer Chart]

Protocol: Luciferase assay protocol - http://medicine.ucsd.edu/hypertension/list_of_protocols/5_luciferase_assay.htm

Category: Luciferase and Dual Luciferase Assays

Protocol: Luciferase assay protocol. Simple Recipies for Luciferase assay buffers I and II. - [Read Protocol: Luciferase assay protocol]

ELISPOT Protocol Detailed PDF - http://www.biolegend.com/images/Protocols/BioLegend%20ELISPOT%20protocol.pdf

Category: ELISA Protocols: ELISpot Protocols

Coat the Plate, Block the Plate, Add Detection Antibody, Solutions and buffers for ELISpot. Biolegend. - [Read ELISPOT Protocol Detailed PDF]

Restriction Digestion Methods - http://hg.wustl.edu/hdk_lab_manual/rdm/rdmcontents.html

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

Restriction Digestion Methods. Donis-Kellor lab manual. Restriction Enzyme Digests, Restriction Enzyme Buffers, Restriction Digestion of Plasmid, Cosmid, and Phage DNAs. - [Read Restriction Digestion Methods]

Immunoprecipitation Protocol and Information - http://www.ebioscience.com/ebioscience/appls/IP.htm

Category: Protein Protocols: Immunoprecipitation Protocols

Immunoprecipitation Protocol and Information. IP. Cell Lysate Preparation, preclearing, Immunoprecipitation Buffers. Ebioscience. - [Read Immunoprecipitation Protocol and Information]

General Immunofluorescence Protocol - http://www.bcm.edu/rosenlab/protocols/generalIF.pdf

Category: Molecular Imaging: Immunofluorescence Microscopy

General Immunofluorescence Protocol. Rosen Lab. Deparaffinize and rehydrate sections, Antigen retrieval methods, Proteinase K Antigen Retrieval, Urea Antigen Retrieval, Blocking buffers, - [Read General Immunofluorescence Protocol]

Common Buffers - http://wheat.pw.usda.gov/~lazo/methods/lazo/buffer.html

Category: Buffers Media, and Solutions

Recipes for commonly used buffer solutions. (Gerard R. Lazo) - [Read Common Buffers]

Preparing a Buffer Solution - http://www.csudh.edu/oliver/chemdata/buffers.htm

Category: Buffers Media, and Solutions: Buffer Theory

Preparing a Buffer Solution. Oliver Seeley. - [Read Preparing a Buffer Solution]

Buffers for pH Control. Calculator tool. - http://www.bioinformatics.org/JaMBW//5/4/index.html

Category: Buffers Media, and Solutions

Buffers for pH Control. Calculator tool. Rob Beynon, 1996. - [Read Buffers for pH Control. Calculator tool.]

Buffer Solutions and How they work... - http://www.chemguide.co.uk/physical/acidbaseeqia/buffers.html

Category: Buffers Media, and Solutions: Buffer Theory

This page describes simple acidic and alkaline buffer solutions and explains how they work. - [Read Buffer Solutions and How they work...]

Detailed NF-kB EMSA Protocol - http://www.celldeath.de/apometh/emsa.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols: NF-kB EMSA Protocols

Detailed NF-kB EMSA Protocol. Includes Nuclear extract preparation, Labeling of the oligonucleotide probe: T4 Polynucleotide Kinase Reaction, Supershift assay to identify NF-kB, and recipes for EMSA buffers. Celldeath.de - [Read Detailed NF-kB EMSA Protocol]

ELECTRON MICROSCOPY (EM) protocols and methods - http://www.bristol.ac.uk/vetpath/cpl/emtechs.htm

Category: Molecular Imaging: Electron Microscopy Protocols

E.M. processing schedule - epoxy resin E.M. processing schedule - acrylic resin Primary fixative and buffers Veterinary Pathology. ELECTRON MICROSCOPY (EM) protocols and methods - [Read ELECTRON MICROSCOPY (EM) protocols and methods]

Immunoprecipitation Protocol - http://www.ebioscience.com/ebioscience/appls/IP.htm

Category: Immunology: Immunoprecipitation Protocols

Protocol for immunoprecipitation. Includes: Cell Lysate Preparation; Cell Lysate Preclearing; Immunoprecipitation; Immunoprecipitation Buffers. - [Read Immunoprecipitation Protocol]

Yeast Nuclear Extract (Small Scale) - http://www.fhcrc.org/science/labs/hahn/methods/biochem_meth/polii_nuc_ext.html

Category: Protein Protocols: Nuclear Extraction Protocols

Nuclear Extract preparation protocols, Buffers and solutions for nuclear extracts. Hahn Lab - [Read Yeast Nuclear Extract (Small Scale)]

Protocol for Primary Cultures of HUVECs - http://www.huvec.com/documents/Protocol-HUVECs.pdf

Category: Cell Biology and Cell Culture: Endothelial Cell Protocols

Protocol for primary cultures of HUVECs. Includes: Buffers and reagents (Fetal calf serum, Phosphate Buffer Saline (PBS), Collagenase, Buffer for conservation and transport of umbilical cords, Culture medium); Cell culture. - [Read Protocol for Primary Cultures of HUVECs]

Cytokine ELISPOT Protocol - http://www.ebioscience.com/ebioscience/appls/ELISPOT.htm#protocol

Category: ELISA Protocols: ELISpot Protocols

Protocol for cytokine ELISPOT. Includes: Description; Stability; Contents of the ELISPOT Ready-SET-Go! Reagent Set; ELISPOT Method; Cytokine ELISPOT Buffers. - [Read Cytokine ELISPOT Protocol]

Biological Buffers a reference - http://www.sigmaaldrich.com/Brands/Fluka___Riedel_Home/Bioscience/BioChemika_Ultra/Biological_Buffers.html

Category: Buffers Media, and Solutions: Buffer Theory

Biological Buffers a reference. Sigma - [Read Biological Buffers a reference]

Western Blot Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/W1.html

Category: Western Blot Protocols: General Western Blot Methods Protocols

This protocol was developed for the BIORAD protein gel and transfer apparatus. The buffers can be used with any electrophoresis/transfer system. - [Read Western Blot Protocol]

Lab Faqs: Buffers Roche - http://www.roche-applied-science.com/labfaqs/p4_1.htm

Category: Buffers Media, and Solutions

Lab Faqs: Buffer recipies. Whether you are making TAE or TBE this has got it all...Roche - [Read Lab Faqs: Buffers Roche]

DNA Affinity Chromatography Using Gravity Flow - Subscription Required - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4206

Category: Chromatography Protocols: DNA RNA Affinity Chromatography

DNA Affinity Chromatography, DNA affinity chromatography can be a low-tech method using gravity flow at 4Â°C, a disposable chromatography column, and DNA affinity resin prepared in the laboratory (see Preparation of a DNA Affinity Column). Include 10-20% glycerol and 0.025-0.1% NP-40 in the column buffers to suppress losses due to nonspecific adsorption of protein to surfaces. Load the protein in a buffer that is compatible with binding of the protein to its target site. Keith Brocklehurst et al - [Read DNA Affinity Chromatography Using Gravity Flow - Subscription Required]

Separating Proteins Using Ion-Exchange Chromatography Protocol - http://cshprotocols.cshlp.org/cgi/content/extract/2006/1/pdb.prot4196

Category: Chromatography Protocols: Ion Exchange Chromatography Protocols

Protocol for separating proteins using ion-exchange chromatography. Protocol details the practical considerations of an ion-exchange chromatography (IEC) experiment. The choice of what type of ion exchanger to use, as well as the composition of the buffers used in this experiment, should be determined prior to beginning this protocol. - [Read Separating Proteins Using Ion-Exchange Chromatography Protocol]

PCR Buffers Protocol - http://info.med.yale.edu/genetics/ward/tavi/p06.html

Category: PCR Protocols: Standard PCR Protocol

Protocol for PCR buffers. - [Read PCR Buffers Protocol]

PCR Buffers Protocol - http://info.med.yale.edu/genetics/ward/tavi/p06.html

Category: PCR Protocols: PCR Buffer Protocols

Protocol for PCR buffers. - [Read PCR Buffers Protocol]

Cytokine ELISPOT (Enzyme-linked ImmunoSPOT) Protocols - http://www.ebioscience.com/ebioscience/appls/ELISPOT.htm

Category: Signalling Signal Transduction Protocols: Cytokine Protocols

Cytokine Enzyme-linked immunosorbent spot (ELISPOT) assays. Cytokine ELISPOT Buffers and procedure protocol. - [Read Cytokine ELISPOT (Enzyme-linked ImmunoSPOT) Protocols]

Lambda Phage DNA Quickprep - http://www.unizh.ch/botinst/Cyto_Website/schneitzLab/Methods/DNAIsolation/phageQuickprep.html

Category: Molecular Biology Protocols: Phage Protocols and Methods

Lambda Phage DNA Quickprep with recipes for PSB, STE, l-dil and MgCa buffers and media. Kay Schneitz - [Read Lambda Phage DNA Quickprep]

Articles (1-10 of 16)

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

Acid Washing Microinjection Pipettes Protocol

Acid Washing of Glass microinjection pipettes protocol.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

Absorption Spectroscopy and Quantification of Filamentous Phage Protocol

Unlike spherical phage, such as T4 and λ, which have roughly equal weight ratios of protein to DNA, filamentous phage have about six times more protein than DNA; the protein therefore contributes substantially to the absorption spectrum.

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