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Activation of p70s6k and MAP Kinases: IP and Kinase Activity - http://www.whitelabs.org/Lab%20Protocols/kinase%20and%20phosphatase%20assays/ACTIVATION%20OF%20p70s6k%20and%20MAP%20KINASES.htm

Category: Signalling Signal Transduction Protocols: MAPK Protocols

Protocol describes the activation of MAP Kinase activity. Protocol contains information on: Buffers: ST, STE, Lysis Buffer Buffer A, Buffer B and 10x Kinase Buffer. - [Read Activation of p70s6k and MAP Kinases: IP and Kinase Activity]

Biological Buffers a reference - http://www.sigmaaldrich.com/Brands/Fluka___Riedel_Home/Bioscience/BioChemika_Ultra/Biological_Buffers.html

Category: Buffers Media, and Solutions: Buffer Theory

Biological Buffers a reference. Sigma - [Read Biological Buffers a reference]

Buffer Solutions and How they work... - http://www.chemguide.co.uk/physical/acidbaseeqia/buffers.html

Category: Buffers Media, and Solutions: Buffer Theory

This page describes simple acidic and alkaline buffer solutions and explains how they work. - [Read Buffer Solutions and How they work...]

Buffers for pH Control. Calculator tool. - http://www.bioinformatics.org/JaMBW//5/4/index.html

Category: Buffers Media, and Solutions

Buffers for pH Control. Calculator tool. Rob Beynon, 1996. - [Read Buffers for pH Control. Calculator tool.]

Common Buffers - http://wheat.pw.usda.gov/~lazo/methods/lazo/buffer.html

Category: Buffers Media, and Solutions

Recipes for commonly used buffer solutions. (Gerard R. Lazo) - [Read Common Buffers]

Cytokine ELISPOT (Enzyme-linked ImmunoSPOT) Protocols - http://www.ebioscience.com/ebioscience/appls/ELISPOT.htm

Category: Signalling Signal Transduction Protocols: Cytokine Protocols

Cytokine Enzyme-linked immunosorbent spot (ELISPOT) assays. Cytokine ELISPOT Buffers and procedure protocol. - [Read Cytokine ELISPOT (Enzyme-linked ImmunoSPOT) Protocols]

Cytokine ELISPOT Protocol - http://www.ebioscience.com/ebioscience/appls/ELISPOT.htm#protocol

Category: ELISA Protocols: ELISpot Protocols

Protocol for cytokine ELISPOT. Includes: Description; Stability; Contents of the ELISPOT Ready-SET-Go! Reagent Set; ELISPOT Method; Cytokine ELISPOT Buffers. - [Read Cytokine ELISPOT Protocol]

Detailed NF-kB EMSA Protocol - http://www.celldeath.de/apometh/emsa.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols: NF-kB EMSA Protocols

Detailed NF-kB EMSA Protocol. Includes Nuclear extract preparation, Labeling of the oligonucleotide probe: T4 Polynucleotide Kinase Reaction, Supershift assay to identify NF-kB, and recipes for EMSA buffers. Celldeath.de - [Read Detailed NF-kB EMSA Protocol]

DNA Affinity Chromatography Using Gravity Flow - Subscription Required - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4206

Category: Chromatography Protocols: DNA RNA Affinity Chromatography

DNA Affinity Chromatography, DNA affinity chromatography can be a low-tech method using gravity flow at 4Â°C, a disposable chromatography column, and DNA affinity resin prepared in the laboratory (see Preparation of a DNA Affinity Column). Include 10-20% glycerol and 0.025-0.1% NP-40 in the column buffers to suppress losses due to nonspecific adsorption of protein to surfaces. Load the protein in a buffer that is compatible with binding of the protein to its target site. Keith Brocklehurst et al - [Read DNA Affinity Chromatography Using Gravity Flow - Subscription Required]

ELECTRON MICROSCOPY (EM) protocols and methods - http://www.bristol.ac.uk/vetpath/cpl/emtechs.htm

Category: Molecular Imaging: Electron Microscopy Protocols

E.M. processing schedule - epoxy resin E.M. processing schedule - acrylic resin Primary fixative and buffers Veterinary Pathology. ELECTRON MICROSCOPY (EM) protocols and methods - [Read ELECTRON MICROSCOPY (EM) protocols and methods]

ELISPOT Protocol Detailed PDF - http://www.biolegend.com/images/Protocols/BioLegend%20ELISPOT%20protocol.pdf

Category: ELISA Protocols: ELISpot Protocols

Coat the Plate, Block the Plate, Add Detection Antibody, Solutions and buffers for ELISpot. Biolegend. - [Read ELISPOT Protocol Detailed PDF]

General Immunofluorescence Protocol - http://www.bcm.edu/rosenlab/protocols/generalIF.pdf

Category: Molecular Imaging: Immunofluorescence Microscopy

General Immunofluorescence Protocol. Rosen Lab. Deparaffinize and rehydrate sections, Antigen retrieval methods, Proteinase K Antigen Retrieval, Urea Antigen Retrieval, Blocking buffers, - [Read General Immunofluorescence Protocol]

Hybridization of Oligonucleotide Probes in Aqueous Solutions Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3883

Category: Nucleic Acid Modification Protocols: Nucleic Acid Labeling Protocols

Hybridization is carried out in conventional aqueous solvents at a temperature well below the predicted melting temperature. Nonspecific hybrids are then removed by washing at high stringency in buffers containing quaternary salts. Tetramethylammonium chloride (TMACl) is used with probes that are 14-50 nucleotides in length, whereas tetraethylammonium chloride (TEACl) is used with longer oligonucleotides. - [Read Hybridization of Oligonucleotide Probes in Aqueous Solutions Protocol]

Immunoprecipitation Protocol - http://www.ebioscience.com/ebioscience/appls/IP.htm

Category: Immunology: Immunoprecipitation Protocols

Protocol for immunoprecipitation. Includes: Cell Lysate Preparation; Cell Lysate Preclearing; Immunoprecipitation; Immunoprecipitation Buffers. - [Read Immunoprecipitation Protocol]

Immunoprecipitation Protocol and Information - http://www.ebioscience.com/ebioscience/appls/IP.htm

Category: Protein Protocols: Immunoprecipitation Protocols

Immunoprecipitation Protocol and Information. IP. Cell Lysate Preparation, preclearing, Immunoprecipitation Buffers. Ebioscience. - [Read Immunoprecipitation Protocol and Information]

Isolation of Arabidopsis Nuclei and Measurement of Gene Transcription Rates Protocol - http://www.natureprotocols.com/2007/01/25/isolation_of_arabidopsis_nucle.php

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

Protocol for the isolation of Arabidopsis nuclei and measurement of gene transcription rates using nuclear run-on assays. Plant materials are ground in hexylene glycol-based buffers and highly enriched nuclear fractions are obtained using Percoll density gradients. Standard and small-scale protocols are presented, along with a tested method for nuclear run-on assays. The entire process may be completed within 3 days. - [Read Isolation of Arabidopsis Nuclei and Measurement of Gene Transcription Rates Protocol]

Lab Faqs: Buffers Roche - http://www.roche-applied-science.com/labfaqs/p4_1.htm

Category: Buffers Media, and Solutions

Lab Faqs: Buffer recipies. Whether you are making TAE or TBE this has got it all...Roche - [Read Lab Faqs: Buffers Roche]

Lambda Phage DNA Quickprep - http://www.unizh.ch/botinst/Cyto_Website/schneitzLab/Methods/DNAIsolation/phageQuickprep.html

Category: Molecular Biology Protocols: Phage Protocols and Methods

Lambda Phage DNA Quickprep with recipes for PSB, STE, l-dil and MgCa buffers and media. Kay Schneitz - [Read Lambda Phage DNA Quickprep]

Measurement of Intracellular Ions by Flow Cytometry Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E661C4C9AC9935B31A1C2087E2276A3&objectid=6674EF32EE59A37C48B4A267D733CAE2

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols

Describes flow cytometric protocols using the dyes Indo-1 AM, Fluo-3, and Fura Red AM to measure intracellular calcium concentration. Support protocols detail the use of calcium buffers to calibrate a flow cytometric calcium assay, and methods to facilitate dye loading; an alternate protocol describes the use of a spectrofluorimeter to measure intracellular calcium for those investigators without access to a flow cytometer. - [Read Measurement of Intracellular Ions by Flow Cytometry Protocol]

Northern Hybridization Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3723

Category: Nucleic Acid Detection Protocols: Nucleic Acid Blotting Protocols

Protocol for northern hybridization. Protocol describes how to carry out northern hybridization at high stringency in phosphate-SDS-buffers. Although a wide variety of formats are available, hybridization is usually performed in heat-sealable bags, roller bottles, or plastic boxes, as described here. - [Read Northern Hybridization Protocol]

PCR Buffers Protocol - http://info.med.yale.edu/genetics/ward/tavi/p06.html

Category: PCR Protocols: PCR Buffer Protocols

Protocol for PCR buffers. - [Read PCR Buffers Protocol]

PCR Buffers Protocol - http://info.med.yale.edu/genetics/ward/tavi/p06.html

Category: PCR Protocols: Standard PCR Protocol

Protocol for PCR buffers. - [Read PCR Buffers Protocol]

Phosphate (Sodium) buffer Chart - http://wheat.pw.usda.gov/~lazo/methods/lazo/phosphat.html

Category: Buffers Media, and Solutions

Phosphate (Sodium) buffer Chart. Easy to setup buffers using this chart. Gerard R. Lazo - [Read Phosphate (Sodium) buffer Chart]

Preparation of Electrolyte Gradient Gels Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3795

Category: Nucleic Acid Electrophoresis Protocols

Protocol for the preparation of electrolyte gradient gels. Electrolyte gradients are formed when buffers of different concentrations are used in the upper (low electrolyte concentration) and lower (high electrolyte concentration) chambers of the electrophoresis device. - [Read Preparation of Electrolyte Gradient Gels Protocol]

Preparing a Buffer Solution - http://www.csudh.edu/oliver/chemdata/buffers.htm

Category: Buffers Media, and Solutions: Buffer Theory

Preparing a Buffer Solution. Oliver Seeley. - [Read Preparing a Buffer Solution]

Articles (11-16 of 16)

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol. This protocol contains the steps for 3' end rapid amplification of mRNA by PCR. The first-strand cDNA is synthesized from total or poly(A+) RNA by priming from the poly-A tail of the mRNA using a oligo (dT) adaptor primer. The cDNA is then amplified via PCR using a gene-specific primer and an adaptor primer.

Histone H1 Kinase Activity Assay

Histone H1 Kinase Activity Assay Protocol. This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.