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A Sensitive ELISA-Based Assay for Glycosyltransferases - http://www.glycotech.com/protocols/Proto4.html

Category: Molecular Biology Protocols: Carbohydrate Protocols

The method allows the detection and quantification of glycosyltransferase activity using an ELISA-based procedure and carbohydrate-specific monoclonal antibodies. Avoids the use of radiolabeled substrates. Bruce A. Macher~Professor of Chemistry and Biochemistry, San Francisco State University, San Francisco, CA - [Read A Sensitive ELISA-Based Assay for Glycosyltransferases]

Concentration of Oligo DNA by Ethanol Precipitation Protocol - http://mycoplasmas.vm.iastate.edu/lab_site/methods/DNA/precip.html

Category: Oligonucleotide Protocols: Oligonucleotide Purification Protocols

Concentration of DNA by Ethanol Precipitation Protocol. Adapted from Bruce A. Roe, Department of Chemistry and Biochemistry, The University of Oklahoma, Norman, Oklahoma. Usually 2.5 - 3 volumes of ethanol and/or acetate solution is added to the DNA in a microcentrifuge tube. This is then put into an ice-water bath for at least 10 minutes. The precipitation is performed by incubation at -20C overnight. - [Read Concentration of Oligo DNA by Ethanol Precipitation Protocol]

Confocal Laser Scanning Microscopy - http://www.celanphy.science.ru.nl/Bruce%20web/scanning%20microscopy.htm

Category: Microscopy Protocols: Confocal Microscopy Protocols

There are two major forms of laser scanning microscopy: confocal laser scanning microscopy (CLSM) and multiphoton laser scanning microscopy (MPLSM). Information on: X-t scans and X-Y scans; Confocal Laser Scanning Microscopy; Multiphoton Laser Scanning Microscopy; MPLSM requires no pin hole; Advantages of MPLSM over CLSM. - [Read Confocal Laser Scanning Microscopy]

Fragment purification on Sephacryl S-500 spin columns - http://www.genome.ou.edu/protocol_book/protocol_partI.html#I.H

Category: Chromatography Protocols: Column Chromatography Protocols

Fragment purification on Sephacryl S-500 spin columns , Bruce A. Roe. - [Read Fragment purification on Sephacryl S-500 spin columns]

Methods for DNA isolation - Protocol Book - http://www.genome.ou.edu/protocol_book/protocol_partIII.html#III

Category: DNA Protocols: DNA Extraction Protocols

Large scale double-stranded DNA isolation, Midiprep double-stranded DNA isolation. Bruce A. Roe, Department of Chemistry and Biochemistry, The University of Oklahoma, Norman. - [Read Methods for DNA isolation - Protocol Book]