bradford Protocols

Category: Reporter Gene Assays: B-Galactosidase Assay Protocols

There are two basic methods for the in vitro assay of B-galactosidase activity from yeast. They differ mainly in the method of preparing the material for assay. Both methods are described with accompanying protocols. Method I: Assay of Crude Extracts includes: Yeast Cell Growth; Yeast Cell Harvest; B-gal assays; Bradford Assays. Method II: Permeabilized cell assay. - [Read Assay of β-Galactosidase in Yeast Protocol]

Category: Yeast Protocols: Yeast Genetics Protocols: B-Galactosidase Assay Protocols

Protocol for assay of β-Galactosidase in Yeast. Includes: Assay of Crude Extracts; Yeast Cell Growth; Yeast Cell Harvest; β-gal assays; Bradford Assays; Permeabilized cell assay. - [Read Assay of β-Galactosidase in Yeast Protocol]

Category: Protein Protocols: Protein Quantitation Concentration Protocols: Bradford Protein Assay Protocols

Biorad Protein Assay Bradford. Contributor: Suprya Jayadev. - [Read Biorad Protein Assay Bradford]

Category: Protein Protocols: Protein Quantitation Concentration Protocols: Bradford Protein Assay Protocols

The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration.  It involves the binding of Coomassie Brilliant blue to protein. There is no interference from cations nor from carbohydrates such as sucrose. 
Detergents such as sodium dodecyl sulfate and triton x-100 can interfere with the assay, as well as strongly alkaline solutions.

Includes a general overview of the procedure and preparation of the standards in the protocol. - [Read Bradford Assay Method]

Category: Protein Protocols: Protein Quantitation Concentration Protocols: Bradford Protein Assay Protocols

A detailed and well thought out Bradford protein assay page using a spectrophotometer. Includes information such as the Bradford assay is very fast and uses about the same amount of protein as the Lowry assay, comments, procedural steps, equipment used and more. - [Read Bradford Protein Assay]

Category: Protein Protocols: Protein Quantitation Concentration Protocols: Bradford Protein Assay Protocols

Bradford Protein Assay Spectrophotometry.  Includes spectrophotometry information and the Bradford protein assay: A spectrophotometer or colorimeter makes use of the transmission of light through a solution to determine the concentration of a solute within the solution.  A spectrophtometer differs from a colorimeter in the manner in which light is separated into its component wavelengths.  A spectrophotometer uses a prism to separate light and a colorimeter uses filters. - [Read Bradford Protein Assay Spectrophotometry]

Category: Protein Protocols: Protein Quantitation Concentration Protocols: Bradford Protein Assay Protocols

Includes Abbreviations, Background, and Procedure steps using BSA. The Bradford protein assay (1) is one of several simple methods commonly used to determine the total protein concentration of a sample.  The method is based on the proportional binding of the dye Coomassie to proteins. The assay is colorimetric; as the protein concentration increases, the color of the test sample becomes darker.  Coomassie absorbs at 595 nm.  - [Read Bradford Protein Concentration Assay]

Category: Protein Protocols: Protein Quantitation Concentration Protocols: Bradford Protein Assay Protocols

Detailed methodology on the Bradford assay. - [Read Bradford Protein Quantification Assay Protocol]

Category: Reporter Gene Assays

Protocol for GUS reporter gene assay. Includes: Protein isolation; Alternative method for small (<1g) quantities of tissue; GUS assays; Bradford protein concentration determination assays - [Read GUS Reporter Gene Assay Protocol]

Category: General Research Protocols and Methods: Spectrophotometry Protocols

Bradford Protein Assay, Lowry Protein Assay, Biuret Assay. Protein concentration measurement. Dr. Heidcamp, Department of Biology, Gustavus Adolphus College. - [Read Spectrophotometry]

Category: Protein Protocols: Protein Quantitation Concentration Protocols: Bradford Protein Assay Protocols

Use of the Bradford Protein Assay in a Microtiter Plate Format Protocol. Includes a short introduction to the Bradford assy, materials and procedure. - [Read Use of the Bradford Protein Assay in a Microtiter Plate Format]