bouin’s Protocols

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Fixation of Mouse Embryos and Tissues Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4702

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol provides methods for fixation of mouse embryos and tissues with paraformaldehyde, Bouin’s fixative, or methanol/DMSO solution. - [Read Fixation of Mouse Embryos and Tissues Protocol]

Fixation. - http://www.dakousa.com/ishbfixation.pdf

Category: Histology Protocols: Histological Fixation

FORMALDEHYDE-BASED FIXATIVES, PARAFFIN-EMBEDDED SECTIONS, BOUINâ€™S SOLUTION, MERCURIC CHLORIDE-BASED FIXATIVES. FARMILO et al. - [Read Fixation.]

Fixing Caenorhabditis elegans in Bouin’s Fixative Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4521

Category: C. elegans Protocols: C. elegans Fixing Protocols

Bouin’s fixative is a particularly good choice for worms because it penetrates dense tissues well and is extremely good for fixing antigens. Like all strong fixatives, however, it is unsuitable for some antibody-antigen pairs. In such cases, the length of time in the Bouin’s fixative can be shortened, or paraformaldehyde fixation can be used instead. - [Read Fixing Caenorhabditis elegans in Bouin’s Fixative Protocol]

Fixing Caenorhabditis elegans in Paraformaldehyde Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4522

Category: C. elegans Protocols: C. elegans Fixing Protocols

Common method for fixing worms is to use paraformaldehyde. This method provides a gentler fixation than the Bouin’s method, but often requires the use of collagenase. This method is particularly good for examining adult worms. - [Read Fixing Caenorhabditis elegans in Paraformaldehyde Protocol]