User Panel

My Panel

Bookmark Science Articles

blot Protocols

Search results for: blot

Protocols » Search Results

Featured Search Results Links

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

Troubleshooting Western Blot - http://www.molecularstation.com/protein/troubleshooting-western-blots/

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Covers Many Western Blot Problems and Includes Many Solutions. Fuzzy Bands, Low or Weak Signal, High Background, Spots on Film, Too Many Bands. A MolecularStation Guide. - [Read Troubleshooting Western Blot]

Western Blot Info - http://www.westernblotinfo.com

Category: Protein Protocols: Western Blot Protocol

Western Blot Information, forum, and protocols. - [Read Western Blot Info]

Search Results Protocol Links Sort by: Hits | Alphabetical

Stripping Immunoblots for Reprobing or Storage Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4273

Category: Western Blot Protocols: Immunoblot Stripping Protocols

Protocol describes the practice of stripping immunoblots and reprobing to detect multiple proteins on the same blot is a good method to examine two antigens under identical conditions. - [Read Stripping Immunoblots for Reprobing or Storage Protocol]

Telomere Length Determination Protocol - http://www-rcf.usc.edu/~forsburg/telomere.html

Category: Cell Biology and Cell Culture

Telomere length can vary from strain to strain and is sensitive to a variety of mutants affecting DNA and chromosome function. Includes: Gel Protocol; Southern blot using oligo probe. - [Read Telomere Length Determination Protocol]

Too many bands on a Western blot FAQs SYSY Synaptic Sys. - http://www.sysy.com/faqs/western.html

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Too many bands on a Western blot FAQs SYSY Synaptic Sys. - [Read Too many bands on a Western blot FAQs SYSY Synaptic Sys.]

Troubleshooting Guide for Chemiluminescent Western Blotting Pierce - http://www.piercenet.com/Objects/View.cfm?type=Page&ID=0C7FDAA1-EB8C-42AD-90DE-5EA92505449F#trbleshoot

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Troubleshooting Guide for Chemiluminescent Western Blotting Pierce. High Background, Reverse image on film, Blot glows in the darkroom, Signal duration is less than 8 hours, Weak or no signal, Spots within the protein bands, non-specific bands. - [Read Troubleshooting Guide for Chemiluminescent Western Blotting Pierce]

Troubleshooting Guide: Western Blot - http://www.rndsystems.com/tsg_detail_objectname_western_blot.aspx

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Problem: Poor Transfer, Problem: Poor Transfer. R&D Systems. - [Read Troubleshooting Guide: Western Blot]

Troubleshooting the immunoprecipitation procedure IP and IB - Roche - http://www.roche-applied-science.com/PROD_INF/MANUALS/epitope/p66.pdf

Category: Protein Protocols: Immunoprecipitation Protocols: Immunoprecipitation Troubleshooting

Troubleshooting the immunoprecipitation procedure Roche. Tagged proteins not visible or only weakly visible on Western blot. High background or many nonspecific bands on gel or blot membrane. - [Read Troubleshooting the immunoprecipitation procedure IP and IB - Roche]

Troubleshooting the Western blot procedure Roche PDF - http://www.roche-applied-science.com/PROD_INF/MANUALS/epitope/p34-35.pdf

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Troubleshooting the Western blot procedure Roche. PDF - [Read Troubleshooting the Western blot procedure Roche PDF]

Virion RNA Dot Blot Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p617

Category: Viral Protocols

Protocol describes the production of a dot blot membrane spotted with RNA isolated from virus particles. - [Read Virion RNA Dot Blot Protocol]

Western Blot - http://www.biochem.emory.edu/labs/acorbe2/protocols9.html

Category: Protein Protocols: Western Blot Protocol

Simple Procedure for Western Blotting. Corbett Lab. - [Read Western Blot]

Western Blot Analysis of Endogenous Gene Expression Protocol - http://www.flemingtonlab.com/Protocols/WestBlotAnalyofEndogProts.pdf

Category: Western Blot Protocols

Procedure describes how it denatures most of the modification and degradation proteins immediately giving the most accurate read out of the true levels of protein at the time of harvest. However, in cases where detection is a problem, a limited purification (e.g. isolation of nuclear extract for the detection of transcription factors) might be required to allow analysis. - [Read Western Blot Analysis of Endogenous Gene Expression Protocol]

Western Blot Analysis of Sub-Cellular Fractionated Samples Using the Odyssey Infrared Imaging System - http://www.natureprotocols.com/2006/09/15/western_blot_analysis_of_subce.php

Category: Cell Organelle Protocols: Subcellular Fractionation Protocols

This protocol describes nuclear and cytoplasmic fractionation of tissue culture cells and a method for Western blot detection of proteins using the Odyssey Infrared Imaging System. This protocol was used to detect expression of the "small" Tap protein in 293T, HeLa and COS cells. The Odyssey system has several advantages over the more widely used chemiluminescent detection methods. - [Read Western Blot Analysis of Sub-Cellular Fractionated Samples Using the Odyssey Infrared Imaging System]

Western Blot Analysis—Phosphoprotein-Specific Antibody Mixture Protocol - http://www.afcs.org/reports/v1/BC0001/Protocols/WBPhosphoSpecific.pdf

Category: Immunology: Immunoassays

3-step process of Western immunoblotting for detection of particular sites of phosphorylation on specific proteins recognized by modification-sensitive antibodies. - [Read Western Blot Analysis—Phosphoprotein-Specific Antibody Mixture Protocol]

Western Blot Detailed (PDF) - http://www.its.caltech.edu/%7Ebjorker/Western_blot.pdf

Category: Protein Protocols: Western Blot Protocol

Protocol includes cell lysate preparation, SDS-PAGE gels, transfer, and blotting. (Bjorkman Group). - [Read Western Blot Detailed (PDF)]

Western Blot Protocol - http://www.sgul.ac.uk/depts/immunology/~dash/group/western.html

Category: Protein Protocols: Western Blot Protocol

Nice Tips on reducing sample viscosity, buffer preparation, and western blot protocol. Phil Dash. - [Read Western Blot Protocol]

Western Blot Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/W1.html

Category: Western Blot Protocols: General Western Blot Methods Protocols

This protocol was developed for the BIORAD protein gel and transfer apparatus. The buffers can be used with any electrophoresis/transfer system. - [Read Western Blot Protocol]

Western Blot Protocol and Theory - http://www.rndsystems.com/western_blot_detail_objectname_WesternBlotProtocol.aspx

Category: Protein Protocols: Western Blot Protocol

Theory and Protocol for Western Blotting / immunoblotting. R&D Systems. - [Read Western Blot Protocol and Theory]

Western Blot Troubleshooting - http://www.westernblotinfo.com/western-blot-troubleshooting/

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Streaking of Western Blots, Weak Bands or Weak Staining of Western Blots, Poor Transfer of Membranes, Dirty Blot, Multiple Bands on Western Blot Membrane and Film. Western Blot Info. - [Read Western Blot Troubleshooting]

Western Blotting with Biotinylated Antibodies - http://www.bdbiosciences.com/pharmingen/protocols/Biotinylated_Antibodies.shtml

Category: Protein Protocols: Western Blot Protocol

Detailed protocol for Western Blot using Biotinylated Antibodies. (BD PharMingen). - [Read Western Blotting with Biotinylated Antibodies]

Articles (1-3 of 3)

Probe Preparation for 22 x 40 mm DNA Microarrays

Probe Preparation for 22 x 40 mm DNA Microarrays Protocol.

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol. This protocol contains the steps for 3' end rapid amplification of mRNA by PCR. The first-strand cDNA is synthesized from total or poly(A+) RNA by priming from the poly-A tail of the mRNA using a oligo (dT) adaptor primer. The cDNA is then amplified via PCR using a gene-specific primer and an adaptor primer.