block Protocols

Search results for: block

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

ELISPOT Protocol Detailed PDF - http://www.biolegend.com/images/Protocols/BioLegend%20ELISPOT%20protocol.pdf

Category: ELISA Protocols: ELISpot Protocols

Coat the Plate, Block the Plate, Add Detection Antibody, Solutions and buffers for ELISpot. Biolegend. - [Read ELISPOT Protocol Detailed PDF]

HISTOLOGY-RELATED PROTOCOLS Fixation and Frozen Block PDF - http://www.uchsc.edu/misc/diabetes/derc/protocols/histologyprotocols.pdf

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Protocols for cell fixation and tissue fixation. HISTOLOGY-RELATED PROTOCOLS Fixation and Frozen Block techniques. Embedding Tissue Into Frozen Blocks. Fixing Cells with Fresh Paraformaldehyde. Preparation of Frozen Sections. Immunohistochemistry. Paraffin sections and Frozen sections. DERC Univ. Colorado PDF. - [Read HISTOLOGY-RELATED PROTOCOLS Fixation and Frozen Block PDF]

Immunostaining Conditions - http://www.bcm.edu/rosenlab/protocols/IHC_conditions.pdf

Category: Immunohistochemistry Protocols

Immunostaining conditions. Includes: Antibody, Host, Source, Cat #, Antigen Retrieval, Block, Diluent, Ab Dilution and 2° antibody. - [Read Immunostaining Conditions]

Preparing a cell block from cell lines / cell suspensions - http://icg.cpmc.columbia.edu/cattoretti/Protocol/Immunohistochemistry/Cell_block.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Preparing a cell block from cell lines / cell suspensions. Cattoretti, Colombia University. - [Read Preparing a cell block from cell lines / cell suspensions]

Preparing a Cell Block from Cell Lines Cell Suspensions Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/Cell_block.html

Category: Immunohistochemistry Protocols: Blocking Protocols

Protocol for preparing a cell block from cell lines / cell suspensions. Includes: Live cell preparation; Fixed cell preparation. - [Read Preparing a Cell Block from Cell Lines Cell Suspensions Protocol]

Resin Processing of Bone Protocol - http://www.nottingham.ac.uk/pathology/protocols/resbonecut.html

Category: Immunohistochemistry Protocols: Resin Staining Protocols

Protocol for resin processing of bone. Includes: FIXATION; PROCESSING; EMBEDDING; RELEASING BLOCK FROM GLASS CONTAINER; SECTIONING. - [Read Resin Processing of Bone Protocol]

SNP Genotyping System - http://www.promega.com/tbs/tm053/tm053.pdf

Category: Genetics and Genomics: Genotyping Protocols

SNP Genotyping System. Includes: Protocol for Manual READIT® Assay in Multiwell Plates; Preparation of L/L Reagent; Preparation of Heating Block; CIAP/Exo Treatment; Preparation of Interrogation Probe Solutions; Template Denaturation; Sample Interrogation; Protocol for Manual Plate Reading Luminometers; Protocol for Injecting Plate Reading Luminometers; Protocol for Automated READIT® Assay with the Biomek® 2000 Instrument. - [Read SNP Genotyping System]

Unmasking Hidden Epitopes Using the Microwave Oven Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4529

Category: Immunohistochemistry Protocols: Antigen Retrieval Protocols

Several methods have been developed to "retrieve" antigens that have been masked by fixation. The principle behind using the microwave oven method described here is to use extended periods of heat to break some of the subcellular structures that block antibody access. Be aware that any of the antigen retrieval methods should be avoided wherever possible, because they may introduce artifactual false-positive staining. - [Read Unmasking Hidden Epitopes Using the Microwave Oven Protocol]

Unmasking Hidden Epitopes Using the Pressure Cooker Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4530

Category: Immunohistochemistry Protocols: Antigen Retrieval Protocols

The principle behind the pressure cooker method described here is to use extended periods of heat to break some of the subcellular structures that block antibody access. This approach is appropriate for handling specimens on glass slides. The major advantages of the pressure cooker method are the ability to handle a large number of slides simultaneously, the convenience of using metal racks, and the avoidance of any hot spots that are found in the microwave. - [Read Unmasking Hidden Epitopes Using the Pressure Cooker Protocol]

Articles (1-2 of 2)

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Paraffin Embedding Protocol

Paraffin Embedding Protocol for molecular profiling. This Paraffin Embedding Protocol describes the processing of the tissues into sections following ethanol fixation. Molecular profiling (MP) is a technique that is used to visualize the global patterns of RNA expression or protein expression in various cell types and disease processes.

[1-2]