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A simple method for estimating global DNA methylation using bisulfite PCR of repetitive DNA elements - http://nar.oxfordjournals.org/cgi/content/full/32/3/e38

Category: Epigenetics and Methylation Protocols: Bisulfite Treatment Methylation Assay Protocol

Yang et al, NAR. 2004. ue to the heavy methylation of repetitive elements, this assay was especially useful in detecting decreases in DNA methylation, and this assay was validated by examining cell lines treated with the methylation inhibitor 5-aza-2'deox - [Read A simple method for estimating global DNA methylation using bisulfite PCR of repetitive DNA elements]

Bisulfite Treatment of DNA Anderson Lab - http://www.mdanderson.org/departments/methylation/display.cfm?id=955D5740-37D4-444F-ACF0193F8EC3989B&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: Epigenetics and Methylation Protocols

Bisulfite Treatment of DNA Anderson Lab. Adapted from Frommer et.al. - [Read Bisulfite Treatment of DNA Anderson Lab]

Bisulfite Treatment of DNA for Methylation Analysis - http://www.mdanderson.org/departments/methylation/display.cfm?id=955D5740-37D4-444F-ACF0193F8EC3989B&method=displayFull

Category: Epigenetics and Methylation Protocols: Bisulfite Treatment Methylation Assay Protocol

Adapted from Frommer et.al. Good protocol for bisulfite treatment of DNA. Includes tips on Methylation PCR for CpG methylation analysis. University of Texas M. D. Anderson Cancer Center - [Read Bisulfite Treatment of DNA for Methylation Analysis]

Bisulfite-PCR for Restriction Analysis and/or Sequencing - http://www.mdanderson.org/departments/methylation/display.cfm?id=8E0AAAAC-FA42-486C-A93F60CD6E1C7E51&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: Epigenetics and Methylation Protocols

Bisulfite-PCR for Restriction Analysis and/or Sequencing. Protocol written by Jean-Pierre Issa. M.D Anderson Cancer Centre Texas. - [Read Bisulfite-PCR for Restriction Analysis and/or Sequencing]

Bisulfite-PCR for Restriction Analysis and/or Sequencing Protocol - http://www.mdanderson.org/departments/methylation/display.cfm?id=8E0AAAAC-FA42-486C-A93F60CD6E1C7E51&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: PCR Protocols: Bisulfite Conversion Based PCR Protocols

Protocol for bisulfite-PCR for restriction analysis and/or sequencing. Bisulfite-PCR followed by restriction is a rapid and semi-quantitative method of analyzing DNA methylation. The PCR products are also suitable for either direct sequencing or cloning and sequencing. The most important step here is primer selection. - [Read Bisulfite-PCR for Restriction Analysis and/or Sequencing Protocol]

Fan Lab Bisulfite Treatment of Genomic DNA - http://www.genetics.ucla.edu/labs/fan/Protocols_Bisulfite.htm

Category: Epigenetics and Methylation Protocols: Bisulfite Treatment Methylation Assay Protocol

A detailed protocol for bisulfite treatment of DNA. DNA is first digested with a restriction enzyme. DNA is denauted at 97C for 5min. Bisulfite protocol is included. Also DNA purification using the Promega Wizard DNA Cleanup kit in indicated. Fan Lab. UCL - [Read Fan Lab Bisulfite Treatment of Genomic DNA]

Hairpin-bisulfite PCR: Assessing epigenetic - http://staff.washington.edu/miner/hpbspcr/Laird%20et%20al%202004.pdf

Category: Epigenetics and Methylation Protocols: Bisulfite Treatment Methylation Assay Protocol

Hairpin-bisulfite PCR will be a powerful tool in studying other processes for which genetic or epigenetic information differs on the two complementary strands of DNA. Laird et al., 2003. PNAS - [Read Hairpin-bisulfite PCR: Assessing epigenetic]

Quantification of DNA Methylation in Electrofluidics Chips Protocol - http://www.natureprotocols.com/2006/06/23/quantification_of_dna_methylat.php

Category: Nucleic Acid Modification Protocols: DNA Methylation Protocols

Protocol for quantification of DNA methylation in electrofluidics chips. Describe Bio-COBRA, a modified protocol for Combined Bisulfite Restriction Analysis (COBRA), that incorporates an electrophoresis step in microfluidics chips. Microfluidics technology involves the handling of small amounts of liquid in miniaturized systems. - [Read Quantification of DNA Methylation in Electrofluidics Chips Protocol]