biotinylated Protocols

Category: Immunohistochemistry Protocols: Antigen Retrieval Protocols

Protocol used to for immunohistochemistry on paraffin-embedded sections. Based on use of microwave energy to effect antigen retrieval. The immunohistochemistry procedure, is for use of Biomeda's HistoScan kit based on a streptavidin-peroxidase/biotinylated second antibody detection system with 3-amino, 9-ethylcarbazole (AEC) as chromogen. Undoubtedly, other kits or home-made reagents will also work . - [Read Antigen Retrieval for Immunohistochemistry with Paraffin-Embedded Tissues Protocol]

Category: Antibody Protocols: Antibody Labeling Protocols

This protocol describes the covalent coupling of antibodies to biotin. Biotin groups bind with extremely high affinity to streptavidin and avidin, both of which are available commercially coupled with enzymes, fluorescent dyes, or iodine. A biotinylated primary antibody, therefore, can be detected with any of a wide variety of different labels. The biotinylation reaction is simple and mild, and rarely inactivates the antibody. - [Read Labeling Antibodies with Biotin Protocol]

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Single colour detection for biotinylated probes. Sanger Institute - [Read Single Colour Fish Analysis Method]

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Two-colour detection for combinations of biotinylated and digoxigenin-labelled probes. Sanger Institute - [Read Two-colour detection for combinations of biotinylated and digoxigenin-labelled probes]

Category: Protein Protocols: Western Blot Protocol

Detailed protocol for Western Blot using Biotinylated Antibodies. (BD PharMingen). - [Read Western Blotting with Biotinylated Antibodies]