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Agarose Gel Preparation Pictures - http://www.biotech.iastate.edu/publications/ppt_presentations/html/Fingerprinting/AgaroseGelPrep/02.html

Category: General Research Protocols and Methods

Agarose Gel Preparation Pictures. Mike Zeller - [Read Agarose Gel Preparation Pictures]

Basic Method for Direct Immunofluorescence Labeling - http://www.biotech.iastate.edu/facilities/CELLHYB/Labeling.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Basic Method for Direct Immunofluorescence Labeling. Iowa State University, Flow Cytometry Facility. - [Read Basic Method for Direct Immunofluorescence Labeling]

Factors that Influence Restriction Enzyme Digestion Activity - http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/enzymes/cuteffects.html

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

Factors that Influence Restriction Enzyme Digestion Activity. Buffer Composition, Incubation Temperature, DNA Methylation, Star Activity, Multiple Enzymes. Laura Austgen PhD et. al, Biomedical Hypertextbook, Colorado State Univ. - [Read Factors that Influence Restriction Enzyme Digestion Activity]

FIXATION and DNA Staining for Cell Cycle Analysis - http://www.biotech.iastate.edu/facilities/CELLHYB/CCAnalysis.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

FIXATION and DNA Staining for Cell Cycle Analysis Protocol. This method of DNA staining utilizes ethanol to fix the cells and permeabilize the membrane, which allows the dye (Propidium Iodide) to enter the cells. Propidium Iodide (PI) is a DNA-binding fluorochrome that intercalates in the double-helix. Ribonuclease-A is used to eliminate the staining of double-stranded RNA. - [Read FIXATION and DNA Staining for Cell Cycle Analysis]

Indirect Immunofluorescence Labeling Protocol - http://www.biotech.iastate.edu/facilities/CELLHYB/Indirect.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

An Indirect Immunofluorescence Labeling Protocol for flow cytometry. Iowa State University, Flow Cytometry Facility - [Read Indirect Immunofluorescence Labeling Protocol]

KickStartâ„¢ Protein Expression Kit PDF - http://www.dualsystems.com/pdf/KickStart/P01006_KickStart.pdf

Category: Protein Protocols: Protein Expression Protocols

KickStartâ„¢ Protein Expression Kit PDF. Dual Systems Biotech - [Read KickStartâ„¢ Protein Expression Kit PDF]

Paraformaldehyde Fixation of Cells - http://www.biotech.iastate.edu/facilities/CELLHYB/Fixation.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Paraformaldehyde Fixation of Cells protocol. This fixation method is good for cells labeled by fluorochrome-conjugated antibodies to membrane antigens. It will stabilize the light scatter and labeling for up to a week in most instances, allowing you to be more flexible in scheduling cytometer time. Furthermore, it inactivates most biohazardous agents, so it is important from a safety standpoint as well. Iowa University. - [Read Paraformaldehyde Fixation of Cells]

Plasmid Isolation and Analysis. Pictorial Protocol. - http://www.biotech.iastate.edu/publications/ppt_presentations/html/IsoPart1_files/v3_document.htm

Category: General Research Protocols and Methods

Plasmid Isolation and Analysis. Pictorial Protocol. Mike Zeller. - [Read Plasmid Isolation and Analysis. Pictorial Protocol.]

Protocol for Manual Peptide Synthesis - http://www.biotech.unl.edu/proteomics/protocol.html

Category: Peptide Protocols

Protocol for Manual Peptide Synthesis. Resin Swelling And Coupling Of Activated Amino Acid Esters. Deblocking and cleaving peptide from solid support. Synthesis of Peptides. The University of Nebraska-Lincoln Protein Core Facility - [Read Protocol for Manual Peptide Synthesis]

Protocol for Protein Extraction Using Proteomics - http://www.biotech.unl.edu/proteomics/mercaptoethanol.html

Category: Protein Protocols: Protein Extraction Protocols

Protocol for Protein Extraction Using Proteomics. Extraction of proteins from plant cells that are rich in compounds that interfere with the 2-Dimensional electrophoretic separation methods such as salts, organic acids, phenolics, pigments, terpenes, among others. A common protocol used in our lab for extraction proteins from plant tissues consists in the homogenization of mortar-grounded material in liquid nitrogen with an extraction buffer. - [Read Protocol for Protein Extraction Using Proteomics]

Quantitative Flow Cytometry for Apoptosis - http://www.biotech.uiuc.edu/centers/Proteomics/Flow_cytometry/docs/Apopbroc.pdf

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Quantitative Flow Cytometry for Apoptosis Detection. Flow Cytometry Facility, U.Illinois. - [Read Quantitative Flow Cytometry for Apoptosis]

Soybean Cotyledonary-node Agrobacterium-mediated Transformation System - http://www.biotech.unl.edu/transgenic/protocols.html

Category: Plant Biology Protocols

Protocol includes: Seed Sterilization Step; Seed Germination Step; Agrobacterium Inoculum Preparation; Explant Preparation; Co-cultivation Plates; Shoot Initiation Step; Shoot Elongation Step; Rooting Step. - [Read Soybean Cotyledonary-node Agrobacterium-mediated Transformation System]

Step by Step Lab Protocols using pictures - http://www.biotech.iastate.edu/publications/ppt_presentations/default.html

Category: General Research Protocols and Methods

Step by Step Lab Protocols using pictures. Mike Zeller. - [Read Step by Step Lab Protocols using pictures]

Use of the Lab Micropipette - http://www.biotech.iastate.edu/publications/ppt_presentations/html/DNAMicropipetteUseage/01.html

Category: General Research Protocols and Methods

Use of the Lab Micropipette. Mike Zeller - [Read Use of the Lab Micropipette]