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A Method for Assaying Deubiquitinating Enzymes - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=140113

Category: Protein Protocols: Protein Ubiquitination Protocols

A Method for Assaying Deubiquitinating Enzymes. Lee et al., Biol Proced Online. May 1998. A general method for the assay of deubiquitinating enzymes was described in detail using 125I-labeled ubiquitin-fused Î±NH-MHISPPEPESEEEEEHYC (referred to as Ub-PESTc) as a substrate. - [Read A Method for Assaying Deubiquitinating Enzymes]

Large scale nuclear extract preparation - http://mesentsev.narod.ru/Biblioteque/Hattori.html

Category: Protein Protocols: Nuclear Extraction Protocols

Homogenize cells or tissue, Pellet nuclei, Lyse nuclei, Reprecipitate nuclear proteins, dialyze nuclear extract. Hattori M et al., DNA Cell Biol. 1990. Homogenization buffer and dialysis buffer recipes. - [Read Large scale nuclear extract preparation]

Lowry Protein Assay - http://www.animal.ufl.edu/hansen/protocols/LOWRY.htm

Category: Protein Protocols: Lowry Protein Assay

Lowry Protein Assay. The Lowry procedure is one of the most venerable and widely-used protein assays, being first described in 1951 [Lowry et al., J. Biol. Chem. 193: 265-275 (1951)]. Under alkaline conditions, copper complexes with protein. When folin phenol reagent (phospho-molybdic-phosphotungstic reagent) is added, the Folin-phenol reagent binds to the protein. Bound reagent is slowly reduced and changes color from yellow to blue. P.J. Hansen, Dept. of Animal Sciences, University of Florida. - [Read Lowry Protein Assay]

Restriction Enzyme Digestion of DNA - http://www.facstaff.bucknell.edu/pizzorno/327-lab4.html

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

Digesting DNA with Restriction Endonucleases introduction. Analytical and Preparative reaction mixes. All about restriction enzymes. Pizzorno, BIOL 327. - [Read Restriction Enzyme Digestion of DNA]

Articles (1-1 of 1)

DNA Ligation Protocol

The DNA Ligation protocol described here contains the steps required to join together using ligase enzyme both plasmid DNA and insert DNA fragments in order to create a new plasmid. This new ligated plasmid can be transformed after into competent bacteria to produce DNA for mini, midi or maxi-prep isolation.