User Panel

My Panel

Bookmark Science Articles

biochem Protocols

Search results for: biochem

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

2 Hybrid System TRAFO Protocol - http://www.umanitoba.ca/faculties/medicine/biochem/gietz/2HS.html

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

Yeast Two-Hybrid System. TRAFO Solutions. Gietz Lab - [Read 2 Hybrid System TRAFO Protocol]

Aggregation of ES cells and eight cell stage embryos - http://web.archive.org/web/20021020163836/grimwade.biochem.unimelb.edu.au/bowtell/cellbiol/sect65.htm#Aggregation%20of%20ES%20cells%20and%20eight%20cell%20stage%20embryos

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Aggregation of ES cells and eight cell stage embryos. Bowtell Lab. - [Read Aggregation of ES cells and eight cell stage embryos]

Apoptosis Detection: Annexin V + 7-AAD Protocol - http://biochem.dental.upenn.edu/~sdmfacs/apopproc.htm#annexin

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Apoptosis Cytometry Protocols

Apoptosis detection using the protocol annexin V + 7-AAD. - [Read Apoptosis Detection: Annexin V + 7-AAD Protocol]

Apoptosis Detection: Hoechst vs. 7-AAD Protocol - http://biochem.dental.upenn.edu/~sdmfacs/apopproc.htm#33258

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Apoptosis Cytometry Protocols

Protocol for apoptosis detection using Hoechst vs. 7-AAD protocol. - [Read Apoptosis Detection: Hoechst vs. 7-AAD Protocol]

Apoptosis Detection: TUNEL Protocol - http://biochem.dental.upenn.edu/~sdmfacs/apopproc.htm#brduapop

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Apoptosis Cytometry Protocols

Protocol for apoptosis detection using TUNEL protocol. This technique uses the enzyme terminal deoxynucleotidyl transferase (TdT) to label cells that have oligonucleosomal nicks/strand breaks in their DNA. - [Read Apoptosis Detection: TUNEL Protocol]

ß- Galactosidase Assay Protocol - http://www.biochem.northwestern.edu/ibis/morimoto/research/Protocols/VI.%20Transcription/I.%20b-gal%20.pdf

Category: Yeast Protocols: Yeast Genetics Protocols: B-Galactosidase Assay Protocols

Protocol for ß- Galactosidase assay. - [Read ß- Galactosidase Assay Protocol]

B- Galactosidase Assay Protocol - http://www.biochem.northwestern.edu/ibis/morimoto/research/Protocols/VI.%20Transcription/I.%20b-gal%20.pdf

Category: Reporter Gene Assays: B-Galactosidase Assay Protocols

The recommended amount of RSV-ß-Galactosidase plasmid to use for transfection of cells (60 mm or 100 mm dish) is 1-2 µg. The optimal amount of plasmid DNA will be determined by the efficiency of transfection , which is very dependent upon the particular cell line and transfection protocol. - [Read B- Galactosidase Assay Protocol]

Blastocyst Transfer. - http://web.archive.org/web/20021215221246/grimwade.biochem.unimelb.edu.au/bowtell/cellbiol/sect66.htm#BLASTOCYST%20TRANSFER

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Blastocyst Transfer. Bowtell Lab. - [Read Blastocyst Transfer.]

C. elegans Integration via Gamma Radiation Protocol - http://www.biochem.northwestern.edu/ibis/morimoto/research/Protocols/IX.%20C.%20elegans/E.%20Genetics/1.%20Integration%20via%20gamma.pdf

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol for C. elegans integration via gamma radiation. - [Read C. elegans Integration via Gamma Radiation Protocol]

CAT Assay Protocols - http://www.biochem.northwestern.edu/ibis/morimoto/research/Protocols/VI.%20Transcription/G.%20CAT%20ASSAY.pdf

Category: Reporter Gene Assays: Cat Assays Protocols

Two protocols describing CAT assays. - [Read CAT Assay Protocols]

Cohesive End Ligation - http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/protocols/ligation.html

Category: Molecular Biology Protocols: DNA Ligation Protocols

DNA ligation protocol with excellent notes and tips. Chen Lab, Univ. College London. - [Read Cohesive End Ligation]

Column Chromatography Protocol - http://www.biochem.arizona.edu/classes/bioc463a/Info/protocols/exp_4_1.pdf

Category: Chromatography Protocols: Column Chromatography Protocols

Size Exclusion Column Chromatography Protocol. PDF. In this protocol you will learn how to use three types of column chromatography: Gel Filtration or Size Exclusion (SEC), Ion Exchange (IEC), and affinity (AC) in order to purify proteins and enzymes based on the physical properties of these biomolecules. Univ. Arizona, Biochemistry. - [Read Column Chromatography Protocol]

Complex Yeast Media Protocols - http://www.umanitoba.ca/faculties/medicine/biochem/gietz/media.html

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Complex yeast media protocols. Including: YPAD Medium or Also Called YPD plus Adenine; Synthetic Complete Drop Out (SC drop-out)Medium; Synthetic Complete drop-out Medium Mix (SC drop-out). - [Read Complex Yeast Media Protocols]

Dual Luciferase Reporter System Protocol (PROMEGA) - http://www.biochem.northwestern.edu/ibis/morimoto/research/Protocols/II.%20Eukaryotes/F.%20Luciferase%20Assays/2b.Dual%20luciferase.pdf

Category: Reporter Gene Assays: Luciferase Assay Protocols

Protocol for dual luciferase reporter system. Includes: Transfections; Cell Lysis; Reagent Preparation; Luminescence measurement; Wash injectors. - [Read Dual Luciferase Reporter System Protocol (PROMEGA)]

Expression of F-tryptophan labelled protein - http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/protocols/fluoexp.html

Category: Protein Protocols: Protein Expression Protocols

Expression of F-tryptophan labelled protein. Protocol can be adapted for Fluoro-phenylalanine or Fluro-tyrosine labelling. Transformation of competent E. coli cells for expression with plasmid. Dr. Chen, Dept. of Biochem. & Mol. Biology, University College, London. - [Read Expression of F-tryptophan labelled protein]

Expression of protein in minimal media - http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/protocols/protexp.html

Category: Protein Protocols: Protein Expression Protocols

Expression of protein in minimal media using E. coli cells for expression. Dr. Chen, Biochem. & Mol. Biology, University College. London - [Read Expression of protein in minimal media]

FACS Procedures for Apoptosis Detection TUNEL - http://biochem.dental.upenn.edu/~sdmfacs/apopproc.htm

Category: Cell Biology and Cell Culture: Apoptosis Protocols: TUNEL assay apoptosis

FACS Procedures for Apoptosis Detection with TUNEL. U. Pennsylvania Dental School. - [Read FACS Procedures for Apoptosis Detection TUNEL]

Frozen Yeast Transformation Protocol - http://www.umanitoba.ca/faculties/medicine/biochem/gietz/Frozen.html

Category: Yeast Protocols

This Protocol allows You to prepare Frozen Yeast cells that are competent for transformation after thawing. - [Read Frozen Yeast Transformation Protocol]

Gel Mobility Shift Assay in PDF format - http://www.biochem.northwestern.edu/ibis/morimoto/Protocols/III.%20Proteins/C.%20DNA-Protein%20Interactions/3a.%20GMSA.pdf

Category: DNA Protocols: EMSA DNA-Protein Protocols

This protocol works well for HSF DNA-binding activity experiments. - [Read Gel Mobility Shift Assay in PDF format]

Gel Mobility Shift Assay Protocol - http://www.biochem.northwestern.edu/ibis/morimoto/research/Protocols/III.%20Proteins/C.%20DNA-Protein%20Interactions/3a.%20GMSA.pdf

Category: DNA Protein Interactions Protocols: Electrophoretic Mobility Shift Assays EMSA Protocols

Protocol for gel mobility shift assay. - [Read Gel Mobility Shift Assay Protocol]

Gel Super-Shift Assay EMSA Protocol - http://www.biochem.northwestern.edu/ibis/morimoto/research/Protocols/III.%20Proteins/C.%20DNA-Protein%20Interactions/3b.%20Super-shift.pdf

Category: DNA Protein Interactions Protocols: Electrophoretic Mobility Shift Assays EMSA Protocols

Protocol for gel super shift assay EMSA. - [Read Gel Super-Shift Assay EMSA Protocol]

Gold labeling of proteins for electron microscopy - http://www.biochem.wisc.edu/inman/empics/gold.htm

Category: Molecular Imaging: Electron Microscopy Protocols

In brief, the reaction mixture, is prepared in exactly the same way as if the sample is to be stained or shadowed for electron microscopy, but once the... Gold labeling technique for electron microscopic identification and location of proteins. - [Read Gold labeling of proteins for electron microscopy]

High Efficiency Transformation Protocol - http://www.umanitoba.ca/faculties/medicine/biochem/gietz/method.html

Category: Yeast Protocols: Yeast Transformation Protocols: High Efficiency Yeast Transformation Protocols

Protocol for high efficiency transformation. - [Read High Efficiency Transformation Protocol]

High-Efficiency LiOAc Transformation of S. cerevisiae Protocol - http://www.biochem.emory.edu/labs/acorbe2/yeasttransformation.html

Category: Yeast Protocols: Yeast Transformation Protocols: High Efficiency Yeast Transformation Protocols

Protocol for high-efficiency LiOAc transformation of S. cerevisiae. - [Read High-Efficiency LiOAc Transformation of S. cerevisiae Protocol]

Immunofluorescence Protocol - http://www.biochem.emory.edu/labs/acorbe2/immuno.html

Category: Immunology: Immunofluorescence Protocols

Protocol for immunofluorescence. - [Read Immunofluorescence Protocol]

Articles (1-1 of 1)

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

Items 1-25 out of 52 displayed.

First [1] 2 3 Next Last

Total records: 52