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Isolation of Yeast Mitochondria in aPre-Formed Iodixanol Gradient - http://www.axis-shield.com/densityhome/optiprep/S27.pdf

Category: Cell Organelle Protocols: Subcellular Fractionation Protocols: Mitochondria Isolation Protocols

A discontinuous gradient of iodixanol is used in which the crude mitochondrial fraction is layered beneath the preformed gradient. In this protocol the osmolality of the gradient is approx 600 mOsm and the crude mitochondrial fraction is loaded in 40% iodixanol rather than 50% iodixanol. - [Read Isolation of Yeast Mitochondria in aPre-Formed Iodixanol Gradient]

Purification of viable bovine spermatozoa of normal morphology. - http://www.axis-shield.com/densityhome/optiprep/C16.pdf

Category: Cell Biology and Cell Culture

Protocol for the recovery of a highly viable fraction for use in fertilization. Protocol uses an iodinated density gradient medium to adjust the density of the raw ejaculate to approx 1.170 g/ml by mixing with a high density medium (Ï >1.26 g/ml) and loading it beneath a discontinuous gradient. - [Read Purification of viable bovine spermatozoa of normal morphology.]