behavior Protocols

Category: Cell Culture Protocols: Sterile Technique Protocols

Cultured mammalian cells are used extensively in cell biology studies; it requires a number of special skills in order to be able to preserve the structure, function, behavior and biology of the cells. This unit describes the basic skills required to maintain and preserve cell cultures: aseptic technique, medium characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells. - [Read Basic Techniques for Mammalian Cell Tissue Culture Protocol]

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

Cultured mammalian cells are used extensively in cell biology studies; it requires a number of special skills in order to be able to preserve the structure, function, behavior and biology of the cells. This unit describes the basic skills required to maintain and preserve cell cultures: aseptic technique, medium characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells. - [Read Basic Techniques for Mammalian Cell Tissue Culture Protocol]

Category: Animal Research Techniques

Guinea Pig Biology, Sources Ordering, Behavior, Basic Husbandry, Identification, Handling, Breeding, Restraint Devices, Sexing, Transport, Anesthesia, Euthanasia, Rodent Surgery, Blood Collection, Administration of Fluid. Univ. Iowa. Michael Parker. - [Read Biomethodology of the Guinea Pig]

Category: Animal Research Techniques

Mus mouse Biology, Sources Ordering, Behavior, Basic Husbandry, Identification, Handling, Breeding, Restraint Devices, Sexing, Transport, Anesthesia, Euthanasia, Rodent Surgery, Blood Collection, Administration of Fluid. Univ. Iowa. Michael Parker. - [Read Biomethodology of the Mouse]

Category: Animal Research Techniques

Rabbit Biology, Sources Ordering, Behavior, Basic Husbandry, Identification, Handling, Breeding, Restraint Devices, Sexing, Transport, Anesthesia, Euthanasia, Rodent Surgery, Blood Collection, Administration of Fluid. Univ. Iowa. Michael Parker. - [Read Biomethodology of the Rabbit]

Category: Animal Research Techniques

Rat Biology, Sources Ordering, Behavior, Basic Husbandry, Identification, Handling, Breeding, Restraint Devices, Sexing, Transport, Anesthesia, Euthanasia, Rodent Surgery, Blood Collection, Administration of Fluid. Univ. Iowa. Michael Parker. - [Read Biomethodology of the Rat]

Category: Microscopy Protocols: Live-Cell Imaging Protocols

Using confocal laser-scanning microscope & GFP fusion proteins in time-lapse imaging to visualize the behavior of organelles and to track membrane-bound transport intermediates that bud off from organelles. Practical issues related to construction & expression of GFP fusion proteins are discussed. Essential for optimizing the brightness and expression levels of GFP fusion proteins so that intracellular membrane-bound structures containing these fusion proteins can be readily visualized. - [Read Imaging of Organelle Membrane Systems and Membrane Traffic in Living Cells]

Category: Microinjection Protocols: Microinjection of Proteins Protocols

Activation and inactivation of proteins using photoactivation of caged peptides or proteins offer insights into cellular dynamics not achievable using genetic means. The ability to selectively alter the activity of a specific protein at a defined time and location inside a cell allows the correlation of changes in protein activity and cellular behavior. A caged compound, peptide, or protein is prepared by covalently linking it to a photolabile, protecting group. - [Read Introduction of Caged Peptide/Protein into Cells Using Microinjection Protocol]