bead Protocols

Category: RNA Protocols: RNA-binding Protein Purification

Affinity Chromotography Magnetic Bead Purification of RNA-binding proteins from Rat Brain. Scaturro et al. 2003. - [Read Affinity Chromotography Magnetic Bead Purification of RNA-binding proteins from Rat Brain.]

Category: Neuroscience Protocols

Bead aggregation assay to demonstrate presynaptic differentiation induced by the NGL family of cell adhesion molecules. - [Read Bead Aggregation Assay Protocol]

Category: Epigenetics and Methylation Protocols: ChIP Chromatin Immunoprecipitation Protocols

Chromatin Immunoprecipitation Protocol for Microarray Analysis – Protein A/G Bead Method. UHN Microarray Centre. Brown and Farnham labs by James Paris and Mark Takahashi. - [Read Chromatin Immunoprecipitation Protocol]

Category: DNA Microarray Protocols

Chromatin Immunoprecipitation Protocol for Microarray Analysis – Protein A/G Bead Method. Protocol From the UHN Microarray Centre - [Read Chromatin Immunoprecipitation Protocol for Microarray Analysis]

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

Chromatin Immunoprecipitation Protocol for Microarray Analysis – Protein A/G Bead Method. Cross-Linking, Sonication/Chromatin Shearing Efficiency. Reaction UHN Microarray Centre - [Read Chromatin Immunoprecipitation Protocol PDF]

Category: RNA Protocols: RNA-binding Protein Purification

Dynal Bead RNA-Binding Protein Purification. DYNAL. - [Read Dynal Bead RNA-Binding Protein Purification]

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Sorting Protocols

Questions and answers about cell sorting. Includes: When should I use fluorescence activated cell sorting over bulk separation methods like panning or magnetic bead separations? Will my cells be harmed by the sorting process? How many cells do I need to prepare to recover 1 X 106 of a population that comprises 10% of the cells? Are there ways to improve sort recovery? etc... - [Read FAQs About Cell Sorting]

Category: Immunology: Mononuclear Cell Isolation Protocols: B Lymphocyte Isolation Protocols

Protocol describes the purification of mouse T cells, B cells, and T cell subsets using magnetic bead separation. Isolation of cell subsets using magnetic beads is quick, simple, and reliable and can result in high yields of very pure cells. - [Read Fractionation of T and B Cells Using Magnetic Beads Protocol]

Category: Immunology: Mononuclear Cell Isolation Protocols: T-Cell Isolation Protocols

Protocol describes the purification of mouse T cells, B cells, and T cell subsets using magnetic bead separation. Isolation of cell subsets using magnetic beads is quick, simple, and reliable and can result in high yields of very pure cells. - [Read Fractionation of T and B Cells Using Magnetic Beads Protocol]

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

GST Fusion Protein Purification from Yeast. Lysis Buffer recipe for yeast, glutathione bead method for GST purification. Dohlman Lab - [Read GST Fusion Protein Purification from Yeast]

Category: Chromatography Protocols

His Tag Nickel Affinity Chromatography Protocol PDF. The Wallert and Provost Lab. Theory and Introduction: Ni-Affinity Chromatography uses the ability of His to bind nickel. Six histadine amino acids at the end of a protein (either N or C terminus) is known as a 6X His tag. Nickel is bound to an agarose bead by chelation using nitroloacetic acid (NTA) beads. Several companies produce these beads as His Tagged proteins are some of the most used affinity tags in today’s market. - [Read His Tag Nickel Affinity Chromatography Protocol PDF]