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A Batch Test Tube Method for the Calculation of an Adsorbent’s Available Capacity Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4212

Category: Chromatography Protocols: Affinity Chromatography Protocols

Protocol describes a batch test tube method for the calculation of an adsorbent’s available capacity. - [Read A Batch Test Tube Method for the Calculation of an Adsorbent’s Available Capacity Protocol]

Purification of Histidine-tagged Proteins by Immobilized Ni2+ Absorption Chromatography Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4088

Category: Chromatography Protocols: Affinity Chromatography Protocols

Recombinant proteins engineered to have a polyhistidine tail at either the carboxyl or amino terminus can easily be purified in one step by affinity chromatography on a resin carrying chelated nickel ions. Chromatography can be carried out in column or batch formats. After unbound proteins are washed away, the target protein is eluted using imidazole, which typically preserves the antigenic and functional features of the protein. - [Read Purification of Histidine-tagged Proteins by Immobilized Ni2+ Absorption Chromatography Protocol]

Selection of Poly(A)+ RNA by Batch Chromatography - Subscription Required - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4048

Category: Chromatography Protocols: DNA RNA Affinity Chromatography

When many RNA samples are to be processed or when working with small amounts (<50 Âµg) of total mammalian RNA, the technique of choice is batch chromatography on oligo(dT)-cellulose. The method described in this protocol uses a combination of temperature and ionic strength to maximize binding and recovery of polyadenylated RNA. IMPORTANT: Prepare all reagents used in this protocol with Diethyl pyrocarbonate (DEPC)-treated H2O. Joseph Sambrook and David W. Russell. - [Read Selection of Poly(A)+ RNA by Batch Chromatography - Subscription Required]

Selection of Poly(A)+ RNA by Oligo(dT)-Cellulose Chromatography - Subscription Required - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4047

Category: Chromatography Protocols: DNA RNA Affinity Chromatography

Chromatography on oligo(dT) columns is the preferred method for large-scale purification (>25 Âµg) of poly(A)+ RNA extracted from mammalian cells. Typically, between 1% and 10% of the RNA applied to the oligo(dT) column is recovered as poly(A)+ RNA. Because the method can be frustratingly slow, it is not recommended for purification of poly(A)+ RNA from multiple samples. For this purpose, batch elution (Selection of Poly(A)+ RNA by Batch Chromatography) is the better choice. - [Read Selection of Poly(A)+ RNA by Oligo(dT)-Cellulose Chromatography - Subscription Required]