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Basic Information on In Vitro Transcription - http://www.ambion.com/techlib/basics/transcription/index.html

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

The ability to synthesize RNA in the lab is critical to many techniques.Radiolabeled and nonisotopically labeled RNA probes, generated in small scale transcription reactions can be used in blot hybridizations and nuclease protection assays. This article includes information on: Requirements For Transcription, RNA Phage Polymerases, Template Options: Plasmids, PCR Products, Oligonuclotides and cDNA, Sense or Antisense, Conventional Or Large Scale Synthesis, Products for In Vitro Transcription. - [Read Basic Information on In Vitro Transcription]

GENERAL USE OF ENZYMES IN MOLECULAR BIOLOGY - http://www.people.hofstra.edu/faculty/Beverly_Clendening/Adv_Molecular_Biology/Protocols/General_use_of_Enzymes.html

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

GENERAL USE OF ENZYMES IN MOLECULAR BIOLOGY. Basics of Restriction Enzymes. - [Read GENERAL USE OF ENZYMES IN MOLECULAR BIOLOGY]

Guide to Genetic Mapping in C. elegans - http://www.uwyo.edu/MolecBio/Fay/WBF_-_Super_Geniuses.pdf

Category: C. elegans Protocols: C. elegans Genetics Protocols

A guide to genetic mapping in C. elegans. Includes: Introduction and basics; Two-point mapping; Three-point mapping; Deficiency and duplication mapping; Basics of SNP mapping; Mapping dominant mutations; Mapping suppressor/enhance mutations; Mapping of synthetic mutations. - [Read Guide to Genetic Mapping in C. elegans]

Real Time PCR the Basics Ambion - http://www.ambion.com/techlib/basics/rtpcr/

Category: Real Time PCR

Real Time PCR the Basics Ambion - [Read Real Time PCR the Basics Ambion]

Real-Time PCR (qPCR) Basics - http://web.archive.org/web/20040711051207/http://primerdesign.co.uk/primerdesign/qPCR_basics.html

Category: Real Time PCR

Learn Quantitative PCR Methodology. Assay parameters, Melt Curves and Primer Dimers, Standard Curves, Dynamic Range, Quantification methods, Delta CT, Standard Curves. Dr.Powell. - [Read Real-Time PCR (qPCR) Basics]