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Assay of β-Galactosidase in Yeast Protocol - http://www.oardc.ohio-state.edu/stockingerlab/Protocols/BetaGalAssays.pdf

Category: Reporter Gene Assays: B-Galactosidase Assay Protocols

There are two basic methods for the in vitro assay of B-galactosidase activity from yeast. They differ mainly in the method of preparing the material for assay. Both methods are described with accompanying protocols. Method I: Assay of Crude Extracts includes: Yeast Cell Growth; Yeast Cell Harvest; B-gal assays; Bradford Assays. Method II: Permeabilized cell assay. - [Read Assay of β-Galactosidase in Yeast Protocol]

Basic Cell Culture Protocols - http://www.hyclone.com/library/basicprotocols.htm

Category: Cell Biology and Cell Culture

Basic Cell Culture Protocols. Passaging, Counting, Viability and more. Hyclone - [Read Basic Cell Culture Protocols]

Basic ELISA Method - http://iacf.bsd.uchicago.edu/Monoclonal_Antibody_Facility/BasicELISAProtocol.htm

Category: ELISA Protocols

Basic ELISA Method. All the steps for the basic ELISA protocol. Immunology Applications Core Facility, Univ. Chicago. - [Read Basic ELISA Method]

Basic Ideas in PCR Protocol Design. PDF - http://www.idtdna.com/support/technical/TechnicalBulletinPDF/A_Basic_PCR_Protocol.pdf

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

Basic Ideas in PCR Protocol Design. PDF - [Read Basic Ideas in PCR Protocol Design. PDF]

Basic Ideas in PCR Protocol Design. PDF - http://www.idtdna.com/support/technical/TechnicalBulletinPDF/A_Basic_PCR_Protocol.pdf

Category: PCR Protocols

Basic Ideas in PCR Protocol Design. PDF - [Read Basic Ideas in PCR Protocol Design. PDF]

Basic Information on In Vitro Transcription - http://www.ambion.com/techlib/basics/transcription/index.html

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

The ability to synthesize RNA in the lab is critical to many techniques.Radiolabeled and nonisotopically labeled RNA probes, generated in small scale transcription reactions can be used in blot hybridizations and nuclease protection assays. This article includes information on: Requirements For Transcription, RNA Phage Polymerases, Template Options: Plasmids, PCR Products, Oligonuclotides and cDNA, Sense or Antisense, Conventional Or Large Scale Synthesis, Products for In Vitro Transcription. - [Read Basic Information on In Vitro Transcription]

Basic Method for Direct Immunofluorescence Labeling - http://www.biotech.iastate.edu/facilities/CELLHYB/Labeling.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Basic Method for Direct Immunofluorescence Labeling. Iowa State University, Flow Cytometry Facility. - [Read Basic Method for Direct Immunofluorescence Labeling]

Basic Methods of Culturing Drosophila Protocol - http://fly.bio.indiana.edu/Fly_Work/culturing.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for the basic methods of culturing Drosophila. Includes: Stockkeeping; Culture contaminants; Experimental populations. - [Read Basic Methods of Culturing Drosophila Protocol]

Basic PCR Protocol - http://preuss.bsd.uchicago.edu/protocols/PCR.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

Basic PCR Protocol. (per Adam 08/12/04). - [Read Basic PCR Protocol]

Basic PCR Protocol - http://preuss.bsd.uchicago.edu/protocols/PCR.html

Category: PCR Protocols

Basic PCR Protocol. (per Adam 08/12/04). - [Read Basic PCR Protocol]

Basic Techniques for Mammalian Cell Tissue Culture Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662B5FE053DEE8CB4AC3EB6F729A11&objectid=667396C6F0D96C40EB68A3D8DB8B201A

Category: Cell Culture Protocols: Sterile Technique Protocols

Cultured mammalian cells are used extensively in cell biology studies; it requires a number of special skills in order to be able to preserve the structure, function, behavior and biology of the cells. This unit describes the basic skills required to maintain and preserve cell cultures: aseptic technique, medium characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells. - [Read Basic Techniques for Mammalian Cell Tissue Culture Protocol]

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

Beta-gal Assay Protocol - http://www.fhcrc.org/science/labs/gottschling/yeast/Bgal.html

Category: Reporter Gene Assays: B-Galactosidase Assay Protocols

Basic protocol for a Beta-gal assay. - [Read Beta-gal Assay Protocol]

BHK-21. Basic Cell Culture Protocols - http://www.sci.sdsu.edu/classes/chemistry/chem467l/mardahl/basiccell.html

Category: Cell Biology and Cell Culture

Basic Cell Culture Protocols. BHK-21. - [Read BHK-21. Basic Cell Culture Protocols]

Biomethodology of the Guinea Pig - http://research.uiowa.edu/animal/?get=g_pig

Category: Animal Research Techniques

Guinea Pig Biology, Sources Ordering, Behavior, Basic Husbandry, Identification, Handling, Breeding, Restraint Devices, Sexing, Transport, Anesthesia, Euthanasia, Rodent Surgery, Blood Collection, Administration of Fluid. Univ. Iowa. Michael Parker. - [Read Biomethodology of the Guinea Pig]

Biomethodology of the Mouse - http://research.uiowa.edu/animal/?get=mouse

Category: Animal Research Techniques

Mus mouse Biology, Sources Ordering, Behavior, Basic Husbandry, Identification, Handling, Breeding, Restraint Devices, Sexing, Transport, Anesthesia, Euthanasia, Rodent Surgery, Blood Collection, Administration of Fluid. Univ. Iowa. Michael Parker. - [Read Biomethodology of the Mouse]

Biomethodology of the Rabbit - http://research.uiowa.edu/animal/?get=rabbit

Category: Animal Research Techniques

Rabbit Biology, Sources Ordering, Behavior, Basic Husbandry, Identification, Handling, Breeding, Restraint Devices, Sexing, Transport, Anesthesia, Euthanasia, Rodent Surgery, Blood Collection, Administration of Fluid. Univ. Iowa. Michael Parker. - [Read Biomethodology of the Rabbit]

Biomethodology of the Rat - http://research.uiowa.edu/animal/?get=rat

Category: Animal Research Techniques

Rat Biology, Sources Ordering, Behavior, Basic Husbandry, Identification, Handling, Breeding, Restraint Devices, Sexing, Transport, Anesthesia, Euthanasia, Rodent Surgery, Blood Collection, Administration of Fluid. Univ. Iowa. Michael Parker. - [Read Biomethodology of the Rat]

Cartilage Staining of Xenopus tropicalis Protocol - http://tropicalis.berkeley.edu/home/gene_expression/cartilage-stain/alcian.html

Category: Xenopus Protocols

Protocol describes the use of a basic water-based dye to stain for acid mucosubstances and acetic mucins located on the cartilage of fixed embryos, permitting the examination of cartilage formation. - [Read Cartilage Staining of Xenopus tropicalis Protocol]

Cell Culture Protocols. Basic Topics in PDF - http://www.cf.ac.uk/phrmy/PCB/PageCellCultProtocols.html

Category: Cell Biology and Cell Culture

Cell Culture Protocols. Basic Topics in PDF. Gumbleton Lab PCB. - [Read Cell Culture Protocols. Basic Topics in PDF]

Confocal Microscopy: Speciman Preparation and Imaging - http://www.microscopyu.com/articles/confocal/confocalintropreparation.html

Category: Microscopy Protocols: Confocal Microscopy Protocols

Basic information on confocal microscopy, includes: Specimen Preparation and Imaging; Objective Lens Parameters and Optical Section Thickness; The Objective Lens; Probes for Confocal Imaging; Autofluorescence; Collecting Images; Troubleshooting; Image Processing and Publication; - [Read Confocal Microscopy: Speciman Preparation and Imaging]

DNA Isolation from Onion Cells - http://www.accessexcellence.org/AE/AEC/AEF/1994/dollard_onionDNA.html

Category: DNA Protocols: DNA Extraction Protocols

Allows students to easily isolate chromosomal DNA from onion cells using the same basic tools and methods that scientists use in the lab. A good introduction to using pipettes. Kate Dollard - [Read DNA Isolation from Onion Cells]

Flow Cytometry Analysis Protocol - http://www.cbrinstitute.org/labs/springer/protocols/qing_facs.html

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Sorting Protocols

Flow cytometry is a widely used method for characterizing and separating individual cells. This basic protocol focuses on: measure fluorescence intensity produced by fluorescent-labled antibodies and ligands that bind specific cell-associated molecules. Includes: Immunofluorescence Staining and Flow Cytometry Analysis. - [Read Flow Cytometry Analysis Protocol]

Flow Cytometry Analysis Protocol - http://www.cbrinstitute.org/labs/springer/protocols/qing_facs.html

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Flow cytometry is a widely used method for characterizing and separating individual cells. This basic protocol focuses on: measure fluorescence intensity produced by fluorescent-labled antibodies and ligands that bind specific cell-associated molecules. Includes: Immunofluorescence Staining; Flow Cytometry Analysis. - [Read Flow Cytometry Analysis Protocol]

In situ Hybridization - http://osiris.rutgers.edu/~smm/in_situ_hybridization.htm

Category: Cytogenetics Protocols: In Situ Hybridization Protocols

Describes the basic principles of in situ hybridization and advantages and disadvantages of different methodologies that can be used. Includes: Probe Selection; Probe Generation; Probe Labels; Fixation of Tissue; Hybridization and Washing; Control Procedures. - [Read In situ Hybridization]

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Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.