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Epstein-Barr Virus Transformation of Lymphoblasts Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4481

Category: Immunology

Protocol for Epstein-Barr Virus transformation of lymphoblasts. Protocol describes a method for the transformation of lymphoblasts by Epstein-Barr Virus (EBV). Cells may be isolated from whole blood or taken from cryopreserved, non-immortalized stocks. - [Read Epstein-Barr Virus Transformation of Lymphoblasts Protocol]

In Situ Hybridization for Epstein Barr Virus-Encoded RNA (EBER) Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/EBER-ISH4doublestain.html

Category: Immunohistochemistry Protocols: Antibody Staining Protocols

Protocol for in situ hybridization for Epstein Barr virus-encoded RNA (EBER). Includes: Glassware decontamination; Double staining for EBER and a second antibody . - [Read In Situ Hybridization for Epstein Barr Virus-Encoded RNA (EBER) Protocol]

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Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

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