autoclave Protocols

Search results for: autoclave

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Antigen Retrieval for immunohistochemistry - http://herpesvirus.tripod.com/research/protoc.htm#Antigen%20Retrieval%20for

Category: Immunology: Antigen Retrieval

Antigen Retrieval for immunohistochemistry. Microwave method, Autoclave method. slides and staining. Janos Luka. Eastern Virginia. - [Read Antigen Retrieval for immunohistochemistry]

Can you autoclave liquid Media? FAQ - http://invitrogen.custhelp.com/cgi-bin/invitrogen.cfg/php/enduser/std_adp.php?p_sid=HczfJTng&p_lva=1&p_faqid=120

Category: Cell Biology and Cell Culture: Cell Culture Growth Media

Most media cannot be autoclaved. With description. Invitrogen - [Read Can you autoclave liquid Media? FAQ]

Genetic Engineering with PCR Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3836

Category: PCR Protocols

Method describes how to modify the termini of PCR products by introducing restriction sites and other features. To reduce the chance of contamination with exogenous DNAs, prepare and use a special set of reagents and solutions for PCR only. Bake all glassware for 6 hours at 150°C and autoclave all plasticware. - [Read Genetic Engineering with PCR Protocol]

Genetic Engineering with PCR Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3836

Category: PCR Protocols: PCR Optimization

Laboratory Safety Program - http://www.uwm.edu/Dept/EHSRM/LAB/index.html

Category: General Research Protocols and Methods: Laboratory Safety

Laboratory Safety Program - Emergency Preparedness, Autoclave Safety, Biosafety, Carcinogens, Chemical Hygiene Plan, Glass Disposal, Fire Safety, Eyewash and Safety Shower Information, Fume Hood Procedures and Practices. University of Wisconsin - Milwaukee. - [Read Laboratory Safety Program]

Long PCR Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3841

Category: PCR Protocols: Standard PCR Protocol

Protocol can be used to amplify DNA up to 25 kb in length. To reduce the chance of contamination with exogenous DNAs, prepare and use a special set of reagents and solutions for PCR only. Bake all glassware for 6 hours at 150°C and autoclave all plasticware. - [Read Long PCR Protocol]

Articles (1-6 of 6)

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol. This protocol contains the steps for 3' end rapid amplification of mRNA by PCR. The first-strand cDNA is synthesized from total or poly(A+) RNA by priming from the poly-A tail of the mRNA using a oligo (dT) adaptor primer. The cDNA is then amplified via PCR using a gene-specific primer and an adaptor primer.

[1-6]