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Chromatin Assembly on Template DNA with Transcription Factors and Drosophila S-190 Chromatin Assembl - http://www.bio.com/protocolstools/protocol.jhtml%3Bjsessionid%3D3APITJJIWFIM1R3FQLMCFEWHUWBNSIV0?id=p9061

Category: Drosophila Protocols: Drosophila Genetics Protocols

Protocol described here produces chromatin with regularly spaced nucleosomes having physiological nucleosome repeat lengths; something that can be difficult to achieve with purified components. In addition, chromatin assembled with the S-190 Chromatin Assembly Extract contains the ATP-dependent chromatin remodeling factors necessary for efficient transcription. - [Read Chromatin Assembly on Template DNA with Transcription Factors and Drosophila S-190 Chromatin Assembl]

CSF Extract Prep for Xenopus Spindle Assembly Protocol - http://mitchison.med.harvard.edu/protocols/ext3.html

Category: Xenopus Protocols: Xenopus Egg Extracts Protocols

Protocol for CSF extract prep for Xenopus spindle assembly. - [Read CSF Extract Prep for Xenopus Spindle Assembly Protocol]

Cytostatic Factor (CSF) Extract Preparation for Spindle Assembly Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1422

Category: Cell Biology and Cell Culture: Cell Cycle Protocols: Mitosis Protocols

Cytostatic factor extract (CSF) preparation for spindle assembly protocol. Protocol includes tips such as: The quality of the eggs is essential for good CSF extracts. Always sacrifice quantity for quality when trying to make functional extracts. - [Read Cytostatic Factor (CSF) Extract Preparation for Spindle Assembly Protocol]

Cytostatic Factor (CSF) Extract Preparation for Xenopus Spindle Assembly Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1422

Category: Xenopus Protocols: Xenopus Egg Extracts Protocols

Protocol for cytostatic factor extract preparation for spindle assembly. The quality of the eggs is essential for good CSF extracts. Always sacrifice quantity for quality when trying to make functional extracts. Discard any batches of eggs that have "puff balls" or activated eggs that constitute more than 10% of the eggs. Use laid eggs and collect eggs at about 16 to 17 hours after priming with Progesterone (found in Pregnant Mare Serum Gonadotropin (PMSG)). - [Read Cytostatic Factor (CSF) Extract Preparation for Xenopus Spindle Assembly Protocol]

Drosophila Chromatin Assembly Protocol (S150 extract) - http://www.fhcrc.org/science/labs/tsukiyama/protocols/chromatin_assembly_S150_.pdf

Category: Drosophila Protocols: Drosophila Genetics Protocols

Protocol for Drosophila chromatin assembly (S150 extract). - [Read Drosophila Chromatin Assembly Protocol (S150 extract)]

M5 Velocity (rate zonal) gradients for purification and assembly analysis of viruses. - http://www.axis-shield.com/densityhome/optiprep/M05.pdf

Category: Cell Biology and Cell Culture

Purification protocols of the viruses: HIV-1, Lassa virus, oncornavirus and other retroviruses. Protocol uses an iodixanol gradient in a sedimentation velocity mode to purifyHIV-1 virions without affecting the infectivity of the virus. In rate-zonal iodixanol gradients the HIV-1 was effectively separated both from Vif and from the microvesicles. - [Read M5 Velocity (rate zonal) gradients for purification and assembly analysis of viruses.]

Microtubule Assembly Protocol - http://www.proweb.org/kinesin/Methods/MT_assembly.html

Category: Cell Biology and Cell Culture

Protocol on microtubule assembly using purified tubulin. - [Read Microtubule Assembly Protocol]

Nucleosomal Array Disruption Assay Protocol - http://www.bio.com/protocolstools/protocol.jhtml%3Bjsessionid%3D3APITJJIWFIM1R3FQLMCFEWHUWBNSIV0?id=p9058

Category: Drosophila Protocols: Drosophila Genetics Protocols

Protocol describes how to test whether a transcription factor disrupts the chromatin of a promoter of a gene of interest. First, chromatin is assembled in vitro on the gene of interest in the presence and absence of a transcriptional activator (see Protocol on Assembly of Chromatin with Drosophila S-190 Chromatin Assembly Extract and Transcriptional Activators). - [Read Nucleosomal Array Disruption Assay Protocol]

Preparation of Xenopus Sperm Nuclei Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p877

Category: Xenopus Protocols: Xenopus Egg Extracts Protocols

Protocol describes the preparation of nuclei from Xenopus sperm. These nuclei can be used for spindle assembly assays, microscopy, or DNA synthesis. - [Read Preparation of Xenopus Sperm Nuclei Protocol]

Protocol for Microtubule Assembly - http://www.bio.com/protocolstools/protocol.jhtml?id=p1600

Category: Cell Biology and Cell Culture

Purified Tubulin is polymerized and then stabilized with Taxol. The resulting Microtubules are then ready for use in motility assays. Protocol use solutions such as 50 mM Mg-GTP, 50 Î¼l 100 mM GTP, 40 Î¼M Taxol, Taxol Stock,and PM Buffer. - [Read Protocol for Microtubule Assembly]

Protocol Spindle Assembly In Vitro - http://www.bio.com/protocolstools/protocol.jhtml?id=p1051

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

This protocol describes an in vitro reaction to assay mitotic spindle assembly. The assay uses Cytostatic Factor extract made from Xenopus eggs, fluorescently-labelled tubulin, and prepared sperm nuclei. Spindle assembly is monitored by immunofluorescence microscopy. - [Read Protocol Spindle Assembly In Vitro]

Purification of Peptides from Solid-Phase Peptide Synthesis with RP-HPLC Protocol - http://cshprotocols.cshlp.org/cgi/content/abstract/2006/28/pdb.prot4548

Category: Chromatography Protocols: Liquid Chromatography Protocols

Protocol details the purification and analysis of many synthetic peptides of 2-65 amino acid residues. These peptides contain a number of ionizable or polar side chains, but do not contain secondary structural elements (such as ß-sheets) that favor supramolecular assembly. - [Read Purification of Peptides from Solid-Phase Peptide Synthesis with RP-HPLC Protocol]

Xenopus Egg CSF Extract Preparation Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1049

Category: Xenopus Protocols: Xenopus Egg Extracts Protocols

Protocol describes the preparation of Cytostatic Factor (CSF) from frog eggs. CSF can be used in in vitro spindle assembly assays. Includes: Frog injections; Preparation Before Starting the Extract Preparation; Extract Preparation. - [Read Xenopus Egg CSF Extract Preparation Protocol]

Articles (1-1 of 1)

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.