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ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

Search Results Protocol Links Sort by: Hits | Alphabetical

Micronucleus assay Human Lymphocytes - http://ehs.sph.berkeley.edu/holland/protocolLibrary/Cytogenetics_MN_Lymphocytes.html

Category: Cytogenetics Protocols

MN in Human Lymphocytes (method and protocol description) Nina T. Holland, Division of Environmental Health Sciences, Berkeley - [Read Micronucleus assay Human Lymphocytes]

Microsome High Speed Pellet and Cytosol Preparation for Endoplasmic Reticulum to Golgi Transport - http://www.bio.com/protocolstools/protocol.jhtml?id=p1841

Category: Yeast Protocols

Protocol for microsome high speed pellet and cytosol preparation for endoplasmic reticulum to golgi transport assay in Yeast. - [Read Microsome High Speed Pellet and Cytosol Preparation for Endoplasmic Reticulum to Golgi Transport]

Microsome High Speed Pellet and Cytosol Preparation for Endoplasmic Reticulum to Golgi Transport Ass - http://www.bio.com/protocolstools/protocol.jhtml?id=p1841

Category: Cell Organelle Protocols: Microsome Protocols

Protcol relating to microsome high speed pellet and cytosol preparation for endoplasmic reticulum to golgi transport assay in yeast. Includes spheroplast formation. - [Read Microsome High Speed Pellet and Cytosol Preparation for Endoplasmic Reticulum to Golgi Transport Ass]

Microtubule/ organelle Motility Assays - http://www.bio.unc.edu/faculty/salmon/lab/protocolsmtorganellemotilityassays.html

Category: Cell Biology and Cell Culture

Microtuble/ organelled motility assay using Golgi or ER membranes, 45 uM tubulin, rat liver cytosol, and 20x energy regeneration system. - [Read Microtubule/ organelle Motility Assays]

Minichromosome Microtubule BInding Assay Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/MICROTUBULE/mmb.html

Category: Cytoskeleton Protocols: Microtubules Protocols

Protocol for minichromosome microtubule binding assay. - [Read Minichromosome Microtubule BInding Assay Protocol]

Molecular and Genetic Toxicology Studies for In Vivo Cytogenetics - http://www.sri.com/biosciences/bdd/toxic/protocol.html#8

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols

Molecular and genetic toxicology studies for in vivo cytogenetics. Includes: G501 Chromosomal Aberrations in Rodent Bone Marrow Cells, G502 Single-Exposure Bone Marrow Cytotoxicity Assay. - [Read Molecular and Genetic Toxicology Studies for In Vivo Cytogenetics]

Molecular and Genetic Toxicology Studies on Gene Mutation in Mammalian Cells In Vitro - http://www.sri.com/biosciences/bdd/toxic/protocol.html#5

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols

Molecular and genetic toxicology studies on gene mutation in mammalian cells In vitro. Includes: G201 Mouse Lymphoma L5178Y/tk+/Cell Gene Mutation Assay; G201-R; G202 Mouse Lymphoma L5178Y/tk+/Cell Gene Mutation Screening Assay; G203 Chinese Hamster Ovary or V79 Cell Mutation Assay at the hprt Locus; G203R. - [Read Molecular and Genetic Toxicology Studies on Gene Mutation in Mammalian Cells In Vitro]

Molecular and Genetic Toxicology Studies on Microbial Mutagenesis - http://www.sri.com/biosciences/bdd/toxic/protocol.html#4

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols

Molecular and genetic toxicology studies on microbial mutagenesis. Includes: G101 Salmonella/Microsome Plate Incorporation Assay (Ames Test), G101-R, G102 Salmonella/Microsome Preincubation Assay (Ames Test), G102-R, G103 Salmonella/E. Coli Reverse Mutation Assay (using four Salmonella and one E. coli strains), G104 Salmonella/E. Coli Reverse Mutation Assay (using four Salmonella and two E. coli strains), G105, G106. - [Read Molecular and Genetic Toxicology Studies on Microbial Mutagenesis]

Monoamine Oxidase Assay Protocol - http://www.promega.com/tbs/tb345/tb345.pdf

Category: Pharmacology and Toxicology Protocols: Enzyme Assays Protocols

Protocol for monoamine oxidase assay. - [Read Monoamine Oxidase Assay Protocol]

MONOCYTE AND NEUTROPHIL ISOLATION - http://aactg.s-3.com/pub/download/imm/phagocyt.pdf

Category: Immunology: Chemotaxis

MONOCYTE AND NEUTROPHIL ISOLATION. The protocol describes the method used for isolation of monocytes and neutrophils used in both the chemotaxis assay and microbicidal assays. Pediatric AIDS Clinical Trials Group - [Read MONOCYTE AND NEUTROPHIL ISOLATION]

Monster Green® Fluorescent Protein Assay - http://www.promega.com/catalog/country_select.asp?/tbs/tb320/tb320.html&ckt=2

Category: Reporter Gene Assays: GFP Assay Protocols

Green fluorescent protein is commonly used to monitor gene expression and protein trafficking within intact cells. The Monster Green® Fluorescent Protein is encoded by an improved synthetic version of the green fluorescent protein gene originally cloned from Montastrea cavernosa (Great Star Coral). - [Read Monster Green® Fluorescent Protein Assay]

Mouse ELISPOT-Assay Protocol - http://www.aelvis.net/pdf/ELISPOTProtocolMice.pdf

Category: ELISA Protocols: ELISpot Protocols

Protocol for mouse ELISPOT assay. - [Read Mouse ELISPOT-Assay Protocol]

MTT Assay for Detection of Cellular Proliferation - http://www.ebioscience.com/ebioscience/appls/AC145.htm#mouse

Category: Immunology: T Cell Protocols

Mouse Protocol: Stimulation of mouse peripheral T cells with plate-bound 145-2C11 monoclonal antibody; MTT assay for detection of cellular proliferation. - [Read MTT Assay for Detection of Cellular Proliferation]

MTT Cell Proliferation Assay Protocol - http://www.atcc.org/pdf/30-1010k.pdf

Category: Cell Biology and Cell Culture: Cell Viability Protocols

The MTT Cell Proliferation Assay measures the cell proliferation rate and conversely, when metabolic events lead to apoptosis or necrosis, the reduction in cell viability. The number of assay steps has been minimized as much as possible to expedite sample processing. The MTT Reagent yields low background absorbance values in the absence of cells. Includes: Determining optimal cell counts, performing an assay, data interpretations and troubleshooting. - [Read MTT Cell Proliferation Assay Protocol]

Needle Assay for Chemotaxis - http://www.hopkinsmedicine.org/cellbio/devreotes/needle.htm

Category: Immunology: Chemotaxis

Needle Assay for Chemotaxis. Devreotes Lab, John Hopkins Medical Centre. - [Read Needle Assay for Chemotaxis]

Neuron Culture Solutions - http://www.ehlerslab.org/protocols.html

Category: Neuroscience: Neuron Cell Culture Protocols

Neuron Cell Culture Solutions, Neuron Culturing, Preparation of Brain Membrane Fractions, Biotinylation Assay of Receptor Endocytosis, PSD Prep. Ehlers lab Duke Univ. - [Read Neuron Culture Solutions]

Neuron Fibroblast Coculture Assay to Demonstrate Presynaptic Differentiation - http://www.natureprotocols.com/2006/09/26/neuronfibroblast_coculture_ass.php

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols

Neuron-fibroblast coculture assay to demonstrate presynaptic differentiation. Includes: Preparation of HEK293T cells, Coculture of HEK293T cells and neurons, Immunostaining, Vesicle turnover experiment, Image acquisition and quantitation. - [Read Neuron Fibroblast Coculture Assay to Demonstrate Presynaptic Differentiation]

Neutral Red Cytotoxicity Assay Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/64.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cytotoxicity Assays in Cell Culture Protocols

The cytotoxic effect of chemicals upon mammalian cells, such as BALB/c 3T3 and HepG2, in culture is measured by highest tolerated dose (HTD), cell viability (Neutral Red) and total cell protein (coomassie blue). - [Read Neutral Red Cytotoxicity Assay Protocol]

Nucleosomal Array Disruption Assay Protocol - http://www.bio.com/protocolstools/protocol.jhtml%3Bjsessionid%3D3APITJJIWFIM1R3FQLMCFEWHUWBNSIV0?id=p9058

Category: Drosophila Protocols: Drosophila Genetics Protocols

Protocol describes how to test whether a transcription factor disrupts the chromatin of a promoter of a gene of interest. First, chromatin is assembled in vitro on the gene of interest in the presence and absence of a transcriptional activator (see Protocol on Assembly of Chromatin with Drosophila S-190 Chromatin Assembly Extract and Transcriptional Activators). - [Read Nucleosomal Array Disruption Assay Protocol]

Nucleosome Spacing Assay Protocol - http://www.fhcrc.org/science/labs/tsukiyama/protocols/nucleosome_spacing_assay.pdf

Category: Molecular Biology Protocols

Protocol for nucleosome spacing assay. - [Read Nucleosome Spacing Assay Protocol]

Pediatric Flow Cytometric Enumeration of Apoptotic Cells PDF. - http://pactg.s-3.com/pub/download/imm/PedsApo.pdf

Category: Cell Biology and Cell Culture: Apoptosis Protocols: TUNEL assay apoptosis

Pediatric Flow Cytometric Enumeration of Apoptotic Cells Using the Tunel Assay. PDF. Nichols et al. PedsAPO - [Read Pediatric Flow Cytometric Enumeration of Apoptotic Cells PDF.]

Phagocytosis Assay - http://www.hopkinsmedicine.org/cellbio/devreotes/phagocytosis.htm

Category: Protein Protocols: Protein Trafficking

Protocol and information on phagocytosis assay. - [Read Phagocytosis Assay]

Phagocytosis Assay Protocol - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./protocol5.html~main

Category: Fungi Protocols

Protocol for phagocytosis assay. Protocol describes how to assess the efficiency of phagocytic cells to engulf conidia of a given fungus. - [Read Phagocytosis Assay Protocol]

Pheromone Halo Assay Protocol - http://www.med.unc.edu/%7Ehdohlman/haloassay.html

Category: Signalling Signal Transduction Protocols

Pheromone Halo Assay. Dohlman Lab Protocol. An assay which measures the responsiveness and effects of cells to a factor pheromone. - [Read Pheromone Halo Assay Protocol]

Pheromone Halo Assay Protocol - http://www.med.unc.edu/~hdohlman/haloassay.html

Category: Yeast Protocols

Protocol for pheromone halo assay. - [Read Pheromone Halo Assay Protocol]

Articles (1-5 of 5)

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Coupling Cysteine Containing Peptides to Carrier Protein for Immunization and Polyclonal Antibody

This protocol is used in the production of polyclonal antibodies that recognize a peptide sequence of interest.

Histone H1 Kinase Activity Assay

Histone H1 Kinase Activity Assay Protocol. This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography.

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