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ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

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In Vitro Micromass Teratogen Assay - http://embryo.ib.amwaw.edu.pl/invittox/prot/114.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cell Specific Cytotoxicity Assays Protocols

The effect of a test compound, in the presence and absence of S-9 mix, on the differentiation and growth of rat limb bud and CNS cells in vitro indicates whether it is potentially a teratogen in vivo. - [Read In Vitro Micromass Teratogen Assay]

In Vitro Transcription Assay (Run-off Assay) using Permeabilized Cells - http://www.bio.com/protocolstools/protocol.jhtml?id=p1971

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

With this protocol, transcripts that were initiated from specific genes by RNA polymerases prior to permeabilization can be measured. Instead of a nuclear extract, permeabilized cells are used. Includes information on: Permeabilization of Cells; In vitro Transcription Reaction (Run-off); Isolation of RNA; Preparation of Slot Blot Membrane for Hybridization; Hybridization of Nitrocellulose Membrane; TCA Precipitation to Determine Incorporation of [32P] GTP into Nucleic Acid - [Read In Vitro Transcription Assay (Run-off Assay) using Permeabilized Cells]

In Vitro Transcription With Yeast Nuclear Extract - http://www.fhcrc.org/science/labs/hahn/methods/biochem_meth/polii_trans.html

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol for in vitro transcription with yeast nuclear extract. Includes recipes for: 5x Acetate transcription buffer; Creatine phospho kinase; Phospho creatine; Stop mix; HA + 0.1 M potassium acetate; 5x glutamate transcription buffer (5 ml). Includes also protocol for Primer Extension Assay. - [Read In Vitro Transcription With Yeast Nuclear Extract]

In vitro Translation Assays for CFTR - http://pen2.igc.gulbenkian.pt/cftr/vr/c/ostedgaard_in_vitro_translation_assays_for_cftr.pdf

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol outlines the general procedure and requirements for in vitro translation of CFTR and outlines some assays using in vitro translated product. Core glycosylation of CFTR occurs in the ER. An assay for this processing step requires the addition of microsomal membranes to the basic in vitro translation mixture. This protocol takes this into account. - [Read In vitro Translation Assays for CFTR]

In Vivo Cytokine Capture Assay (IVCCA) Protocol - http://www.ebioscience.com/ebioscience/appls/IVCCA.htm

Category: Immunology: Immunoassays

Protocol for in vivo cytokine capture assay (IVCCA). - [Read In Vivo Cytokine Capture Assay (IVCCA) Protocol]

In-Gel Kinase Assay - http://www.bio.com/protocolstools/protocol.jhtml?id=p911

Category: Signalling Signal Transduction Protocols

Protocol describes how to assay for kinase activity within a polyacrylamide gel, rather than in solution. The advantages to an in-gel assay are that an apparent molecular weight can be assigned to the kinase activity and that multiple kinase activities can be distinguished. Includes protocol hints. - [Read In-Gel Kinase Assay]

In-Vitro Kinase Assay for Jnk-1/IRS Proteins - http://www.whitelabs.org/Lab%20Protocols/kinase%20and%20phosphatase%20assays/In_vitro%20kinase%20assay%20for%20JNK-1%20IRS%20proteins.htm

Category: Signalling Signal Transduction Protocols: MAPK Protocols

Protocol describing an in vitro assay on the detection of Kinase activity for Jnk-1/IRS proteins. - [Read In-Vitro Kinase Assay for Jnk-1/IRS Proteins]

JEG3 Transplantation Assay Protocol - http://www.natureprotocols.com/2006/08/07/jeg3_transplantation_assay.php

Category: Developmental Biology: Utilizing Model Organisms Protocols

Protocol for JEG3 transplantation assay. - [Read JEG3 Transplantation Assay Protocol]

JNK Kinase Assay - http://www.whitelabs.org/Lab%20Protocols/kinase%20and%20phosphatase%20assays/JNK%20Kinase%20Assay%20LRui.htm

Category: Signalling Signal Transduction Protocols: MAPK Protocols

Protocol describing an JNK Kinase Assay. Protocol includes information on reaction buffer. - [Read JNK Kinase Assay]

Kinase Assay Using In Vitro Translated Xenopus Mos Kinase - http://www.bio.com/protocolstools/protocol.jhtml?id=p912

Category: Signalling Signal Transduction Protocols

Protocol describes how tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay. Protocol includes: Translation of Xenopus Mos Kinase; Antibody to Antigen Binding; Protein A Sepharose to Antibody Binding; Kinase Reactions on Immunoprecipitated Material; Polyacrylamide Gel Analysis of Immunoprecipitates. Includes protocol hints. - [Read Kinase Assay Using In Vitro Translated Xenopus Mos Kinase]

Kinase Assays - http://www.natureprotocols.com/2007/01/29/kinase_assays.php

Category: Signalling Signal Transduction Protocols: MAPK Protocols

Nature protocol entailing a kinase assay. - [Read Kinase Assays]

Liquid Beta-galactosidase Reporter Gene Assay Protocol - http://www.med.unc.edu/~hdohlman/bgal.html

Category: Yeast Protocols: Yeast Genetics Protocols: B-Galactosidase Assay Protocols

Protocol for liquid beta-galactosidase reporter gene assay. - [Read Liquid Beta-galactosidase Reporter Gene Assay Protocol]

List of Protein Assays - http://www.ruf.rice.edu/~bioslabs/methods/protein/protein.html

Category: Protein Protocols: Protein Quantitation Concentration Protocols

Find a list of assays for the determination of protein concentration in a solution. This list includes the sensitivity range, volume/amount of sample needed, subjective comments on accuracy and convenience, and major interfering agents. Procedural details, equipment requirements, and references are outlined in the individual assay documents. - [Read List of Protein Assays]

Live Dead Assay for Cell Viability Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000023.pdf?pid=PP00000023

Category: Immunology: B Cell Protocols

This assay is used to measure cell viability. It is a two-color fluorescence assay that simultaneously determines: Live cell number and Dead cell number. - [Read Live Dead Assay for Cell Viability Protocol]

Live/Dead Assay for Cell Viability Protoco - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000023.pdf?pid=PP00000023

Category: Cell Biology and Cell Culture: Cell Viability Protocols

Assay measures cell viability. It is a two-color fluorescence assay that simultaneously determines Live cell number and Dead cell number. This protocol is designed for use with the GEMINI XS Microplate Spectrofluorometer, a multi-well plate scanner with dual excitation/emission capabilities, but the assay is also adaptable for flow cytometry and fluorescence microscopy. Includes: Cell Culture; Preparation for the Assay; Live/Dead Assay; Reading the Plate; Data Analysis; Alternative protocol. - [Read Live/Dead Assay for Cell Viability Protoco]

Lowry â€“ Protein Determination - http://wolfson.huji.ac.il/purification/Protocols/loury.html

Category: Protein Protocols: Lowry Protein Assay

Lowry â€“ Protein Determination. Quantitation of total protein using the lowry protein assay. Dr. Mario Lebendiker. The Wolfson Centre for Applied Structural Biology, The Hebrew University of Jerusalem. Method materials and notes for the Lowry assay. - [Read Lowry â€“ Protein Determination]

Lowry Protein Assay - http://www.animal.ufl.edu/hansen/protocols/LOWRY.htm

Category: Protein Protocols: Lowry Protein Assay

Lowry Protein Assay. The Lowry procedure is one of the most venerable and widely-used protein assays, being first described in 1951 [Lowry et al., J. Biol. Chem. 193: 265-275 (1951)]. Under alkaline conditions, copper complexes with protein. When folin phenol reagent (phospho-molybdic-phosphotungstic reagent) is added, the Folin-phenol reagent binds to the protein. Bound reagent is slowly reduced and changes color from yellow to blue. P.J. Hansen, Dept. of Animal Sciences, University of Florida. - [Read Lowry Protein Assay]

Lowry Protein Assay Protocol - http://ylwang.umassmed.edu/protocol/pa/lowry.htm

Category: Protein Protocols: Lowry Protein Assay

Lowry Protein Assay Protocol. Yu-li Wang Lab, Univ. Massachusetts Medical School. Detailed protocol including materials. - [Read Lowry Protein Assay Protocol]

Luciferase Assay 24 Well Plate (Promega System) - http://www.biochem.northwestern.edu/ibis/morimoto/research/Protocols/II.%20Eukaryotes/F.%20Luciferase%20Assays/2a.single%20luciferase.pdf

Category: Reporter Gene Assays: Luciferase Assay Protocols

Luciferase assay using a 24 well plate. Includes: Cell Lysis; Reagent Preparation; Luminescence measurement; Wash injector; Protein quantitation; Data Analysis. - [Read Luciferase Assay 24 Well Plate (Promega System)]

Luciferase Assay Protocol for HEK COS cells - http://www.ucc.ie/ucc/depts/physio/MolPhysiol/MolPhys/Protocols/pdfs/012.pdf

Category: Luciferase and Dual Luciferase Assays

Luciferase Assay Protocol for HEK COS cells. University College Cork UCC. Dept. Physiology. - [Read Luciferase Assay Protocol for HEK COS cells]

Luciferse Assay Protocol for 12 Well Plates. Word Doc. - http://www.med.umich.edu/intmed/endocrinology/hammerlab/Protocols/Luciferase%20Assay%20Protocol.doc

Category: Luciferase and Dual Luciferase Assays

Luciferse Assay Protocol for 12 Well Plates. Word Doc. Hammer Lab. - [Read Luciferse Assay Protocol for 12 Well Plates. Word Doc.]

Lung Cell Assay Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/48.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cell Specific Cytotoxicity Assays Protocols

Potential embryotoxicity is assessed by monitoring the effect of the test compound on total protein synthesis, and DNA synthesis in cultured human foetal lung fibroblasts. Rat lung epithelial cells can be used to determine cytotoxicity of select compounds because of their ability to metabolise xenobiotics. - [Read Lung Cell Assay Protocol]

Mammalian Toxicology Acute Toxicity Studies - http://www.sri.com/biosciences/bdd/toxic/protocol.html#1

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols

Mammalian toxicology for acute toxicity studies. Includes: M101 Acute Limit Test (Rodents), M102 Acute Toxicity Study (Rats or Mice), M120 Dose Escalation Study (Dog), M103 Acute Dermal Limit Test, M104 Acute Dermal (LD50), Study M105 Primary Dermal Irritation, M106 Primary Eye Irritation Study, M106-A, M110 Local Lymph Node Assay in Mouse, M115 Rabbit Vaginal Irritation Assay. - [Read Mammalian Toxicology Acute Toxicity Studies]

Measurement of Intracellular Ions by Flow Cytometry Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E661C4C9AC9935B31A1C2087E2276A3&objectid=6674EF32EE59A37C48B4A267D733CAE2

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols

Describes flow cytometric protocols using the dyes Indo-1 AM, Fluo-3, and Fura Red AM to measure intracellular calcium concentration. Support protocols detail the use of calcium buffers to calibrate a flow cytometric calcium assay, and methods to facilitate dye loading; an alternate protocol describes the use of a spectrofluorimeter to measure intracellular calcium for those investigators without access to a flow cytometer. - [Read Measurement of Intracellular Ions by Flow Cytometry Protocol]

Micro-Glutathione Assay Protocol - http://www.animal.ufl.edu/hansen/protocols/gsh.htm

Category: Developmental Biology: Embryology Protocols

Protocol for micro-glutathione assay. - [Read Micro-Glutathione Assay Protocol]

Articles (1-5 of 5)

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Coupling Cysteine Containing Peptides to Carrier Protein for Immunization and Polyclonal Antibody

This protocol is used in the production of polyclonal antibodies that recognize a peptide sequence of interest.

Histone H1 Kinase Activity Assay

Histone H1 Kinase Activity Assay Protocol. This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography.

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