User Panel

My Panel

Bookmark Science Articles

assay Protocols

Search results for: assay

Protocols » Search Results

Featured Search Results Links

ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

Search Results Protocol Links Sort by: Hits | Alphabetical

DNA-Methyltransferase Assay - Anderson Centre - http://www.mdanderson.org/departments/methylation/display.cfm?id=9B5B6082-C53A-4A86-BEE3EE149A52251E&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: Epigenetics and Methylation Protocols

M.D. Anderson Cancer Centre Texas - [Read DNA-Methyltransferase Assay - Anderson Centre]

DNA-Methyltransferase Assay Protocol - http://www.mdanderson.org/departments/methylation/display.cfm?id=9B5B6082-C53A-4A86-BEE3EE149A52251E&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: Nucleic Acid Modification Protocols: DNA Methylation Protocols

Protocol for DNA-methyltransferase assay. Includes: Homogenize Cells/Tissues; Quantitate Protein Concentration; Dilute Samples; The Assay Proper; Troubleshooting. - [Read DNA-Methyltransferase Assay Protocol]

DNaseI Footprinting Assay - http://www.fhcrc.org/science/labs/breeden/Methods/DNaseI-footprint.html

Category: DNA Protocols: EMSA DNA-Protein Protocols

DNaseI Footprinting Assay. Works well high affinity dna binding proteins. Breeden Lab. - [Read DNaseI Footprinting Assay]

DnaseI Footprinting Assay Protocol - http://ruummc.med.uu.nl/subsite/RepHTML/Protocols/DNasefootprinting.html

Category: DNA Protein Interactions Protocols: Dnase Footprinting Assay Protocols

Protocol for DnaseI footprinting assay. - [Read DnaseI Footprinting Assay Protocol]

Dual Luciferase Assay System. Promega. PDF - http://www.promega.com/tbs/tm040/tm040.pdf

Category: Luciferase and Dual Luciferase Assays

Dual Luciferase Assay System. Promega. PDF - [Read Dual Luciferase Assay System. Promega. PDF]

Dynamic Flow Assay for Cell Adhesion in a Parallel Plate Flow Chamber - http://www.glycotech.com/protocols/proto7.html

Category: Cell Biology and Cell Culture

Flow assays offer visualization of cell adhesion under wall shear stress. Visualization of the different events of cell adhesion can be quantified by selective image acquisition and subsequent image processing. Flow assays are suited for adhesive events which occur very rapidly in a time scale shorter than that of most static adhesion assays. Also, events subsequent to the initial events can be studied such as cell stabilization and spreading giving some insight into the kinetics of cell-cell. - [Read Dynamic Flow Assay for Cell Adhesion in a Parallel Plate Flow Chamber]

Dynamic Flow Assay in a Parallel Plate Flow Chamber - http://www.glycotech.com/protocols/proto7.html

Category: Cell Biology and Cell Culture: Cell Adhesion Protocols

Flow assays allow visualization of cell adhesion under well-defined wall shear stress. Visualization of the events of cell adhesion are quantified by selective image acquisition and image processing. Events subsequent to the initial events can be studied such as cell stabilization and spreading. John T. Patton~GlycoTech Corporation, Rockville, Maryland - [Read Dynamic Flow Assay in a Parallel Plate Flow Chamber]

Electro Mobility Shift Assay Protocol - http://ruummc.med.uu.nl/subsite/RepHTML/Protocols/EMSARdJ.html

Category: DNA Protein Interactions Protocols: Electrophoretic Mobility Shift Assays EMSA Protocols

Protocol electro mobility shift assay (EMSA) optimized for pTP. - [Read Electro Mobility Shift Assay Protocol]

Electrophoretic Mobility Shift Assay (EMSA) for the detection of nuclear NF-kB - http://www.celldeath.de/apometh/emsa.html

Category: DNA Protocols: EMSA DNA-Protein Protocols

Electrophoretic Mobility Shift Assay (EMSA) for the detection of nuclear NF-kB. CellDeath.de - [Read Electrophoretic Mobility Shift Assay (EMSA) for the detection of nuclear NF-kB]

ELISA (Enzyme-Linked ImmunoadSorbent Assay) - http://www.nottingham.ac.uk/~mbzspd/methods/ELISA.html

Category: ELISA Protocols

ELISA (Enzyme-Linked ImmunoadSorbent Assay) Method and technique. Jill Fergusson, Department of Biochemistry, University of Nottingham, - [Read ELISA (Enzyme-Linked ImmunoadSorbent Assay)]

ELISA for Antibody Isotype Determination Protocol - http://www.natureprotocols.com/2007/01/22/immunizations_and_enzymelinked.php

Category: ELISA Protocols

Protocol for immunizations and enzyme-linked immunosorbent assay (ELISA) for antibody isotype determination. - [Read ELISA for Antibody Isotype Determination Protocol]

ELISA for detection of platelet-associated Ig (PAIg) - http://www.icp.ucl.ac.be/mexp/vipg_plelisa.html

Category: ELISA Protocols

Qualitative ELISA (Enzyme-Linked Immunosorbent Assay) used for screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg). Andrei Musaji Viral immunity and pathogenesis group, Universite Catholique de Louvain. - [Read ELISA for detection of platelet-associated Ig (PAIg)]

ELISA Inhibition Assay - http://wheat.pw.usda.gov/~lazo/methods/goldberg/elisa.html

Category: ELISA Protocols

Protocol and method for ELISA Inhibition Assay. Michael Coyne. Goldberg Lab - [Read ELISA Inhibition Assay]

ELISPOT ASSAY Protocol PDF - http://www.usuhs.mil/mic/Gause_lab/ELISPOT_Protocol.PDF

Category: ELISA Protocols: ELISpot Protocols

ELISPOT ASSAY Protocol PDF. William C. Gause, Uniformed Services Univ. - [Read ELISPOT ASSAY Protocol PDF]

EMSA protocol (Word Doc) - http://www.med.umich.edu/intmed/endocrinology/hammerlab/Protocols/EMSA%20Protocol.doc

Category: DNA Protocols: EMSA DNA-Protein Protocols

Short EMSA protcol starting with gel running and supershift assay. Hammer Lab. - [Read EMSA protocol (Word Doc)]

Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF Protocol - http://crutcherlab.med.uc.edu/ELI.htm

Category: Immunology: Immunoassays

Protocol for ELISA assay for NGF. Includes: ABSORPTION OF THE POLYCLONAL AND PREIMMUNE SERUM; BLOCKING; SAMPLE PREPARATION; PREPARATION OF NGF STANDARDS; PROTEIN RECOVERY; DESIGNING THE PLATE; APPLYING THE STANDARDS AND SAMPLES; APPLYING THE MONOCLONAL; APPLYING SECONDARY ANTIBODY; APPLYING STREPTAVIDIN; CHROMAGEN DEVELOPMENT; READING THE PLATE. - [Read Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF Protocol]

Epitope Mapping by Competition Assay Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4277

Category: Antibody Protocols: Epitope Mapping Antibody Protocols

The simplest way to determine whether two monoclonal antibodies bind to distinct sites on a protein antigen is to carry out a competition assay. The assay can be used with antibodies that bind both conformational and linear epitopes, and it is most useful in the analysis of monoclonal antibody specificity because polyclonal sera typically recognize multiple different epitopes. - [Read Epitope Mapping by Competition Assay Protocol]

Erythrophagocytosis Assay - http://www.icp.ucl.ac.be/mexp/vipg_eph.html

Category: Immunology: Macrophage Monocyte Protocols

Erythrophagocytosis Assay. The erythrophagocytosis assay is performed to compare the phagocytic rate with and without anti-eythrocyte antibody in either macrophages from control animals or virus-infected counterparts. Andrei Musaji Viral Immunity and Pathogenesis Group - [Read Erythrophagocytosis Assay]

Exonuclease Assay Protocol - http://ruummc.med.uu.nl/subsite/RepHTML/Protocols/ExonucleaseAssay.html

Category: Molecular Biology Protocols

Protocol for exonuclease assay. - [Read Exonuclease Assay Protocol]

FAK Autophosphorylation Assay - http://www.bio.com/protocolstools/protocol.jhtml?id=p1984

Category: Signalling Signal Transduction Protocols

This kinase assay is meant to determine whether an agonist or event can influence the autophosphorylation of FAK. The addition of 1 Î¼l of polyGT to the kinase reaction mix will determine the activity of the enzyme against a substrate. Includes information on: Harvest, Immunoprecipitation, Kinase Reaction and Antibody Detection of FAK. - [Read FAK Autophosphorylation Assay]

Fam Caspase 8 and 9 Binding Assay for Embryos Protocol - http://www.animal.ufl.edu/hansen/Protocols/caspase_8_and_9_binding_assay.htm

Category: Developmental Biology: Embryology Protocols

The FAM caspase binding assay kits from ATCC Corporation can be used to determine amounts of active caspases in cells. The FAM-labeled caspase inhibitor can freely diffuse into the cell. Active caspase irreversibly binds the inhibitor. Upon washing the cells, the amount of fluorescence is proportional to the amount of active caspase in the cell. FAM-LETD-fmk (catalog no. 30-1306) is used to detect caspase 8 and FAM-LEHD-fmk (catalog no. 30-1308) is used for caspase 9. - [Read Fam Caspase 8 and 9 Binding Assay for Embryos Protocol]

Fatty Acid Oxidation Assay Protocol - http://www.natureprotocols.com/2006/12/15/fatty_acid_oxidation_assay.php

Category: Lipid Protocols

Protocol for fatty acid oxidation assay. Includes: Differentiation of C2C12 cells; Preparation; Oleic acid oxidation. - [Read Fatty Acid Oxidation Assay Protocol]

Fluorometric Quantitation of DNA Using Hoechst 33258 Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4458

Category: Nucleic Acid Detection Protocols

Protocol describes the quantitation of DNA using Hoechst 33258, a fluorescent dye that binds to double-stranded DNA. Fluorometry is simple and more sensitive than spectrophotometry, and allows the detection of nanogram quantities of DNA. The assay can only be used to measure the concentration of DNAs whose sizes exceed ~1 kb, as Hoechst 33258 binds poorly to smaller DNA fragments. - [Read Fluorometric Quantitation of DNA Using Hoechst 33258 Protocol]

FP-TDI Method for SNP Detection - http://las.perkinelmer.com/content/snps/fp-tdi.asp

Category: DNA Protocols: SNP Protocols

FP-TDI Method for SNP Detection. The FP-TDI protocol was originally reported by Drs. Chen, Levine, and Kwok at Washington University in 19991,2. FP-TDI stands for template directed dye terminator incorporation assay with detection by fluorescence polarization. It is a single base primer extension assay couple with homogeneous FP detection. Perkin Elmer - [Read FP-TDI Method for SNP Detection]

Gel Assay to Determine DNA Content of Phage Lysates Protocol - http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda3.html

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

A crude lysate gel assay can be performed to roughly quantitate the DNA in lysates. This is often a valuable time saving step to determine if the phage yield is sufficient to warrant continuing the procedure. - [Read Gel Assay to Determine DNA Content of Phage Lysates Protocol]

Articles (1-5 of 5)

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Coupling Cysteine Containing Peptides to Carrier Protein for Immunization and Polyclonal Antibody

This protocol is used in the production of polyclonal antibodies that recognize a peptide sequence of interest.

Histone H1 Kinase Activity Assay

Histone H1 Kinase Activity Assay Protocol. This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography.

[1-5]

Items 126-150 out of 277 displayed.