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ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

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Assay of Intracellular Free Calcium in Suspended B Cells PDF - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi?pid=PP00000011

Category: Signalling Signal Transduction Protocols: Calcium Measurement Protocols

Protocol for a method which assesses concentrations of free cytoplasmic calcium (Ca2+) in mouse splenic B cells in the absence and presence of ligands for cell surface receptors. Signalling Gateway - [Read Assay of Intracellular Free Calcium in Suspended B Cells PDF]

Assay of Intracellular Free Calcium in Suspended B Cells Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000011.pdf?pid=PP00000011

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols

This protocol describes a method to assess concentrations of free cytoplasmic calcium (Ca2+) in mouse splenic B cells in the absence and presence of ligands for cell surface receptors. This objective is accomplished with the Ca2+-sensitive fluorescent dye, Fluo-3, which permeates cells as an ester and is hydrolyzed in the cell to its Ca2+- sensitive acidic form. - [Read Assay of Intracellular Free Calcium in Suspended B Cells Protocol]

Assay of Intracellular Free Calcium in Suspended B Cells Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000011.pdf?pid=PP00000011

Category: Immunology: B Cell Protocols

Assay of β-Galactosidase in Yeast Protocol - http://www.oardc.ohio-state.edu/stockingerlab/Protocols/BetaGalAssays.pdf

Category: Reporter Gene Assays: B-Galactosidase Assay Protocols

There are two basic methods for the in vitro assay of B-galactosidase activity from yeast. They differ mainly in the method of preparing the material for assay. Both methods are described with accompanying protocols. Method I: Assay of Crude Extracts includes: Yeast Cell Growth; Yeast Cell Harvest; B-gal assays; Bradford Assays. Method II: Permeabilized cell assay. - [Read Assay of β-Galactosidase in Yeast Protocol]

Assay of β-Galactosidase in Yeast Protocol - http://www.oardc.ohio-state.edu/stockingerlab/Protocols/BetaGalAssays.pdf

Category: Yeast Protocols: Yeast Genetics Protocols: B-Galactosidase Assay Protocols

Protocol for assay of β-Galactosidase in Yeast. Includes: Assay of Crude Extracts; Yeast Cell Growth; Yeast Cell Harvest; β-gal assays; Bradford Assays; Permeabilized cell assay. - [Read Assay of β-Galactosidase in Yeast Protocol]

Assay:Single Round of In Vitro Transcription from Assembled Chromatin Templates Using a HeLa Cell Ex - http://www.bio.com/protocolstools/protocol.jhtml%3Bjsessionid%3D3APITJJIWFIM1R3FQLMCFEWHUWBNSIV0?id=p9063

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

This protocol describes an in vitro transcription assay that allows for a single round of transcription from in vitro assembled chromatin. Comparing the activity of a receptor or transcriptional coactivator in an assay that measures only a single round of transcription with the results from multiple rounds of transcription can help elucidate the mechanism of transcriptional activation by those factors. - [Read Assay:Single Round of In Vitro Transcription from Assembled Chromatin Templates Using a HeLa Cell Ex]

Assays for B Lymphocyte Function Protocols - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E66340AD6485AA0FEB8F538B2FD389E&objectid=6674A538AC9B642C5CD51556EF7B3D26

Category: Immunology: B Cell Protocols

Describes the antigenic stimulation of in vitro antibody production by B cells and the subsequent measurement of secreted antibodies. The first protocol is a generalized system for inducing in vitro antibody production and can accommodate various types of antigens under study. Secreted antibodies can then be measured with an enzyme-linked immunosorbent assay (ELISA) or other soluble-antibody detection systems. - [Read Assays for B Lymphocyte Function Protocols]

ATPase Assay Kit Protocol - http://www.innovabiosciences.com/technicalresources/protocols/ATP%20assay%20system%20-%20high%20sensitivity7.pdf

Category: Pharmacology and Toxicology Protocols: Drug Discovery

Protocol for ATPase assay kit. - [Read ATPase Assay Kit Protocol]

Automated CellTiter-Blue Cell Viability Assay Protocol - http://www.promega.com/tbs/ep015/ep015.pdf

Category: Cell Biology and Cell Culture: Cell Viability Protocols

Protocol on automated cell titer- blue cell viability assay. - [Read Automated CellTiter-Blue Cell Viability Assay Protocol]

Automated CellTiter-Glo Luminescent Cell Viability Assay Protocol - http://www.promega.com/tbs/ep014/ep014.pdf

Category: Cell Biology and Cell Culture: Cell Viability Protocols

Protocol for the automated cell titer- glo luminescent cell viability assay. - [Read Automated CellTiter-Glo Luminescent Cell Viability Assay Protocol]

ß - GAL Filter Assay Protocol - http://www.fhcrc.org/science/labs/breeden/Methods/b-GAL_filterassay.html

Category: Yeast Protocols: Yeast Genetics Protocols: B-Galactosidase Assay Protocols

Protocol for ß - GAL filter assay. - [Read ß - GAL Filter Assay Protocol]

ß- Galactosidase Assay Protocol - http://www.biochem.northwestern.edu/ibis/morimoto/research/Protocols/VI.%20Transcription/I.%20b-gal%20.pdf

Category: Yeast Protocols: Yeast Genetics Protocols: B-Galactosidase Assay Protocols

Protocol for ß- Galactosidase assay. - [Read ß- Galactosidase Assay Protocol]

B- Galactosidase Assay Protocol - http://www.biochem.northwestern.edu/ibis/morimoto/research/Protocols/VI.%20Transcription/I.%20b-gal%20.pdf

Category: Reporter Gene Assays: B-Galactosidase Assay Protocols

The recommended amount of RSV-ß-Galactosidase plasmid to use for transfection of cells (60 mm or 100 mm dish) is 1-2 µg. The optimal amount of plasmid DNA will be determined by the efficiency of transfection , which is very dependent upon the particular cell line and transfection protocol. - [Read B- Galactosidase Assay Protocol]

B-GAL Filter Assay Protocol - http://www.fhcrc.org/science/labs/breeden/Methods/b-GAL_filterassay.html

Category: Reporter Gene Assays: B-Galactosidase Assay Protocols

Protocol for B-Gal filter assay. Includes Z buffer and X-Gal solution. - [Read B-GAL Filter Assay Protocol]

b-galactosidase Solution Assay Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/B2.html

Category: Immunohistochemistry Protocols

Protocol for b-galactosidase solution assay. - [Read b-galactosidase Solution Assay Protocol]

Band Shift Protocols - http://www.pharma.ethz.ch/institute_groups/biomacromolecules/protocols/bandshift

Category: DNA Protein Interactions Protocols: Electrophoretic Mobility Shift Assays EMSA Protocols

Protocols on band shift. Includes: Phosphorylation of recombinant antibodies; Labeling the antigen with a red fluorophore; Native gel electrophoresis; Band-shift assay for the determination of Kd; Band-shift assay for the determination of koff. - [Read Band Shift Protocols]

Bead Aggregation Assay Protocol - http://www.natureprotocols.com/2006/09/26/bead_aggregation_assay_to_demo.php

Category: Neuroscience Protocols

Bead aggregation assay to demonstrate presynaptic differentiation induced by the NGL family of cell adhesion molecules. - [Read Bead Aggregation Assay Protocol]

Beta b-Galactosidase Color Assay for Cultured Cells - http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/betagal.txt

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Beta b-Galactosidase Color Assay for Cultured Cells. Bradley's Lab, Texas. - [Read Beta b-Galactosidase Color Assay for Cultured Cells]

Beta-gal Assay Protocol - http://www.fhcrc.org/science/labs/gottschling/yeast/Bgal.html

Category: Reporter Gene Assays: B-Galactosidase Assay Protocols

Basic protocol for a Beta-gal assay. - [Read Beta-gal Assay Protocol]

Beta-gal Assay Protocol - http://www.fhcrc.org/science/labs/gottschling/yeast/Bgal.html

Category: Yeast Protocols: Yeast Genetics Protocols: B-Galactosidase Assay Protocols

Protocol for Beta-gal assay. - [Read Beta-gal Assay Protocol]

Beta-galactosidase Reporter Gene Assay (Liquid Form) - http://www.med.unc.edu/~hdohlman/bgal.html

Category: Reporter Gene Assays: B-Galactosidase Assay Protocols

Protocol for Beta-galactosidase reporter gene assay (liquid form). - [Read Beta-galactosidase Reporter Gene Assay (Liquid Form)]

Bioassay for Determining Itraconazole Levels in Blood - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./itrabio.html~main

Category: Fungi Protocols

Serum concentrations of itraconazole should be measured in patients receiving this drug to ensure that therapeutic concentrations are being achieved. This is necessary as drug absorption can be variable, and levels may be lowered by interactions with other drugs. The assay will give an indication of whether suitable blood levels have been achieved. - [Read Bioassay for Determining Itraconazole Levels in Blood]

Biorad Protein Assay Bradford - http://www.musc.edu/BCMB/ceramide/protocols/0019.html

Category: Protein Protocols: Protein Quantitation Concentration Protocols: Bradford Protein Assay Protocols

Biorad Protein Assay Bradford. Contributor: Suprya Jayadev. - [Read Biorad Protein Assay Bradford]

Biuret Protein Assay - http://homepages.gac.edu/~cellab/appds/appd-g.html#exG-3

Category: Protein Protocols: Protein Quantitation Concentration Protocols

Biuret Protein Assay. Includes Materials, methods and procedure. - [Read Biuret Protein Assay]

Bradford Assay Method - http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Biochemistry/Bradford.html

Category: Protein Protocols: Protein Quantitation Concentration Protocols: Bradford Protein Assay Protocols

The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue to protein. There is no interference from cations nor from carbohydrates such as sucrose.
Detergents such as sodium dodecyl sulfate and triton x-100 can interfere with the assay, as well as strongly alkaline solutions.

Includes a general overview of the procedure and preparation of the standards in the protocol. - [Read Bradford Assay Method]

Articles (1-5 of 5)

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Coupling Cysteine Containing Peptides to Carrier Protein for Immunization and Polyclonal Antibody

This protocol is used in the production of polyclonal antibodies that recognize a peptide sequence of interest.

Histone H1 Kinase Activity Assay

Histone H1 Kinase Activity Assay Protocol. This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography.

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