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ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

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Radioactive DNA Fragmentation Assay - http://www.celldeath.de/apometh/dnafragm.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols: DNA Fragmentation Assay

DNA Fragmentation Assay allows determination of the amount of DNA that is degraded upon treatment of cells with treatment of apoptotic agents. Celldeath.de - [Read Radioactive DNA Fragmentation Assay]

Radiolabeling of Probes for Electrophoretic Mobility Shift Assay - http://faculty.virginia.edu/mirmira/resources_files/Protocols/Radiolabeling.htm

Category: DNA Protocols: EMSA DNA-Protein Protocols

Creation of Probe for EMSA using Oligonucleotide annealing, p-32, and T4 Polynucleotide Kinase. - [Read Radiolabeling of Probes for Electrophoretic Mobility Shift Assay]

Raf-1 Kinase Assay - http://www.bio.com/protocolstools/protocol.jhtml?id=p1980

Category: Signalling Signal Transduction Protocols: MAPK Protocols

This protocol uses mitogen-activated protein/ERK kinase (MEK) to activate the extracellular-signal-regulated (Erk) mitogen-activated protein (MAP) kinases upon agonist binding to receptors. Protocol includes information about: Harvest, Immunoprecipitation and Kinase Reaction. Also includes following solutions: Laemmli Sample Buffer (1X), ATP Mix, Kinase Buffer (10X), Lysis Buffer. Helpful protocol hints are also included. - [Read Raf-1 Kinase Assay]

Rat Chromaffin Cells Primary Cultures: Standardization and Quality Assessment for Single-Cell Assay - http://www.natureprotocols.com/2006/09/29/rat_chromaffin_cells_primary_c.php

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

Protocol to be used as reference to help standardization of both the isolation procedure and the quality of the cultures allowing a better comparison of the results obtained from different laboratories. - [Read Rat Chromaffin Cells Primary Cultures: Standardization and Quality Assessment for Single-Cell Assay]

Real-Time PCR (qPCR) Basics - http://web.archive.org/web/20040711051207/http://primerdesign.co.uk/primerdesign/qPCR_basics.html

Category: Real Time PCR

Learn Quantitative PCR Methodology. Assay parameters, Melt Curves and Primer Dimers, Standard Curves, Dynamic Range, Quantification methods, Delta CT, Standard Curves. Dr.Powell. - [Read Real-Time PCR (qPCR) Basics]

Receptor Binding Assay Protocol - http://www1.gelifesciences.com/APTRIX/upp00919.nsf/Content/drugscr_applications~drugscr_applic_technol~drugscr_leadseeker~Radiometric+Application+Sets~mip1a_receptorbinding_assay

Category: Pharmacology and Toxicology Protocols: Receptor Binding Protocols

Protocol for receptor binding assay. - [Read Receptor Binding Assay Protocol]

Red Blood Cell Test System Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/37.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cell Specific Cytotoxicity Assays Protocols

An in vitro red blood cell assay is presented which allows the estimation of the irritation potential of tensides and tenside containing materials such as shampoos, shower gels, cleaning products, etc. The estimation is based on the fact that surfactants interact strongly with cellular membranes and proteins. Both effects are measured photometrically by use of the inherent native dye, oxyhemoglobin. - [Read Red Blood Cell Test System Protocol]

RNA Band Shift Assay. EMSA. - http://www.biochem.uiowa.edu/donelson/Database%20items/rnabndshft.htm

Category: RNA Protocols: RNA-binding Protein EMSA Interactions

Donelson Lab, University of Iowa. - [Read RNA Band Shift Assay. EMSA.]

Rnase Protection Assay Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/R3.html

Category: Molecular Biology Protocols

Protocol for RNAse protection assay. - [Read Rnase Protection Assay Protocol]

SNP Cutter: SNP PCR-RFLP Assay Design - http://bioinfo.bsd.uchicago.edu/SNP_cutter.htm

Category: DNA Protocols: SNP Protocols

SNP Cutter: SNP PCR-RFLP Assay Design. By Ruifang Zhang, Zanhua Zhu, Fengxia Yao, Hongming Zhu, Tu Nguyen, Chunyu Liu - [Read SNP Cutter: SNP PCR-RFLP Assay Design]

SNP Genotyping System - http://www.promega.com/tbs/tm053/tm053.pdf

Category: Genetics and Genomics: Genotyping Protocols

SNP Genotyping System. Includes: Protocol for Manual READIT® Assay in Multiwell Plates; Preparation of L/L Reagent; Preparation of Heating Block; CIAP/Exo Treatment; Preparation of Interrogation Probe Solutions; Template Denaturation; Sample Interrogation; Protocol for Manual Plate Reading Luminometers; Protocol for Injecting Plate Reading Luminometers; Protocol for Automated READIT® Assay with the Biomek® 2000 Instrument. - [Read SNP Genotyping System]

SOLVO In Vitro Transporter Assay Technology - https://beta.solvo.hu/Product%20Families/In%20vitro%20Transporter%20Assays.html

Category: Pharmacology and Toxicology Protocols: Drug Discovery

In Vitro ABC Transporter Assays for Hepato-Biliary DMPK; ABC transporter assays; Uptake transporter assays; Transporter assays for enteral absorption PK; Transporter Assays for Blood-Brain-Barrier PK; In Vitro Transporter Assays for renal excretion. - [Read SOLVO In Vitro Transporter Assay Technology]

Spectrophotometry - http://homepages.gac.edu/~cellab/appds/appd-g.html

Category: General Research Protocols and Methods: Spectrophotometry Protocols

Bradford Protein Assay, Lowry Protein Assay, Biuret Assay. Protein concentration measurement. Dr. Heidcamp, Department of Biology, Gustavus Adolphus College. - [Read Spectrophotometry]

Split Luciferase Complementation Assay for Studying Interaction of Proteins X and Y in Cells - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4596

Category: Reporter Gene Assays: Luciferase Assay Protocols

Protocol describes a split luciferase complementation assay used to study the interaction of proteins in cells. In the split protein strategy, a single reporter protein/enzyme (firefly luciferase [Fluc]) is cleaved into amino-terminal and carboxy-terminal halves; each half is fused to one of two interacting proteins, X & Y. Physical interactions between the two proteins reconstitute the functional reporter protein, leading to enzymatic activities that can be measured by in vitro or in vivo assay - [Read Split Luciferase Complementation Assay for Studying Interaction of Proteins X and Y in Cells]

Split Luciferase Complementation Assay for Studying Interaction of Proteins X and Y in Living Mice - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4595

Category: Reporter Gene Assays: Luciferase Assay Protocols

Protocol describes a split luciferase complementation assay that can be used to repetitively and noninvasively study the interaction of proteins in small living animals. After the expression of the appropriate vectors has been checked in cell culture in vivo, studies can be performed either by implanting transiently transfected cells for short-term analysis (maximum of 7 days), or with tumor models grown from tumor cells stably expressing the complete reporter system. - [Read Split Luciferase Complementation Assay for Studying Interaction of Proteins X and Y in Living Mice]

Sumoylation and Desumoylation Assays for a Chromatin-Remodelling Complex In Vivo and In Vitro - http://www.natureprotocols.com/2006/11/09/sumoylation_and_desumoylation.php

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Small ubiquitin-like modifier (SUMO) is a small molecule, but has a variety of regulatory functions in cells. SUMO modification is involved in transcriptional regulation, subcellular localization, and protein-protein interactions. SUMO conjugation requires sequential E1-dependent activation, E2-dependent conjugation, and E3-dependent ligation steps. Protocol includes: In vivo and in vitro SUMOylation assay and deSUMOylation assay. - [Read Sumoylation and Desumoylation Assays for a Chromatin-Remodelling Complex In Vivo and In Vitro]

TaqMan Assay on CYP2C9 Protocol - http://www.pharmgkb.org/do/serve?objId=PA149569824&objCls=TaqmanAssay

Category: Pharmacology and Toxicology Protocols: Drug Metabolism Protocols

Protocol for TaqMan assay on CYP2C9. - [Read TaqMan Assay on CYP2C9 Protocol]

Target Specific Whole Cell Assay for Antibacterial Drug Discovery Protocol - http://www.natureprotocols.com/2006/06/23/a_targetspecific_whole_cell_as.php

Category: Pharmacology and Toxicology Protocols: Drug Discovery

Protocol describes a target selective S. aureus whole cell assay that combines agar-diffusion and protein over expression techniques. This agar based two-plate differential sensitivity assay was used to help confirm the newly discovered antibiotic platensimycin inhibited bacterial growth by specifically targeting the essential FASII enzyme FabF6. - [Read Target Specific Whole Cell Assay for Antibacterial Drug Discovery Protocol]

The Under-Agarose Migration Assay - http://bioprotocol.bio.com/protocolstools/protocol.jhtml?id=p2157

Category: Signalling Signal Transduction Protocols: Chemokine Protocols

Under-Agarose assay is a useful method for observing the response of a cell population to one or more chemoattractant sources. BioProtocol - [Read The Under-Agarose Migration Assay]

Transient Transfection and Luciferase assay - http://www.natureprotocols.com/2006/10/27/transient_transfection_and_luc.php

Category: Transfection Protocols: Transient Transfection Protocols

Transient transfection into mammalian cells is a convenient way to over express and obtain protein expression. Protocol includes: Culture conditions; Transfection of experimental cells; Preparation of Mammalian Cell Lysate for Luciferase assay. - [Read Transient Transfection and Luciferase assay]

Transient Transfection and Luciferase Assay Protocol - http://www.natureprotocols.com/2006/10/27/transient_transfection_and_luc.php

Category: Reporter Gene Assays: Luciferase Assay Protocols

Ubiquitin Assay Protocol for Yeast - http://tanseylab.cshl.edu/pdf/His_Ub_Yeast.pdf

Category: Protein Protocols: Protein Ubiquitination Protocols

Ubiquitin Assay Protocol for Yeast. Tansey Lab. - [Read Ubiquitin Assay Protocol for Yeast]

Ultimate His-UB Assay for Mammalian Cells - http://tanseylab.cshl.edu/pdf/His_Ub_mammals.pdf

Category: Protein Protocols: Protein Ubiquitination Protocols

Ultimate His-UB Assay for Mammalian Cells. Tansey Lab Protocols. William P. Tansey. PREPARATION OF NI-NTA-AGAROSE, HARVESTING THE TRANSFECTION., ELUTION OF HIS-TAGGED PROTEINS. - [Read Ultimate His-UB Assay for Mammalian Cells]

Use of the Bradford Protein Assay in a Microtiter Plate Format - http://www.animal.ufl.edu/hansen/protocols/minibradford.htm

Category: Protein Protocols: Protein Quantitation Concentration Protocols: Bradford Protein Assay Protocols

Use of the Bradford Protein Assay in a Microtiter Plate Format Protocol. Includes a short introduction to the Bradford assy, materials and procedure. - [Read Use of the Bradford Protein Assay in a Microtiter Plate Format]

V79 Cytotoxicity Test for Membrane Damage - http://embryo.ib.amwaw.edu.pl/invittox/prot/39.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols

The cytotoxic effect of test chemicals in V79 cell culture can be determined by assessing damage to the plasma membrane as determined by a nucleic acid leakage assay. - [Read V79 Cytotoxicity Test for Membrane Damage]

Articles (1-5 of 5)

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Coupling Cysteine Containing Peptides to Carrier Protein for Immunization and Polyclonal Antibody

This protocol is used in the production of polyclonal antibodies that recognize a peptide sequence of interest.

Histone H1 Kinase Activity Assay

Histone H1 Kinase Activity Assay Protocol. This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography.

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