User Panel

My Panel

Bookmark Science Articles

aspergillus Protocols

Search results for: aspergillus

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

A Rapid Method for Generating Gene Deletions in Aspergillus fumigatus Protocol - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./genedel.html~main

Category: Fungi Protocols: Aspergillus Protocols

The technique makes use of an Escherichia coli strain expressing the redΑßΓ operon under the control of an inducible promoter. This enables the strain to carry out homologous recombination with only 50-60 bp of homologous sequence. The procedure does not require any DNA ligation and is very rapid. It allows a single gene or region on a cosmid to be replaced by a bi-functional selectable marker (having both an E. coli and an A. fumigatus marker). - [Read A Rapid Method for Generating Gene Deletions in Aspergillus fumigatus Protocol]

A Rapid Method for Isolation of Total Nucleic Acids from Aspergillus nidulans Protocol - http://www.fgsc.net/fgn/chow.html

Category: Fungi Protocols: Aspergillus Protocols

Protocol for the rapid method for isolation of total nucleic acids from Aspergillus nidulans. - [Read A Rapid Method for Isolation of Total Nucleic Acids from Aspergillus nidulans Protocol]

Aspergillus nidulans Mutants - http://www.fgsc.net/alist.html

Category: Fungi Protocols: Aspergillus Protocols

Compilation of A. nidulans mutants listing names, affected enzymes (or gene functions), linkage assignments, properties of the mutants, and names of corresponding loci in N. crassa. - [Read Aspergillus nidulans Mutants]

Bioassay for Determining Itraconazole Levels in Blood - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./itrabio.html~main

Category: Fungi Protocols

Serum concentrations of itraconazole should be measured in patients receiving this drug to ensure that therapeutic concentrations are being achieved. This is necessary as drug absorption can be variable, and levels may be lowered by interactions with other drugs. The assay will give an indication of whether suitable blood levels have been achieved. - [Read Bioassay for Determining Itraconazole Levels in Blood]

Detection of Fungi by Fluorescence Microscopy Using Fluorescent Brighteners - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./flbr.html~main

Category: Microscopy Protocols: Fluorescence Microscopy Protocols

Certain fluorescent dyes such as Blankophor have a high affinity for the b -glycosidically linked polysaccharides such as glucan and chitin, which are main the constituents of the fungal cell wall. Therefore, these fluorescent dyes can be used for screening clinical samples for the presence of fungal elements. This procedure can be performed using the following specimens: Nail, Skin, Bronchial alveolar lavage fluid, Sputum and Biopsies. - [Read Detection of Fungi by Fluorescence Microscopy Using Fluorescent Brighteners]

Detection of Fungi by Fluorescence Microscopy Using Fluorescent Brighteners Protocol - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./flbr.html~main

Category: Fungi Protocols

Protocol for the detection of fungi by fluorescence microscopy using fluorescent brighteners. - [Read Detection of Fungi by Fluorescence Microscopy Using Fluorescent Brighteners Protocol]

Fundamentals of Growth, Storage, Genetics and Microscopy of Aspergillus nidulans Protocols - http://www.fgsc.net/fgn48/Kaminskyj.htm

Category: Fungi Protocols: Aspergillus Protocols

Compendium of protocols for using Aspergillus nidulans in genetic, molecular, and cell biological investigations, originally written for members of my research group. It also summarizes our common growth media and nutritional supplements, many of which originally appeared elsewhere but now are difficult to locate. Includes: Growth and storage of Aspergillus nidulans conidia; Nutritional supplements for our common auxotrophies; Double mutants; Mitotic mapping - assigning genes to chromosomes; etc - [Read Fundamentals of Growth, Storage, Genetics and Microscopy of Aspergillus nidulans Protocols]

Media for Culture of Aspergillus nidulans Protocol - http://www.fgsc.net/methods/anidmed.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Yeast Cell Culture Growth Media Protocols

Protocol for media for culture of Aspergillus nidulans. Includes minimal and complete media. - [Read Media for Culture of Aspergillus nidulans Protocol]

Media for Culture of Aspergillus nidulans Protocol - http://www.fgsc.net/methods/anidmed.html

Category: Fungi Protocols: Aspergillus Protocols

Protocol for media for culture of Aspergillus nidulans. Includes: Minimal medium; Complete media. - [Read Media for Culture of Aspergillus nidulans Protocol]

Method for Assaying Gliotoxin Production in Aspergillus fumigatus Protocol - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./gliotoxin1.html~main

Category: Fungi Protocols: Aspergillus Protocols

Gliotoxin is a metabolite of Aspergillus fumigatus that exhibits immunosuppressive activity against certain cells of the immune system. Secretion of gliotoxin during infection has been suggested as being a factor in the pathogenesis of aspergillosis. Gliotoxin secretion can be assayed in a number of ways by thin layer chromatography (TLC) high performance liquid chromatography (HPLC) or bioassay using the effect of gliotoxin on human cells1. - [Read Method for Assaying Gliotoxin Production in Aspergillus fumigatus Protocol]

Phagocytosis Assay Protocol - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./protocol5.html~main

Category: Fungi Protocols

Protocol for phagocytosis assay. Protocol describes how to assess the efficiency of phagocytic cells to engulf conidia of a given fungus. - [Read Phagocytosis Assay Protocol]

Preparation of Solid Tissue for Aspergillus Galactomannan Antigen Detection by Platelia Protocol - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./galactoman.html~main

Category: Fungi Protocols

Protocol for the preparation of solid tissue for Aspergillus galactomannan antigen detection by Platelia (Biorad). Technique was designed for use on human serum. However, it may also be possible to perform this method on solid tissues and organic solutions. Viscous solution and tissue specimens need to be pre-treated to achieve the extraction of the Aspergillus antigen and to get a homogeneous sample in solution. - [Read Preparation of Solid Tissue for Aspergillus Galactomannan Antigen Detection by Platelia Protocol]

Protocol: Bioassay for Determining Voriconazole Levels in Blood - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./BIOASSAY.html~main

Category: Fungi Protocols

Serum concentrations of voriconazole should be measured in patients receiving this drug to ensure that therapeutic levels are being achieved. The assay will give an indication of whether suitable blood levels have been achieved. - [Read Protocol: Bioassay for Determining Voriconazole Levels in Blood]

Protoplast Preparation for A nidulans Protocol - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./protoplastRL.html~main

Category: Fungi Protocols: Aspergillus Protocols

Protocol for protoplast preparation for A nidulans. - [Read Protoplast Preparation for A nidulans Protocol]

Purification of Monocytes Protocol - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./protocols.html~main

Category: Immunology: Mononuclear Cell Isolation Protocols

Protocol for the purification of monocytes. - [Read Purification of Monocytes Protocol]

Purification of Neutrophils Protocol - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./protocol1.html~main

Category: Immunology: Mononuclear Cell Isolation Protocols

Protocol for purification of neutrophils. - [Read Purification of Neutrophils Protocol]

Random Amplification of Polymorphic DNA (RAPD) Typing and Fingerprinting Protocol - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./rapds.html~main

Category: Fungi Protocols: Aspergillus Protocols

RAPD is a procedure for typing and fingerprinting isolates of a species. It can be used for epidemiological studies, such as investigations into hospital outbreaks and as a laboratory aid to keep track of cultures and to verify that mutants generated in the laboratory are genetically identical to the parental strain. In our hands, the use of one primer, R108, is sufficiently discriminatory to distinguish between the isolates of different strains. - [Read Random Amplification of Polymorphic DNA (RAPD) Typing and Fingerprinting Protocol]

Sampling of Aspergillus spp Spores in Air Protocol - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./airprotocol.html~main

Category: Fungi Protocols: Aspergillus Protocols

Protocol allows the isolation and enumerate Aspergillus spp spores in air. Includes: Sampling Procedure; Sampling Location; Selection of Sampling Time; Sampling Steps; Laboratory Procedure; Enumerating the Colony Forming Units. - [Read Sampling of Aspergillus spp Spores in Air Protocol]

Transformation of Aspergillus niger Protocol - http://clone.concordia.ca/aspergillus/protocols/nigtransf.html

Category: Fungi Protocols: Aspergillus Protocols

Protocol for the transformation of Aspergillus niger. This procedure is done by first digesting the outer cell wall, forming protoplasts, and then by making holes in the membrane through which the dna can enter using calcium chloride and polyethylene glycol. Includes: Protocol for making A.niger protoplasts; Transformation; Plating. - [Read Transformation of Aspergillus niger Protocol]