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How to Avoid Pitfalls and Keep Out of Trouble in the Neurospora Lab - http://www.fgsc.net/neurosporaprotocols/How%20to%20avoid%20pitfalls%20and%20keep%20out%20of%20trouble.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to avoid pitfalls and keep out of trouble in the Neurospora lab. Includes: Culture, incubation, storage, and disposal; Stock preservation; Spore and colony counts; Making crosses and recovering progeny; Use of heterokaryons for allelism tests; Use of unordered asci to estimate the relation of translocation breakpoints to centromeres. - [Read How to Avoid Pitfalls and Keep Out of Trouble in the Neurospora Lab]

How to Obtain Asci as Unordered Groups of Ascospores Ejected From the Perithecium - http://www.fgsc.net/neurosporaprotocols/How%20to%20obtain%20asci%20as%20unordered%20groups%20of%20ascospores%20ejected%20from%20the%20perithecium.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to obtain asci as unordered groups of ascospores ejected from the perithecium. - [Read How to Obtain Asci as Unordered Groups of Ascospores Ejected From the Perithecium]

How to Obtain Unbroken Linear Asci that are Extruded Individually From the Perithecium - http://www.fgsc.net/neurosporaprotocols/How%20to%20obtain%20unbroken%20linear%20asci%20that%20are%20extruded%20individually%20from%20the%20perithecium.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to obtain unbroken linear asci that are extruded individually from the perithecium. - [Read How to Obtain Unbroken Linear Asci that are Extruded Individually From the Perithecium]

How to Use Asci for Obtaining Double Mutants - http://www.fgsc.net/neurosporaprotocols/How%20to%20use%20asci%20for%20obtaining%20double%20mutants%20of%20genes%20that%20show%20epistasis%20of%20are%20phenotypically%20similar.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to use asci for obtaining double mutants of genes that show epistasis or are phenotypically similar. - [Read How to Use Asci for Obtaining Double Mutants]

How to Use Spore Killer for Distinguishing 1st- and 2nd-Division Segregation with Unordered Asci - http://www.fgsc.net/neurosporaprotocols/How%20to%20use%20Spore%20killer%20for%20distinguishing%201st-%20and%202nd-division%20segregation%20with%20unordered%20asci.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to use Spore killer for distinguishing 1st- and 2nd-division segregation with unordered asci. - [Read How to Use Spore Killer for Distinguishing 1st- and 2nd-Division Segregation with Unordered Asci]

Methylation-Specific Digital Karyotyping Protocol - http://www.natureprotocols.com/2006/11/09/methylationspecific_digital_ka.php

Category: Cytogenetics Protocols

Protocol describes a recently developed method — methylation-specific digital karyotyping (MSDK) — that enables comprehensive and unbiased genome-wide DNA methylation analysis. Using a combination of a methylation-sensitive mapping enzyme (for example, AscI) and a fragmenting enzyme (for example, NlaIII), short sequence tags can be obtained and uniquely mapped to genome location. - [Read Methylation-Specific Digital Karyotyping Protocol]