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Antibody Array Production and Analysis - http://www.stanford.edu/group/robinsonlab/protocols/protocols_tools.htm

Antibody Array Production and Data Analysis. Probing Antigen Arrays and washing/ Blocking. Great Protocols and information. Brian Kidd. Stanford. - [Read Antibody Array Production and Analysis]

C. elegans Integrating Extrachromasomal Arrays Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=4

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol and information related to C. elegans integrating extrachromasomal arrays. - [Read C. elegans Integrating Extrachromasomal Arrays Protocol]

DIG Labeling of cDNA Probes Protocol - http://home.cc.umanitoba.ca/~frist/Protocols/arrays/DIG_probes.html

Category: Cytogenetics Protocols: DIG Biotin Labeled Probe Protocols

Protocol for DIG labeling of cDNA probes. Includes: Incorporation DIG during PCR; Estimating the yield of DIG labeled probes; DNA Dot Blotting; Prehybridization and Hybridization. - [Read DIG Labeling of cDNA Probes Protocol]

DIG Labeling of cDNA Probes Protocol - http://home.cc.umanitoba.ca/~frist/Protocols/arrays/DIG_probes.html

Category: Nucleic Acid Modification Protocols: DIG Biotin Labeled Probes Protocols

Genome-Wide Analysis Protocol - http://www.natureprotocols.com/2006/06/29/genomewide_analysis_of_data_ge.php

Category: Microarray Protocols: Microarray Analysis Protocols

Genome-wide analysis of data generated on the Affymetrix 10K Xba 142 arrays for identification of regions with high probability to contain genes responsible for Micronodular (non-pigmented) Adrenocortical Hyperplasia. - [Read Genome-Wide Analysis Protocol]

Human DNA Microarray Hybridization - http://cmgm.stanford.edu/pbrown/protocols/5_hyb_human.html

Category: DNA Microarray Protocols

Preparation of Fluorescent DNA Probe from HUMAN mRNA or Total RNA using Direct Incorporation Washing and Scanning Arrays. Brown Lab. - [Read Human DNA Microarray Hybridization]

Hybridization of Arrays - http://cmgm.stanford.edu/pbrown/protocols/5_hyb_yeast.html

Category: DNA Microarray Protocols

Hybridization of Arrays. . General/Yeast Microarrays. Brown Lab - [Read Hybridization of Arrays]

Integrating Extrachromasomal Arrays into the C. elegans Chromosomes Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=4

Category: Cloning Protocols: Mutagenesis Protocols

Protocol for integrating extrachromasomal arrays into the C. elegans chromosomes. - [Read Integrating Extrachromasomal Arrays into the C. elegans Chromosomes Protocol]

Microarray Protocol for Affymetrix In Situ Synthesized Oligo Arrays - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000174.pdf?pid=PP00000174

Category: Microarray Protocols: Microarray Hybridization Protocols

Protocol details the preparation of biotin-labeled target samples and hybridization of these samples to an Affymetrix in situ synthesized oligonucleotide GeneChip array. The procedure requires a minimum of 5 µg of purified total RNA as starting material. - [Read Microarray Protocol for Affymetrix In Situ Synthesized Oligo Arrays]

Microarray Protocol for Agilent Inkjet-Deposited Presynthesized Oligo Arrays - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000179.pdf?pid=PP00000179

Category: Microarray Protocols: Microarray Hybridization Protocols

Protocol details the preparation of fluorescently labeled target samples and hybridization of these samples to a microarray of Agilent inkjet-deposited presynthesized oligonucleotides. The procedure requires a minimum of 3 µg of purified total RNA as starting material. Includes: cDNA Synthesis; Fluorescent cRNA Synthesis; cRNA Precipitation and Cleanup; cRNA Quantification; Hybridization; Washing. - [Read Microarray Protocol for Agilent Inkjet-Deposited Presynthesized Oligo Arrays]

PCR protocol for cDNA Arrays on Membranes. PDF file. - http://www.daf.jhmi.edu/microarray/protocols/protocol6.pdf

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

PCR protocol for cDNA Arrays on Membranes. PDF file - [Read PCR protocol for cDNA Arrays on Membranes. PDF file.]

PCR protocol for cDNA Arrays on Membranes. PDF file. - http://www.daf.jhmi.edu/microarray/protocols/protocol6.pdf

Category: PCR Protocols

PCR protocol for cDNA Arrays on Membranes. PDF file - [Read PCR protocol for cDNA Arrays on Membranes. PDF file.]

Post-Processing of Arrays - http://cmgm.stanford.edu/pbrown/protocols/3_post_process.html

Category: DNA Microarray Protocols

Post-Processing of Arrays. Brown Lab. - [Read Post-Processing of Arrays]

Preparation of Fluorescent DNA Probe from HUMAN mRNA or Total RNA using Direct Incorporation - http://cmgm.stanford.edu/pbrown/protocols/5_hyb_human.html

Category: Nucleic Acid Detection Protocols

Protocol for preparation of fluorescent DNA probe from HUMAN mRNA or total RNA using direct incorporation. Includes: Preparing fluoresenctly labeled cDNA (probe); Washing and Scanning Arrays. - [Read Preparation of Fluorescent DNA Probe from HUMAN mRNA or Total RNA using Direct Incorporation]

Preparation of Fluorescent DNA Probe from HUMAN mRNA/ Total RNA using Direct Incorporation Protocol - http://cmgm.stanford.edu/pbrown/protocols/5_hyb_human.html

Category: Microarray Protocols: Microarray Hybridization Protocols

Protocol for the preparation of fluorescent DNA probe from HUMAN mRNA or total RNA using direct incorporation. Includes: Preparing fluoresenctly labeled cDNA (probe); Washing and Scanning Arrays. - [Read Preparation of Fluorescent DNA Probe from HUMAN mRNA/ Total RNA using Direct Incorporation Protocol]

Articles (1-3 of 3)

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.