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Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF Protocol - http://crutcherlab.med.uc.edu/ELI.htm

Protocol for ELISA assay for NGF. Includes: ABSORPTION OF THE POLYCLONAL AND PREIMMUNE SERUM; BLOCKING; SAMPLE PREPARATION; PREPARATION OF NGF STANDARDS; PROTEIN RECOVERY; DESIGNING THE PLATE; APPLYING THE STANDARDS AND SAMPLES; APPLYING THE MONOCLONAL; APPLYING SECONDARY ANTIBODY; APPLYING STREPTAVIDIN; CHROMAGEN DEVELOPMENT; READING THE PLATE. - [Read Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF Protocol]

Gel Filtration Column Chromatography - http://www4.nau.edu/biology/bio349l/Documents/bio349l/Protocols_for_week_3.pdf

Category: Chromatography Protocols: Gel Filtration Chromatography Protocols

Gel Filtration Column Chromatography Protocol. Includes information on: Calibrating the column, Applying standards to the column, Running SDS-PAGE Gels (Tris-Tricine â€œSchÃ¤ggerâ€ Gels) and includes questions. North Arizona University. - [Read Gel Filtration Column Chromatography]

Live Imaging of Caenorhabditis elegans: Preparation of Samples - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4601

Category: Microscopy Protocols: In Vivo Imaging Protocols

Caenorhabditis elegans, a small (adults are ~1 mm long), free-living soil nematode that feeds on bacteria, is an ideal organism for applying various live microscopy techniques. This protocol describes useful techniques for preparing C. elegans for live microscopic analysis. Details of sample preparation depend on the developmental stage of the worm to be studied. - [Read Live Imaging of Caenorhabditis elegans: Preparation of Samples]