apparatus Protocols

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Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber) - http://bioprotocol.bio.com/protocolstools/protocol.jhtml?id=p2158

Category: Signalling Signal Transduction Protocols: Chemokine Protocols

Simple apparatus to study chemotaxis of leukocytes or other migratory cells. Protocol for Chemotaxis. BioProtocol - [Read Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber)]

Electroporation of Eukaryotic Cells - http://omrf.ouhsc.edu/~frank/EPOREUK.html

Category: Cell Biology and Cell Culture: Cell Electroporation Protocols

The protocol uses a Bio-Rad Gene Pulser II apparatus to electroporate DNA into human cell lines. Dr. Frank, Arthritis and Immunology Program, Oklahoma City Medical Research Foundation - [Read Electroporation of Eukaryotic Cells]

SDS POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE) - http://www.mcb.uct.ac.za//sdspage.htm

Category: Protein Protocols: Protein Electrophoresis: SDS-PAGE Electrophoresis Protocols

SDS POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE). Ed Rybicki and Maud Purves. Dept Microbiology, University of Cape Town. PROTOCOL includes: Assembling Gel Apparatus, Resolving Gels, Stacking Gels, Electrophoresis buffer, Sample Preparation for Sds-page and Staining of Gels. - [Read SDS POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE)]

Total Internal Reflection Microscopy - http://www.andrew.cmu.edu/user/dcprieve/TIRM.htm

Category: Microscopy Protocols: Optical Microscopy Protocols

TIRM is a optical technique for monitoring the instantaneous separation distance between a microscopic sphere & a flat plate. Changes in distance as small as 1 nm can be detected. Includes information on: Scattering Intensity I is Related to Elevation h ; apparatus. - [Read Total Internal Reflection Microscopy]

Western Blot Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/W1.html

Category: Western Blot Protocols: General Western Blot Methods Protocols

This protocol was developed for the BIORAD protein gel and transfer apparatus. The buffers can be used with any electrophoresis/transfer system. - [Read Western Blot Protocol]