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Tips and Hints for the Storage of Antibodies - http://www.sysy.com/faqs/storage.html

Category: Antibody Protocols: Antibody Production Protocols

Tips and hints for the storage of antibodies. Includes: Storage of lyophilized antibodies; Monoclonal antibodies; Rabbit antisera; Polyclonal affinity purified antibodies. - [Read Tips and Hints for the Storage of Antibodies]

Using FLAG Epitope-Tagged Proteins for Coimmunoprecipitation of Interacting Proteins Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/28/pdb.prot4557

Category: Immunology: Immunoprecipitation Protocols: Protein Immunoprecipitation Protocols

Protocol describes the use of FLAG-epitope-tagged proteins for both small-scale analysis and large-scale coimmunoprecipitation of interacting proteins. When examining protein interactions, it is sometimes possible to immunoprecipitate an endogenous protein X directly, without using an epitope tag, if antibodies are available. The advantage of examining interactions of endogenously expressed proteins is that these are likely to be physiological and less likely to be an artifact of overexpression. - [Read Using FLAG Epitope-Tagged Proteins for Coimmunoprecipitation of Interacting Proteins Protocol]

Western Blot Analysis—Phosphoprotein-Specific Antibody Mixture Protocol - http://www.afcs.org/reports/v1/BC0001/Protocols/WBPhosphoSpecific.pdf

Category: Immunology: Immunoassays

3-step process of Western immunoblotting for detection of particular sites of phosphorylation on specific proteins recognized by modification-sensitive antibodies. - [Read Western Blot Analysis—Phosphoprotein-Specific Antibody Mixture Protocol]

Western Blotting with Biotinylated Antibodies - http://www.bdbiosciences.com/pharmingen/protocols/Biotinylated_Antibodies.shtml

Category: Protein Protocols: Western Blot Protocol

Detailed protocol for Western Blot using Biotinylated Antibodies. (BD PharMingen). - [Read Western Blotting with Biotinylated Antibodies]

Western Blotting with Monoclonal Antibodies - http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Category: Protein Protocols: Western Blot Protocol

Detailed General Protocol for Western Blotting with Monoclonal Antibodies. PharMingen - [Read Western Blotting with Monoclonal Antibodies]

Whole Mount Fluorescence in Situ Hybridization (FISH) of Repetitive DNA Sequences on Interphase - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_220-228.pdf

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

Protocol for whole mount fluorescence in situ hybridization (FISH) of repetitive DNA sequences on interphase nuclei of the small cruciferous plant Arabidopsis thaliana. Includes: Seed sterilization and germination; Tissue fixation; Labeling of the probe DNA; Pretreatment; In situ hybridization; Pre-absorption of antibodies; Posthybridization washes; Immunocytochemical detection; Direct detection; Indirect detection; Staining and mounting; Fluorescence microscopy. - [Read Whole Mount Fluorescence in Situ Hybridization (FISH) of Repetitive DNA Sequences on Interphase]

Articles (1-4 of 4)

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.