anion Protocols

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Purification of DNA Recovered Anion-exchange Chromatography Protocol - http://cshprotocols.cshlp.org/cgi/content/extract/2006/2/pdb.prot4024

Category: Chromatography Protocols: Ion Exchange Chromatography Protocols

Protocol for the purification of DNA recovered from agarose and polyacrylamide gels by anion-exchange chromatography. Fragments of DNA recovered from agarose gels are sometimes poor templates or substrates in subsequent enzymatic reactions. This problem can be solved by binding the DNA to a positively charged matrix, such as DEAE-Sephadex or DEAE-Sephacel, in buffers of low ionic strength. After washing the matrix, the DNA is eluted by raising the strength of the buffer. - [Read Purification of DNA Recovered Anion-exchange Chromatography Protocol]

Selection of an Ion Exchanger Protocol - http://cshprotocols.cshlp.org/cgi/content/extract/2006/1/pdb.prot4192

Category: Chromatography Protocols: Ion Exchange Chromatography Protocols

Protocol for selection of an ion exchanger: Determining the pI of a protein using isoelectric focusing. The choice of whether to use an anion or a cation exchanger should be based on knowledge of the stability of the protein, and the binding properties of the target protein and other molecules present. - [Read Selection of an Ion Exchanger Protocol]

Selection of an Ion Exchanger Protocol II - http://cshprotocols.cshlp.org/cgi/content/extract/2006/1/pdb.prot4191

Category: Chromatography Protocols: Ion Exchange Chromatography Protocols

Protocol for selection of an ion exchanger: Determining the pI of a protein using the titration curve method. The choice of whether to use an anion or a cation exchanger should be based on knowledge of the stability of the protein, and the binding properties of the target protein and other molecules present in the sample. - [Read Selection of an Ion Exchanger Protocol II]

Selection of an Ion Exchanger Protocol III - http://cshprotocols.cshlp.org/cgi/content/extract/2006/1/pdb.prot4193

Category: Chromatography Protocols: Ion Exchange Chromatography Protocols

Protocol for selection of an ion exchanger: Determining the pI of a protein using the test tube method. The choice of whether to use an anion or a cation exchanger should be based on knowledge of the stability of the protein, and the binding properties of the target protein and other molecules present in the sample. - [Read Selection of an Ion Exchanger Protocol III]

Selection of an Ion Exchanger Protocol IV - http://cshprotocols.cshlp.org/cgi/content/extract/2006/1/pdb.prot4194

Category: Chromatography Protocols: Ion Exchange Chromatography Protocols

Protocol for selection of an ion exchanger: Determining the pI of a protein using the Trial-and-Error method. The choice of whether to use an anion or a cation exchanger should be based on knowledge of the stability of the protein, and the binding properties of the target protein and other molecules present in the sample. - [Read Selection of an Ion Exchanger Protocol IV]